Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-402-9 | CAS number: 120-51-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 03/01/2013 - 11/03/2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.20 (Daphnia magna Reproduction Test)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken from new and old media when the media were being renewed (3 days per week).
- Vehicle:
- no
- Details on test solutions:
- A stock solution was created by adding 20mg of the test substance to 2 litres of pH adjusted dilution water and treating with an ultra-turrax for 60 seconds at 8000 rpm and stirred for 10 minutes with a magnetic stirrer. The pH was adjusted to 7.0. To create the test concentrations appropriate amounts of the stock solution were diluted with pH adjusted dilution water to a volume of 2000mL. For each replicate 100 mL of these solutions was taken. Ten replicates were prepared for each test concentration.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Test organisms were parthenogenetic female Daphnia magna STRAUS. They were sourced from an in-house population which is maintained at constant temperature (20±1oC) with a 16 hour light/8 hour dark photoperiod. Daphnia are fed exclusively with unicellular green algae (Desmodesmus subspicatus).
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 15.8-16.8 dH
- Test temperature:
- 19.8-20.3oC
- pH:
- 7.0-7.7
- Dissolved oxygen:
- 8.5-9.9
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations: 0(control), 0.125, 0.25, 0.5, 1, 2, and 3 mg/L
- Details on test conditions:
- Following two range-finding test Daphnia were exposed to the test substance at nominal concentrations of 0.125, 0.25, 0.5, 1, and 3 mg/L. Single Daphnia neonates were placed in 150mL beakers with 100 mL of test solution. Ten replicates were used for each concentration. During the test the test solutions were renewed three times per week. The incubation temperature during the test was 19.8-20.3oC, with a photoperiod of 16h light: 8h dark, and a light intensity not exceeding 20 µE x m-2 x s-1.
Daphnia were fed daily with living cells of Desmodesmus subspicatus.
The criterion of adverse effects used in this study was the item-induced inhibitory effect on the parthenogenetic reproduction rate and the mobility behaviour/mortality rate of parent Daphnia, all effects being assessed at least three times a week. However, test vessels were inspected daily for the occurrence of juveniles and marked accordingly. When juveniles in these marked vessels were counted the next day (or after the weekend), the number of offspring was assigned to the previous day when juveniles were first observed. - Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.258 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.455 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.97 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 0.97 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- Concentrations in the test solutions ranged from 81.0% to 92.1% of the nominal values in the freshly prepared media and from 1.0% to 46..8% in the media after 48 or 72 hours exposure. As such, endpoints were calculated based on time weighted average values.
The 21-day NOEC for reproduction was calculated to be 0.258 mg/L , and the LOEC was calculated to be 0.455 mg/L.
For mortality the 21-day NOEC was calculated to be ≥ 0.97 mg/L, and the LOEC > 0.97 mg/L. - Reported statistics and error estimates:
- The reproductive output of the Daphnia exposed to the test item was compared to that of the control in order to determine the Lowest Observed Effect Concentration (LOEC) and the No Observed Effect Concentration (NOEC) according to Williams Multiple Sequential t-Test Procedure and to Fisher`s Test with Bonferroni Correction.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 21-day NOEC for reproduction was determined to be 0.258 and the 21-day LOEC for reproduction was determined to be 0.455 mg/L.
- Executive summary:
A 21 -day test was conducted to assess the effects of benzyl benzoate on the reproductive output of parthenogenetic Daphnia magna under semi static conditions. Female Daphnia magna neonates were exposed to the test substance at concentrations of 0.125, 0.25, 0.5, 2, and 3 mg/L. Test organisms were kept under constant light and temperature, and test media was renewed 3 times per week. The number of offspring produced was recorded throughout the test. The number of mortalities was also recorded.
After 21 -days exposure, the total number of living offspring produced per parent animal alive at the end of the test was assessed.
The 21 -day NOEC for reproduction was determined to be 0.258 mg/L based on time-weighted average concentrations. The 21 -day LOEC for reproduction was determined to be 0.455 mg/L based on time-weighted average concentrations.
Reference
Table 1: Mean reproduction rate
Test item concentration |
Time-weighted mean measured concentration [mg/L] |
Mean reproduction rate |
SD |
CV [%] |
Control |
---- |
95.3 [10] |
9.7 |
10.2 |
0.125 |
----- |
97.1 [10] |
9.5 |
9.8 |
0.25 |
----- |
102.3 [9] |
9.8 |
9.5 |
0.5 |
0.123 |
98.7 [9] |
21.8 |
22.1 |
1 |
0.258 |
91.5 [10] |
20.3 |
22.1 |
2 |
0.455 |
69.6 [9] |
22.9 |
32.9 |
3 |
0.970 |
29.1 [8] |
17.1 |
58.5 |
Table 18- Immobilisation/mortality rate and mean reproduction rate of parent Daphnia
Test item nominal |
Time-weighted mean measured concentration [mg/L] |
Immobilisation/ |
Mean reproduction rate of parentDaphnia |
||
[mg/L] |
|
absolute |
% |
absolute |
% |
Control |
---- |
0 |
0 |
95.3 [10] |
100 |
0.125 |
----- |
0 |
0 |
97.1 [10] |
101.9 |
0.25 |
----- |
1 |
10 |
102.3 [9] |
107.3 |
0.5 |
0.123 |
1 |
10 |
98.7 [9] |
103.6 |
1 |
0.258 |
0 |
0 |
91.5 [10] |
96.0 |
2 |
0.455 |
1 |
10 |
69.6 [9] |
73.0 |
3 |
0.970 |
2 |
20 |
29.1 [8] |
30.5 |
Table 8- Abiotic parameters of control
Day |
Date 2013 |
Parameter |
Replicate 1 (new medium) |
Day |
Date 2013 |
Parameter |
Replicate 1 (old medium) |
0 |
01-16 |
pH value |
7.2 |
2 |
01-18 |
pH value |
7.7 |
0 |
01-16 |
O2[mg/L] |
8.8 |
2 |
01-18 |
O2[mg/L] |
8.9 |
0 |
01-16 |
O2[%] |
98 |
2 |
01-18 |
O2[%] |
100 |
0 |
01-16 |
t [°C] |
20.9 |
2 |
01-18 |
t [°C] |
20.1 |
0 |
01-16 |
[°dH] |
16.2 |
2 |
01-18 |
[°dH] |
16.4 |
7 |
01-23 |
pH value |
7.1 |
9 |
01-25 |
pH value |
7.3 |
7 |
01-23 |
O2[mg/L] |
8.7 |
9 |
01-25 |
O2[mg/L] |
9.4 |
7 |
01-23 |
O2[%] |
97 |
9 |
01-25 |
O2[%] |
101 |
7 |
01-23 |
t [°C] |
20.8 |
9 |
01-25 |
t [°C] |
19.8 |
7 |
01-23 |
[°dH] |
16.1 |
9 |
01-25 |
[°dH] |
16.5 |
16 |
02-01 |
pH value |
7.0 |
19 |
02-04 |
pH value |
7.3 |
16 |
02-01 |
O2[mg/L] |
8.6 |
19 |
02-04 |
O2[mg/L] |
8.8 |
16 |
02-01 |
O2[%] |
96 |
19 |
02-04 |
O2[%] |
95 |
16 |
02-01 |
t [°C] |
20.8 |
19 |
02-04 |
t [°C] |
19.8 |
16 |
02-01 |
[°dH] |
15.8 |
19 |
02-04 |
[°dH] |
16.1 |
Table 9- Abiotic parameters 3mg/L (nominal)
Day |
Date 2013 |
Parameter |
Replicate 1 (new medium) |
Day |
Date 2013 |
Parameter |
Replicate 1 (old medium) |
0 |
01-16 |
pH value |
7.2 |
2 |
01-18 |
pH value |
7.6 |
0 |
01-16 |
O2[mg/L] |
8.6 |
2 |
01-18 |
O2[mg/L] |
8.7 |
0 |
01-16 |
O2[%] |
99 |
2 |
01-18 |
O2[%] |
96 |
0 |
01-16 |
t [°C] |
21.1 |
2 |
01-18 |
t [°C] |
20.3 |
0 |
01-16 |
[°dH] |
16.2 |
2 |
01-18 |
[°dH] |
16.3 |
7 |
01-23 |
pH value |
7.1 |
9 |
01-25 |
pH value |
7.6 |
7 |
01-23 |
O2[mg/L] |
8.7 |
9 |
01-25 |
O2[mg/L] |
9.9 |
7 |
01-23 |
O2[%] |
98 |
9 |
01-25 |
O2[%] |
105 |
7 |
01-23 |
t [°C] |
21.0 |
9 |
01-25 |
t [°C] |
19.4 |
7 |
01-23 |
[°dH] |
16.8 |
9 |
01-25 |
[°dH] |
16.4 |
16 |
02-01 |
pH value |
7.0 |
19 |
02-04 |
pH value |
7.5 |
16 |
02-01 |
O2[mg/L] |
8.5 |
19 |
02-04 |
O2[mg/L] |
8.9 |
16 |
02-01 |
O2[%] |
97 |
19 |
02-04 |
O2[%] |
97 |
16 |
02-01 |
t [°C] |
21.0 |
19 |
02-04 |
t [°C] |
19.7 |
16 |
02-01 |
[°dH] |
15.8 |
19 |
02-04 |
[°dH] |
16.1 |
Description of key information
The 21-day NOEC for reproduction in Daphnia magna was determined to be 0.258 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- NOEC
- Effect concentration:
- 0.258 mg/L
Additional information
A 21 -day test was conducted to assess the effects of benzyl benzoate on the reproductive output of parthenogenetic Daphnia magna under semi static conditions. Female Daphnia magna neonates were exposed to the test substance at concentrations of 0.125, 0.25, 0.5, 2, and 3 mg/L. Test organisms were kept under constant light and temperature, and test media was renewed 3 times per week. The number of offspring produced was recorded throughout the test. The number of mortalities was also recorded.
After 21 -days exposure, the total number of living offspring produced per parent animal alive at the end of the test was assessed.
The 21 -day NOEC for reproduction was determined to be 0.258 mg/L based on time-weighted average concentrations. The 21 -day LOEC for reproduction was determined to be 0.455 mg/L based on time-weighted average concentrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.