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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 231-901-9 | CAS number: 7778-39-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The test is conducted according to a protocol different of the OECD guidelines. However the result is considered as reliable enough to validate the endpoint.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 976
- Report date:
- 1976
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- Deviations:
- yes
- GLP compliance:
- not specified
Test material
- Reference substance name:
- di-Natriumhydrogenarsenat
- IUPAC Name:
- di-Natriumhydrogenarsenat
- Details on test material:
- - Name of test material (as cited in study report):di-natriumhydrogenarsenate
- Molecular formula (if other than submission substance): Na2HAsO4 7h2O
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
no additionnal data
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- not relevant
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the test solution is first neutralized, using as few soda as possible. No data on test substance dissolution.
- Eluate: bidistilled water
- Differential loading:The dilution of the stock solution is made with a factor of 2 exp 0 to 2exp14 the first container contains 160 mL of the test substance solution and the further containers of the serie contains 80 mL of the previous solution and 80 mL of distilled water.
- Controls: test medium
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):none
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)):not relevant
- Evidence of undissolved material (e.g. precipitate, surface film, etc):no information
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture: internal culture
- Method of cultivation: on agar gel in test tubes
- Preparation of inoculum for exposure: the cultures are rinsed from the Agar gel using NaCl solution.
- Pretreatment:
- Initial biomass concentration: so that when mixed in a 1+ 9 dilution with NaCl, the extinction value is 10 times higher than that of a Formazin Standard suspension.
Study design
- Test type:
- static
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 16 h
- Post exposure observation period:
- none
Test conditions
- Test temperature:
- 25 °C
- pH:
- neutral
- Dissolved oxygen:
- no data
- Salinity:
- no data
- Nominal and measured concentrations:
- from the stock solution, dilutions of 2 exp 0 to 2 epx 14 are performed.
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: 160 mL
- Aeration: none, the test containers are closed with artificial material.
No additional data on test organisms number
OTHER TEST CONDITIONS
- Adjustment of pH: yes
- Photoperiod:
- Light intensity:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Cell increase reduction is measured through primary light absorption after correction with the dissolved substance absorption and protection from diffuse reflexion. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- 18 other: mg arsenate/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- 4.18 other: mg As/L
- Nominal / measured:
- nominal
- Basis for effect:
- growth inhibition
- Details on results:
- no
- Results with reference substance (positive control):
- no reference substance
Any other information on results incl. tables
For the determination of the effect concentration, the following procedure is used:
A halflogarith graph is used, with on X the substance concentrations and on Y the extinction values.
Calculation is made of the mean value of the extinction for test concentrations at which the cultures where non affected (neither stimulation nor inhibition) This is the point A . The allowed standard deviation is 3%. The mean value of the extinction in the three test cultures of the lowest toxic substance concentration is calculated. This is the point B. The 3 points A, B, and A-3 % are reported on a halflogarithm Y ax. The corresponding concentrations are a, b, and c. "c" is the concentration that corresponds to the beginning of the cell increase reduction.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Remarks:
- not applicable as the assessment of effects is not made theough gaz consumption
- Conclusions:
- Under the conditions of the test, the test substance showed a EC3% of 18 mg/L.
- Executive summary:
The toxicity of the substance di-sodiumhydrogenarsenate was tested on the bacteria pseudomonas putida. The organisms were exposed to different concentrations of the test substance, with dilution factors from 20to214.The inhibitory effect of the test substance on the organisms was measured through turbidimetric measurement. Cell increase reduction is measured through primary light absorption after correction with the dissolved substance absorption and protection from diffuse reflexion. A graphic assessment of the results was performed in order to derive the concentration at which the test substance starts reduce the cell number increase, i.e. when effects of more than 3% are observed.
The resulting EC3% is 18 mg/L for the tested substance.
This corresponds to 6.75 mg/L As element. (Calculation of the assessor).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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