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Diss Factsheets
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EC number: 231-901-9 | CAS number: 7778-39-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- From February 29 to April 12, 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- GLP study conducted similarly to OECD Guideline 406 with deviations: less no. of animals used in treatment group; concentrations applied in induction phase caused mortalities and strong dermal reactions. Interpretaion of the results may have been false.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 406 (Skin Sensitisation)
- Deviations:
- yes
- Remarks:
- less no. of animals used in treatment group; concentrations applied in induction phase caused mortalities and strong dermal reactions
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 81-6 (Skin Sensitisation)
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes
- Type of study:
- Buehler test
Test material
- Reference substance name:
- Arsenic acid
- EC Number:
- 231-901-9
- EC Name:
- Arsenic acid
- Cas Number:
- 7778-39-4
- Molecular formula:
- H3AsO4
- IUPAC Name:
- arsoric acid
- Details on test material:
- - Name of test material (as cited in study report): Arsenic acid
- Physical state: Light green liquid
- Analytical purity: 75%
- Lot/batch No.: 8619
- Storage condition of test material: Room temperature
Constituent 1
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- Dunkin-Hartley
- Sex:
- male
Study design: in vivo (non-LLNA)
Inductionopen allclose all
- Route:
- epicutaneous, occlusive
- Vehicle:
- water
- Concentration / amount:
- Range finding test: Topical induction exposure
- Experiment 1: 25, 50, 75 or 100% w/v
- Experiment 2: 0.1, 1, 10 and 25% w/v
Main test:
- Topical induction exposure: 25% w/v (first two doses) and 10% w/v (last dose)
- Topical challenge exposure: 1% w/v
Challengeopen allclose all
- Route:
- epicutaneous, occlusive
- Vehicle:
- water
- Concentration / amount:
- Range finding test: Topical induction exposure
- Experiment 1: 25, 50, 75 or 100% w/v
- Experiment 2: 0.1, 1, 10 and 25% w/v
Main test:
- Topical induction exposure: 25% w/v (first two doses) and 10% w/v (last dose)
- Topical challenge exposure: 1% w/v
- No. of animals per dose:
- - Range finding test: 4 males
- Main test: 10 males (test and untreated control group), 4 males (positive control group)
Results and discussion
In vivo (non-LLNA)
Resultsopen allclose all
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 25 or 10% w/v in deionised water
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Clinical observations:
- 2/10 animals died within 10 days; surviving animals appeared normal at study termination
- Remarks on result:
- other: see Remark
- Remarks:
- Reading: 1st reading. . Hours after challenge: 24.0. Group: test group. Dose level: 25 or 10% w/v in deionised water . No with. + reactions: 0.0. Total no. in groups: 10.0. Clinical observations: 2/10 animals died within 10 days; surviving animals appeared normal at study termination.
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 25 or 10% w/v in deionised water
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Clinical observations:
- 2/10 animals died within 10 days; surviving animals appeared normal at study termination
- Remarks on result:
- other: see Remark
- Remarks:
- Reading: 2nd reading. . Hours after challenge: 48.0. Group: test group. Dose level: 25 or 10% w/v in deionised water. No with. + reactions: 0.0. Total no. in groups: 10.0. Clinical observations: 2/10 animals died within 10 days; surviving animals appeared normal at study termination.
Any other information on results incl. tables
- 2/10 animals died within 10 days; surviving animals appeared normal at study termination
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information
- Conclusions:
- Under these test conditions, arsenic acid is not classified according to the Annex VI of the Directive 67/548/EEC and the CLP Regulation (EC) No. 1272/2008.
- Executive summary:
In a Buehler closed patch study conducted similarly to OECD Guideline 406 and in compliance with GLP, a group of 10 male Dunkin-Hartley guinea pigs were topically induced with arsenic acid (0.4 mL) at a concentration of 25% w/v (first two doses) or 10% w/v in deionised water (last dose) via occlusive patch for 6 hours, followed by a rest period of 2 weeks and then with a challenge patch of 1% w/v. Ten naive animals were applied with a challenge patch in the same manner. Four animals in the positive control group were applied with three induction patches of 0.3% w/v 2,4-dinitrochlorobenzene (DNCB) in 80% ethanol/deionised water followed by a challenge patch of 0.1% w/v DNCB in acetone. The test concentrations for the main study were determined from the range-finding studies conducted at concentrations of 25, 50, 75 and 100% w/v (study 1) and 0.1, 1, 10 and 25% w/v in deionised water (study 2).
Topical induction indicated evidence of mild to strong dermal reactions. No skin reactions were noted at the challenge sites of the test or untreated control group animals at the 24 or 48-hour observations. Arsenic acid produced a 0% (0/20) sensitisation rate and was considered to be a nonsensitiser to guinea pig skin. Positive control exhibited evidence of sensitisation, thus confirming the susceptibility of this group of animals to dermal sensitisation.
Under these test conditions, arsenic acid is not classified according to the Annex VI of the Directive 67/548/EEC and the CLP Regulation (EC) No. 1272/2008. However this study is not considered as acceptable as it does not satisfy the guideline requirement for a skin sensitisation study.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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