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Diss Factsheets
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EC number: 202-849-4 | CAS number: 100-41-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Acute Toxicity: other routes
Administrative data
- Endpoint:
- acute toxicity: other routes
- Adequacy of study:
- supporting study
- Study period:
- 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Experimental investigation, but study well documented, meeting generally accepted scientific principles, acceptable for assessment
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- publication
- Title:
- Structure-acute toxicity relationship of aromatic hydrocarbons in mice.
- Author:
- Tanii, H.; Huang, J.; Hashimoto, K.
- Year:
- 1 995
- Bibliographic source:
- Toxicol. Lett. 76:27-31.
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other:
- Principles of method if other than guideline:
- This study was carried out to assess the effect of metabolism on acute toxicity of aromatic hydrocarbons. To achieve this mice were pretreated with carbon tetrachloride at a dose level which did not cause death but which was known to inactivate hepatic microsomal monooxygenases
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Ethylbenzene
- EC Number:
- 202-849-4
- EC Name:
- Ethylbenzene
- Cas Number:
- 100-41-4
- Molecular formula:
- C8H10
- IUPAC Name:
- ethylbenzene
- Details on test material:
- - Name of test material (as cited in study report): Ethylbenzene.
The aromatic hydrocarbons evaluated in this structure -activity study were benzene, toluene, o, m and p-xylene, cumene, propyl and butyl benzene.
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- other: ddY mice
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- No data
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- other: Olive oil
- Details on exposure:
- The intraperitoneal LD50 was determined in mice for benzene and a series of 8 alkyl benzenes including ethyl benzene. The mice received either olive oil alone or olive oil containing 20% CCl4 by intraperitoneal injection 24 hours prior to determination of the intraperitoneal LD50 of the test materials. Observation continued over the following week and the test animals were observed thrice daily.
Additionally the n-octanol/water partition coefficient was measured for all the test materials - Doses:
- No data
- No. of animals per sex per dose:
- Groups of 4 mice were used at each of 4 dose levels.
- Control animals:
- not specified
- Details on study design:
- No data
- Statistics:
- The LD50 was calculated according to Weil, 1952. The structure activity relationship was analysed by the model of Hansch and Fujita using log P.
Results and discussion
Effect levels
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 17.81 other: mmol/kg
- Based on:
- test mat.
- Remarks on result:
- other: IP LD50 CCl4-pretreated = 17.81 mmol/kg (17.81 df = 0)
- Mortality:
- No data
- Clinical signs:
- No data
- Body weight:
- No data
- Gross pathology:
- No data
- Other findings:
- No data
Any other information on results incl. tables
None
Applicant's summary and conclusion
- Conclusions:
- IP LD50 control = 19.7 mmol/kg (range 16.09-24.14)
IP LD50 CCl4-pretreated = 17.81 mmol/kg (17.81 df = 0)
(attributed to reduced metabolism due to carbon tetrachloride poisoning)
The authors calculated the to demonstrate the effect of metabolism on acute toxicity. A ratio of <1 implies a substance becomes more toxic after metabolism. Correlation with log P revealed a rough relationship between toxicity following metabolic saturation and increasing hydrophobicity.
Results for ethyl benzene are presented together with those at each end of the spectrum of values obtained. This study showed a significant correlation between acute toxicity and hydrophobicity.
benzene LD50 cont/LD50 CCl4 = 1.35; log P = 2.24
ethyl benzene LD50 cont/LD50 CCl4 = 1.11; log P = 3.34
butyl benzene LD50 cont/LD50 CCl4 = 0.53; log P = 4.34 - Executive summary:
This study was carried out to assess the effect of metabolism on acute toxicity of aromatic hydrocarbons. To achieve this mice were pretreated with carbon tetrachloride at a dose level which did not cause death but which was known to inactivate hepatic microsomal monooxygenases.
The intraperitoneal LD50 was determined in mice for benzene and a series of 8 alkyl benzenes including ethyl benzene. The mice received either olive oil alone or olive oil containing 20% CCl4 by intraperitoneal injection 24 hours prior to determination of the intraperitoneal LD50 of the test materials. Observation continued over the following week and the test animals were observed thrice daily. Groups of 4 mice were used at each of 4 dose levels. The LD50 was calculated according to Weil, 1952.
Additionally the n-octanol/water partition coefficient was measured for all the test materials.
The structure activity relationship was analysed by the model of Hansch and Fujita using log P.IP LD50 control = 19.7 mmol/kg (range 16.09-24.14)
IP LD50 CCl4-pretreated = 17.81 mmol/kg (17.81 df = 0)
(attributed to reduced metabolism due to carbon tetrachloride poisoning)
The authors calculated to demonstrate the effect of metabolism on acute toxicity. A ratio of <1 implies a substance becomes more toxic after metabolism. Correlation with log P revealed a rough relationship between toxicity following metabolic saturation and increasing hydrophobicity.
Results for ethyl benzene are presented together with those at each end of the spectrum of values obtained. This study showed a significant correlation between acute toxicity and hydrophobicity.
benzene LD50 cont/LD50 CCl4 = 1.35; log P = 2.24
ethyl benzene LD50 cont/LD50 CCl4 = 1.11; log P = 3.34
butyl benzene LD50 cont/LD50 CCl4 = 0.53; log P = 4.34
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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