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Diss Factsheets
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EC number: 231-639-5 | CAS number: 7664-93-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Published, investigative, non-guideline, non-GLP study
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- A chronic toxicity study was performed with the aquatic midge Tanytarsus dissimilis to determine the effects on growth and reproduction in natural lake water with pH adjustment using sulphuric acid.
- GLP compliance:
- not specified
- Remarks:
- older, published study
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable - Analytical monitoring:
- yes
- Details on sampling:
- Not applicable
- Vehicle:
- no
- Details on test solutions:
- Test media were prepared using carbon-filtered Lake Superior water. The pH was adjusted using sulphuric acid
- Test organisms (species):
- other: Tanytarsus dissimilis
- Details on test organisms:
- The species was selected for use because of its widespread distribution, value as food for fish, ease of rearing in the laboratory and its relatively short life cycle.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Remarks on exposure duration:
- Three generations in the control
- Post exposure observation period:
- Not applicable
- Hardness:
- Test water data (after 35 days), all values in mg/L:
pH 3.0 - 78.0, pH 4.0 - 64.0, pH 5.0 - 54.0, pH 5.5 - 54.0, pH 6.0 - 50.0, pH 7.8 - 50.0 - Test temperature:
- Test water data (after 35 days), all values in degrees C:
pH 3.0, 4.0, 5.0, 5.5, 6.0 and 7.8 - all were 18.5 - pH:
- Not applicable
- Dissolved oxygen:
- Test water data (after 35 days), all values in mg/L:
pH 3.0 - 7.71, pH 4.0 - 8.06, pH 5.0 - 7.93, pH 5.5 - 8.70, pH 6.0 - 8.54, pH 7.8 - 9.01 - Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal pH values:
pH 3.0, pH 4.0, pH 5.0, pH 5.5, pH 6.0, pH 7.8 - Details on test conditions:
- A series of different pH were prepared by adjusting the pH of natural, carbon- filtered lake water with sulphuric acid. The following pHs were used:
pH 3.0, 4.0, 5.0, 5.5 and 6.0. Lake water was used as a control and had a pH of 7.8. - Reference substance (positive control):
- no
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.15 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: equivalent to pH 5.5
- Details on results:
- pH 3.0 - all larvae died within 5 days
pH 4.0 - all larvae died within 10 days
pH 5.0 - larvae survived, began pupating after 13 to 17 days, adults failed to emerge within 35 days. Five of the pupal cases were examined, larvae had successfully pupated and adult characteristics were present but the adults were unable to escape from the pupal case. The 10 remaining larvae were kept under test conditions for an additional 10 days and no adults appeared.
pH 5.5 - larve survived, began pupating after 12 to 14 days, adults began to emerge after 27 days, all 15 larvae emerged successfully within 35 day, eggs hatched within 35 days. The treatment was allowed to continue and three complete generations were obtained. The numbers increased from the original 15 to between 500 and 550 larvae.
pH 6.0 - larve survived, began pupating after 12 to 14 days, adults began to emerge after 27 days, all 15 larvae emerged successfully within 35 day, eggs hatched within 35 days. The treatment was allowed to continue and three complete generations were obtained. The numbers increased from the original 15 to between 500 and 550 larvae.
pH 7.8 (control) - larve survived, began pupating after 12 to 14 days, adults began to emerge after 27 days, all 15 larvae emerged successfully within 35 day, eggs hatched within 35 days. The treatment was allowed to continue and three complete generations were obtained. The numbers increased from the original 15 to between 500 and 550 larvae.
Based on the results an NOEC of pH 5.5 (equivalent to 0.15 mg/L) was observed. - Results with reference substance (positive control):
- not applicable
- Reported statistics and error estimates:
- not applicable
- Validity criteria fulfilled:
- not applicable
- Remarks:
- There were no appropriate study guidelines when the study was conducted.
- Conclusions:
- Based on the reported information an acceptable assessment of chronic toxicity to aquatic invertebrates was achieved.
- Executive summary:
A chronic toxicity study with the midge Tanytarsus dissimilis was conducted. Midge larvae were exposed to a series of test media, carbon-filtered lake water with pH adjustment using sulphuric acid, to varying pHs. At pH 3.0 and 4.0 all of the larvae died within 10 days. At pH 5 larvae pupated but the adults were unable to escape from the pupal case. At pH 5.5 and 6.0 there were no effects on the initial larvae and three complete generations were obtained. The initial 15 larvae increased to between 500 and 550 larvae. The results from the control group (lake water with no adjustment pH 7.8) were the same as in the pH 5.5 and 6.0.
The no-observed effect concentration (NOEC) for T. dissimilis (based on reproduction) exposed to sulphuric acid was observed to be pH 5.5, equivalent to 0.15 mg/L.
Reference
Only a brief description of the materials and methods was reported. However, the reported study details indicate that the study was conducted in a scientifically valid way and the results are considered to be reliable.
Description of key information
A published paper on the chronic effects of sulphuric acid on a freshwater invertebrate, the midge Tanytarsus dissimilis has been summarised and a chronic NOEC obtained.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- NOEC
- Effect concentration:
- 0.15 mg/L
Additional information
A chronic toxicity study with the midge Tanytarsus dissimilis was conducted. Midge larvae were exposed to a series of test media, carbon-filtered lake water with pH adjustment using sulphuric acid, to varying pHs. At pH 3.0 and 4.0 all of the larvae died within 10 days. At pH 5 larvae pupated but the adults were unable to escape from the pupal case. At pH 5.5 and 6.0 there were no effects on the initial larvae and three complete generations were obtained. The initial 15 larvae increased to between 500 and 550 larvae. The results from the control group (lake water with no adjustment pH 7.8) were the same as in the pH 5.5 and 6.0.
The no-observed effect concentration (NOEC) for T. dissimilis (based on reproduction) exposed to sulphuric acid was observed to be pH 5.5, equivalent to 0.15 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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