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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 201-983-0 | CAS number: 90-30-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well documented publication which meets basic scientific principles
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- determination of cytotoxicity measured as growth inhibition
- GLP compliance:
- no
- Analytical monitoring:
- no
- Details on sampling:
- - Concentrations: 0.1, 1, 10, 100 and 1000 µg/mL
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: dilution in acetone or water (stock solutions at 100 mg/mL), serial dilutions at 10-fold decrements from 100 mg/mL to 0.01 mg/mL (freshly made for each assay)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone or water (not further specified) - Test organisms (species):
- Tetrahymena pyriformis
- Details on inoculum:
- - Laboratory culture: yes
- Preparation of inoculum for exposure: maintained at 28°C, stock cultures were subcultured daily, inocula from 3-d old cultures (containing ca. 6 x 10e5 cells/mL) were diluted with sterile medium
- Initial biomass concentration: 1 x 10e4 cells/mL, diluted to final concentrations in the test tubes of 2 x 10e3 cells/mL - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 2 d
- Post exposure observation period:
- no
- Hardness:
- no data
- Test temperature:
- 28 °C
- pH:
- no data
- Dissolved oxygen:
- no data
- Salinity:
- no data
- Nominal and measured concentrations:
- 0.1, 1, 10, 100 and 1000 µg/mL (nominal conc.)
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: screw-capped Pyrex tubes (125 x 20 mm), 5mL volume
- Aeration: no
- No. of organisms per vessel: 2 x 10e3 cells/mL
- No. of vessels per concentration (replicates): min. 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 1.5% w/v Proteose peptone no. 3 (Difco) and 0.15% w/v yeast hydrolysate (Nutritional Biochemicals Corp.) with volatile preservative added (chlorobenzen 1 vol., 1,2-dichloroethane 1 vol., 1-chlorobutane 2 vols.), pH adjusted to 6.8
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Photoperiod: in the dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : cytotoxicity (microscopical examination of growth; classification as 100%, 50%, 25%, 12.5%)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10 - Reference substance (positive control):
- not specified
- Duration:
- 2 d
- Dose descriptor:
- IC50
- Effect conc.:
- 2 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- growth inhibition
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05.12.1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- no
- Analytical monitoring:
- no
- Details on sampling:
- - Concentrations: 0, 100, 1000 and 10000 mg/L
- Vehicle:
- not specified
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Laboratory culture: yes
- Method of cultivation: 3L laboratory (OECD)
- Pretreatment: no
- Initial biomass concentration: 6 g/L (dry wt) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- no data
- Test temperature:
- 19.7-20.6 °C
- pH:
- 7.4-7.9
- Dissolved oxygen:
- no data
- Salinity:
- no data
- Nominal and measured concentrations:
- no data
- Details on test conditions:
- TEST SYSTEM
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Total volume in test vessels: 250 mL
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic nutrient solution (peptone 16 g/L, meat extract 11 g/L, NH2-CO-NH2 3 g/L, NaCl 0.7 g/L, CaCl2*2H2O 0.4 g/L, MgSO4*7H2O 0.2 g/L, K2HPO4 2.8 g/L)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): activated sludge respiration rate
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorphenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - Results with reference substance valid: yes
Referenceopen allclose all
Respiration rate [mg/L*h] Inhibition [%] Test substance 100 mg/L 33 0 Test substance 1000 mg/L 27 5 Test substance 10000 mg/L 24 16 Reference substance 1 mg/L 24 16 Reference substance 20 mg/L 6 79 Negative control 28.5 - Autoxidation control 0 0
Description of key information
EC50(3h) > 10000 mg/L (nominal) for activated sludge (OECD 209)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 10 000 mg/L
Additional information
One study investigating the toxicity of the test substance to aquatic microorganisms is available. The study was performed according to OECD guideline 209 using non-adapted activated sludge as inoculum (Kanne 1989). Nominal concentrations of 0, 100, 1000 and 10000 mg/L were tested, and an EC50 value (3h) of > 10000 mg/L was obtained for the inhibition of the respiration rate.
Another study reports on the toxicity of Neozone A (a mixture containing N-phenyl-1-naphtylamine, not further specified) to Tetrahymena pyrifomis, yielding an IC50 value (2d) of 2 mg/L for growth inhibition, following no guideline.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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