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Diss Factsheets
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EC number: 202-974-4 | CAS number: 101-77-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The stock solution (4.6 mg/L) was prepared by directly adding 9.23 mg of test substance to 2L of test medium. The mixture was placed in an ultrasonic bath for 10 minutes then stirred for about 1 day at 20 ± 2 °C. After stirring the stock solution was clear and colorless with no visible undissolved test substance. However, by request of the sponsor, the stock solution was centrifuged (approx. 20 min at about 17700 G with a Beckman CoulterTM, Avanti J-25) to assure the removal of any undissolved test substance. The lower test concentrations were prepared by dilution of this stock solution. Before the dilution was performed, the stock solution was checked for complete dissolution of the test substance.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Ecotoxicology Laboratory of Experimental Toxicology and Ecology, BASF SE, Ludwigshafen Germany.
- Age at study initiation (mean and range, SD): < 24 hours (at least 3rd brood progeny)
- Feeding during test: none
ACCLIMATION
- Acclimation period: no data
- Acclimation conditions (same as test or not): yes
- Type and amount of food: unicellular algae (Desmodesmus subspicatus) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 2.20 – 3.20 mmol/L
- Test temperature:
- 20 ± 2 °C.
- pH:
- 7.8-7.9
- Dissolved oxygen:
- 8.6-9.0 mg/L
- Details on test conditions:
- 0, 0.1, 0.22, 0.46, 1.0, 2.2 and 4.6 mg/L (nominal)
0, 0.09, 0.19, 0.41, 0.91, 2.0 and 4.3 mg/L (mean measured) - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 0.19 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.35 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL = 0.18-0.71 mg/L
- Details on results:
- - Effect concentrations exceeding solubility of substance in test medium: no
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50/LC50: The EC50(24h) of the reference substance potassium dichromate was 1.04 mg/L (experiment date: 09 Dec 2010, project number: 50E0063/043039). This result is within the range of 0.6 – 2.1 mg/L (ISO 6341) and indicates that the culture of Daphnia magna used in this study is responding normally to toxic stress. - Validity criteria fulfilled:
- yes
- Conclusions:
- Acutely very toxic to aquatic invertebrates.
Reference
Description of key information
After 48 hours of exposure an EC50 of 0.35 mg/L was estimated (BASF, 2011), related to the mean measured concentration. This result indicates that the substance is acutely very toxic to aquatic invertebrates.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 0.35 mg/L
Additional information
Acute toxicity to aquatic invertebrates was investigates in a static approach according to GLP and OECD guideline 202 using Daphnia magna as test organism (BASF 2011). After 48 hours of exposure an EC50 of 0.35 mg/L was estimated, related to the mean measured concentration. This result indicates that the substance is acutely very toxic to aquatic invertebrates. In a semi-static approach according to the same guideline and with the same test species a slightly higher EC50 of 2.47 mg/L was determined (Mitsubishi, 2002). The more sensitive value of 0.35 mg/L was used for GHS classification and PNEC derivation.
Two non GLP studies according to OECD 202 assessed the aquatic toxicity to Moina macrocopa (EC50 (24h) = 2.3 ppm) (Fujiwara, 1982) and Daphnia magna (EC100(24h) >100 mg/L) (Rulfi & Mueller, 1986). Due to methodological and documentary deficiencies theses studies were rate less reliable than the above-mentioned studies of Mitsubishi and BASF. Nevertheless, these information can be considered as additional information.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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