Registration Dossier
Registration Dossier
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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 231-901-9 | CAS number: 7778-39-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Dermal absorption
Administrative data
- Endpoint:
- dermal absorption in vitro / ex vivo
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Study period:
- 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, meets generally accepted scientific principles, acceptable for assessment
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 994
Materials and methods
- Principles of method if other than guideline:
- In Vitro percutaneous absorption study was conducted to determine the dermal absorption of sodium arsenate (73As) in various donor vehicles (aqueous and solid) on the skin surface of female B6C3F1 mice under various conditions of exposure. Doses of 5, 50, 500 or 5000 ng were applied to the skin surface (area = 0.64 cm2) as the solid compound, in aqueous vehicle (100 and 250 µL) or in soil (23 mg/cm2). This experiment was conducted for 24 hours using previously clipped full-thickness dorsal skin in a flow-through system with HEPES-buffered Hanks' balanced salt solution as the receptor fluid. Dermal absorption was quantified by summing the amounts of arsenate-derived radioactivity using a gamma counter in the receptor fluid and skin following washing of the skin surface to remove unpenetrated compound.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Disodium hydrogenarsenate
- EC Number:
- 231-902-4
- EC Name:
- Disodium hydrogenarsenate
- Cas Number:
- 7778-43-0
- IUPAC Name:
- disodium hydrogen arsenate
- Details on test material:
- - Name of test material (as cited in study report): Sodium arsenate (73As)
- Source: Los Alamos National Laboratory, USA
- Radiochemical purity (if radiolabelling): >99%
Constituent 1
- Radiolabelling:
- yes
Test animals
- Species:
- mouse
- Strain:
- B6C3F1
- Sex:
- female
Administration / exposure
- Vehicle:
- water
- Duration of exposure:
- - 24 hours exposure for 5, 50, 500 or 5000 ng sodium arsenate (all vehicles)
- 1 hour exposure for 50 ng sodium arsenate (100 µL aqueous vehicle)
- 1 hour exposure for 50 ng sodium arsenate (100 µL aqueous vehicle) and receptor fluid perfused for additional 23 hours - Doses:
- 5, 50, 500 or 5000 ng
- No. of animals per group:
- Not applicable
Results and discussion
Percutaneous absorptionopen allclose all
- Dose:
- 100 µL aqueous vehicle
- Parameter:
- percentage
- Absorption:
- 62 %
- Remarks on result:
- other: 24 hours
- Remarks:
- maximum absorption (62% of the applied dose) was obtained from 100 µL aqueous vehicle and skin contained a higher level of the compound than the receptor fluid.
- Dose:
- Solid vehicle
- Parameter:
- percentage
- Absorption:
- < 0.3 %
- Remarks on result:
- other: 24 hours
- Remarks:
- Solid provided the least (<0.3% of the applied dose) absorption of the chemical, with the major portion (68%) of the absorbed dose residing within the skin
- Conversion factor human vs. animal skin:
- None
Any other information on results incl. tables
- Absorption of sodium arsenate increased linearly in all exposure vehicles, with a constant fraction of the dose being absorbed.
-
In aqueous vehicle (100 µL), maximum absorption (62% of the applied
dose) was obtained and the skin contained a higher level of the compound
than the receptor fluid.
- Solid provided the least (<0.3% of the applied dose) absorption of the
chemical, with the major portion (68%) of the absorbed dose residing
within the skin
- Skin compartment contained higher percentages of the permeated dose than the receptor fluid in all cases with the highest value (> 89% of permeated dose) was obtained with 250 µL aqueous vehicle
- Short term (1 hour) dermal exposure to arsenate in water resulted in the passage of the chemical into the skin, which on further perfusion (23 hours) passed into receptor fluid
Table 1:Maximum permeation rates of sodium arsenate through mouse skin from different exposure vehicles
Vehicle |
Applied dose (ng) |
Permeation rate* |
Water (100 µL) |
5 |
10.1 (4.8) |
50 |
9.1 (5.5) |
|
500 |
11.5 (2.7) |
|
Water (250 µL) |
5 |
0.9 (0.3) |
50 |
1.0 (0.1) |
|
500 |
1.9 (0.8) |
|
Solid |
5 |
4.9 (3.6) |
50 |
5.5 (2.7) |
|
500 |
5.5 (2.9) |
Percentage of applied dose/4 hours.
Values are mean ± SD of atleast five determinations
Applicant's summary and conclusion
- Conclusions:
- Under the test conditions, in vitro dermal absorption of sodium arsenate is greater in aqueous vehicle than soil in skin of B6C3F1mice
- Executive summary:
In vitro percutaneous absorption study was conducted to determine the dermal absorption of sodium arsenate (73As) in various donor vehicles (aqueous and solid) on the skin surface of female B6C3F1 mice under various conditions of exposure. Doses of 5, 50, 500 or 5000 ng were applied to the skin surface (area = 0.64 cm2) as the solid compound, in aqueous vehicle (100 and 250 µL) or in soil (23 mg/cm2). This experiment was conducted for 24 hours using previously clipped full-thickness dorsal skin in a flow-through system with HEPES-buffered Hanks' balanced salt solution as the receptor fluid. Dermal absorption was quantified by summing the amounts of arsenate-derived radioactivity using a gamma counter in the receptor fluid and skin following washing of the skin surface to remove unpenetrated compound.
Absorption of sodium arsenate increased linearly in all exposure vehicles, with a constant fraction of the dose being absorbed. In 100 µL aqueous vehicle, maximum absorption (62% of the applied dose) was obtained and the skin contained a higher level of the compound than the receptor fluid. While, solid provided the least (<0.3% of the applied dose) absorption and the major portion (68%) of the absorbed dose residing within the skin.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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