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Diss Factsheets
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EC number: 204-402-9 | CAS number: 120-51-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 31 January 1985 to 26 February 1985
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The test material was probably administered, at various concentrations in peanut oil, as the technical grade material but no information was available to confirm purity.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- Principles of method if other than guideline:
- Standard limit test according to OECD 401
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Benzyl benzoate
- EC Number:
- 204-402-9
- EC Name:
- Benzyl benzoate
- Cas Number:
- 120-51-4
- Molecular formula:
- C14H12O2
- IUPAC Name:
- benzyl benzoate
- Test material form:
- other: clear colourless liquid
- Details on test material:
- - Name of test material (as cited in study report): benzylbenzoat H&R or benzylbenzoate
- clear, colourless liquid
- Analytical purity: no data
- Lot/batch No.: 4128806 B
- Expiration date of the lot/batch: no data
stored at room temperature
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: A Tuck & Sons Ltd, Essex, UK
- Age at study initiation: 4-6 weeks old
- Weight at study initiation: 119-148g males and 118-148g females
- Fasting period before study: yes, overnight unti 2 hours aftre dosing
- Housing: groups of five by se in polypropylene cages
- Diet (e.g. ad libitum):Rat & Mouse Expanded No.1, supplied by SDS Witham, Essex; ad libitum
- Water (e.g. ad libitum): ad libitum access to tap water
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-24
- Humidity (%): 40-55%
- Air changes (per hr): 10
- Photoperiod (hrs dark / hrs light): 12/12
IN-LIFE DATES: From: 31 January 1985 To: 26 February 1985
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- arachis oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: For range finding test - 20, 100, 200, 300 and 400 mg/ml and for the main study 400 mg/ml
- Amount of vehicle (if gavage): dose volume was 5 mL/kg bw
- Justification for choice of vehicle: arachis oil used basd on suitability during range-finding investigations
MAXIMUM DOSE VOLUME APPLIED: 5 mL/kg
DOSAGE PREPARATION (if unusual): prepared freshly for each dose administration - Doses:
- range-finder: 100, 500, 1000, 1500 and 2000 mg/kg bw
main study: 2000 mg/kg bw - No. of animals per sex per dose:
- 2 in range finder; 5 in main study
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: frequent hourly observations on day of dosing; daily checks thereafter; bodyweights recorded on days 0, 7 and 14
- Necropsy of survivors performed: yes - Statistics:
- Not required
Results and discussion
- Preliminary study:
- The range finding investigation revealed no mortalities in the dose range 100 to 2000 mg/kg bw. Hunched posture, lethargy, decreased respiratory rate and piloerection were frequently observed in the range-finder groups but the signs and severity of clinical response showed no treatment relationship. Recovery from systemic effects was complete within three days for all groups.
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: No deaths at the limit dose
- Mortality:
- There were no deaths in the main study, following dosing at 2000 mg/kg bw.
- Clinical signs:
- other: Hunched posture, lethargy, piloerection, ptosis and a decreased respiratory rate were commonly observed shortly after dosing with infrequent cases of body tremors also noted. Recovery from all signs was complete by Day 4.
- Gross pathology:
- Macroscopic examination at necropsy revealed congested lungs for one rat (normally an agonal response) and pale areas on the gastric glandular region for one rat. No other macroscopic abnormalities were apparent.
- Other findings:
- No data
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The acute oral LD50 for benzylbenzoate was greater than 2000 mg/kg bw in rats.
- Executive summary:
In a standard acute oral study in rats, in accordance with test guideline OECD 401, groups of five male and female rats were dosed at 2000 mg/kg bw. Clinical signs including hunched posture, decreased respiratory rate, lethargy, ptosis and pilo-erection were apparent shortly after dosing but recovered within 4 days. The acute oral median lethal dose for benzylbenzoate was greater than 2000 mg/kg bw in rats.
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