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EC number: 200-449-4 | CAS number: 60-00-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: EEC Guideline XI/681/86, Draft 4: "Prolonged toxicity study with Daphnia magna: Effects on reproduction"
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Principles of method if other than guideline:
- 21d semistatic long-term toxicity test assessing the effect of sublethal concentrations of the test substance on reproductive output of Daphnia magna
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Batch No.: 31-9412
- State of aggregation: powder
- Purity of active ingredient: > 89%
- Impurities / secondary components: see characterisation from ZAX-report No. 96L00703 - Analytical monitoring:
- yes
- Details on sampling:
- Samples for analysis were taken in the 1st, the 2nd and the 3rd week of the test. For each concentration the freshly prepared test solution (unstocked) and the corresponding 48 h or 72 h old test solution (stocked with daphnids) were analyzed.
- Vehicle:
- no
- Details on test solutions:
- At the beginning of the test and before changing the test solutions, a stock solution of the test substance was freshly prepared. A defined amount of the substance was weighed out and suspended directly in the test medium by stirring (about 10 min at 22 ± 1 °C). The nominal concentrations of the stock solutions were 100 mg/L. By dilution of the stock solutions with test medium, the nominal test concentrations were prepared.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: inhouse culture originally obtained from Institut National de Recherche Chimique Appliquée, France, 1978
- Age at study initiation (mean and range, SD): < 24h
- Feeding during test
- Food type: living green algae (D. subspicatus)
- Amount: days 0-1: 0.22 mg COD; days 2-3: 0.25 mg COD; days 4-5: 0.35 mg COD; days 6-7: 0.43 mg COD, days 8 -21: 0.75 mg COD
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 2.20 - 3.20 mmol/L
- Test temperature:
- 19.9 - 21.7 °C
- pH:
- 7.5-8.5
- Dissolved oxygen:
- 8.0 - 9.6 mg/L
- Conductivity:
- 550 - 650 µS/cm
- Nominal and measured concentrations:
- Nominal: 100; 50.0; 25.0; 12.5; 6.25; 3.13 and 1.56 mg/L
Measured: > 80% nominal - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL beaker containing 50 mL test solution
- Renewal rate of test solution (frequency/flow rate): three times per week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 medium
- Culture medium different from test medium: no
- Intervals of water quality measurement: Temperature was measured continuously in a separat beaker.DO and pH were recorded at every renewa lin the old and new test solution (in one beaker per concentration).
OTHER TEST CONDITIONS
- Photoperiod: 16h light: 8h dark
- Light intensity: 5-6 µE/(m2*s)
EFFECT PARAMETERS MEASURED: Reproductive output and mortality was recorded daily theroughout the test. - Reference substance (positive control):
- yes
- Remarks:
- 3,4-Dichloroaniline
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: NOEC = 2.5 µg/L - Reported statistics and error estimates:
- For the statistical evaluation of the LOEC and NOEC Duncan's new multiple range test was used.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance is not harmful to aquatic invertebrates at chronic exposure.
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Category justification document is attached in chapter 13.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Conclusions:
- The test substane is not harmful to Daphnia magna at chronic sublethal exposure.
Referenceopen allclose all
Analytically found relative
minimum and maximum concentrations of the test substance in the test
solutions at different times of the test (with and without daphnids).
The values are given in percent of the nominal concentrations:
Time [h] | stocked with daphnids | Min [%] | Max [% ] |
0 | no | 80.8 | 166.7 |
48 | no | 92.8 | 166.7 |
48 | yes | 95.2 | 173.1 |
M4 -water caused a
background value of about 1 mg/L of the test material. Regarding to this
and to the precision of the analytical method, most concentrations found
corresponded to the expected values.
Validity criteria
In the control:
- the mortality of parent animals up to the end of the test was <= 10 %.
- the mean number of living young per parent animal that survived the
test was >= 60.
- the coefficient of variation for the mean number of living young per
parent animal that survived the test was <= 25 %.
- The NOEC of the control substance 3,4-dichloroaniline was 2.5 µg/L,
the LOEC was 10 µg/L and LC0 was > 10 µg/L.
The validity criteria were fulfilled by the test.
Control:
In the control the first young were observed at day 7. In the
highest concentration tested, at which the daphnids produced young (100
mg/L), the first young were observed at day 13.
Description of key information
NOEC(21d) = 25 mg/L for reproduction of Daphnia magna (OECD 211, read across)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 25 mg/L
Additional information
Since no studies are available investigating the long-term toxicity of edetic acid (CAS No. 60-00-4) to aquatic invertebrates, the assessment was based on a study conducted with Subcategory 1 member Disodium dihydrogen ethylenediaminetetraacetate (CAS No. 139-33-3) in a read across approach. This is in accordance with Regulation (EC) No. 1907/2006, Annex XI, 1.5. Grouping of substance and read across approach. Further justification for suitability of read across between Subcategory 1 members is given within the category justification attached to chapter 13.
The key study was performed according to EEC Guideline XI%681/86, Draft 4 : "Prolonged toxicity study with Daphnia magna: Effects on reproduction", which is equivalent to OECD guideline 211
(BASF 1998). The semistatic study was conducted under GLP conditions using Daphnia magna as test organism (BASF 1998). Concentrations between 1.56 and 100 mg/L were tested. and analytically verified (> 80% of nominal). The 21d-NOEC value for reproductive output is determined to be 25 mg/L. Based on the suitability of the read across approach this conclusion can also considered to be true for edetic acid (CAS No. 60-00-4). Being the lowest NOEC value of all three trophic levels, this value is used for the derivation of PNECfreshwater and marine water.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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