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Diss Factsheets
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EC number: 204-402-9 | CAS number: 120-51-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23/6/1992 - 21/7/1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- This study was carried out according to EU guideline C.4-D and GLP standards were maintained throughout.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- The inoculum was an activated sludge taken from a laboratory activated sludge facility fed with municipal waste water. The sample was taken on 22/6/1992.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- A weighed amount of the test substance was added to mineral medium to create a test concentration of 100 mg/l. The test media was inoculated with activated sludge and incubated for 28 days under aerobic conditions. Test temperature was 20± 1°C. During this time the oxygen consumption in the test vessels was measured with a respirometer. The rate of oxygen consumption was used to determine the biological degradation of the test substance.
- Reference substance:
- aniline
- Preliminary study:
- No information provided
- Test performance:
- All validity criteria for the test were met and the eference substance was 75% degraded within 28 days.
- Parameter:
- % degradation (O2 consumption)
- Value:
- 94
- Sampling time:
- 28 d
- Details on results:
- The test substance was found to be 94% degraded within 28 days and is thus considered to be readily biodegradable.
- Results with reference substance:
- The reference substance (aniline) was 75% degraded within 28 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test substance was 94% degraded after 28 days and is therefore considered readily biodegradable.
- Executive summary:
A 28 -day biodegradation study was carried out for benzyl benzoate according to EU method C.4 -D. The test substance was added to mineral medium to create a test concentration of 100 mg/l. Test solutions were inoculated with activated sludge and incubated under aerobic conditions for 28 days. Test temperature was 20 ± 1oC. The dissolved oxygen concentration was measured with a respirometer and the biodegradation of the substance was calculated from oxygen consumption. The test substance was found to be 94% degraded after 28 days. The 10-day window was met. Thus, the test substance is considered to be readily biodegradable.
Reference
Table 1: Oxygen consumption and calculated degradation results
Day | Oxygen consumption (mg/l) | Biological degradation in % | |
Test substance | Control | ||
2 | 101 | 3 | 41 |
4 | |||
6 | 195 | 9 | 77 |
8 | 208 | 11 | 82 |
10 | 217 | 11 | 85 |
12 | |||
14 | 226 | 12 | 89 |
16 | 231 | 13 | 90 |
18 | |||
20 | 234 | 14 | 91 |
22 | 235 | 14 | 92 |
24 | 239 | 15 | 93 |
26 | |||
28 | 242 | 15 | 94 |
Description of key information
Benzyl benzoate was found to be 94% degraded in an EU-method C.4 ready biodegradability test. As such, the substance is considered to be readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
A 28-day biodegradation study was carried out for benzyl benzoate according to EU method C.4 -D. The test substance was added to mineral medium to create a test concentration of 100 mg/l. Test solutions were inoculated with activated sludge and incubated under aerobic conditions for 28 days. Test temperature was 20± 1°C. The dissolved oxygen concentration was measured with a respirometer and the biodegradation of the substance was calculated from oxygen consumption. The test substance was found to be 94% degraded after 28 days. The 10-day window was met. Thus, the test substance is considered to be readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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