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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 200-449-4 | CAS number: 60-00-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in soil
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in soil, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Laboratory study on biodegradation of EDTA in different agricultural soils over an incubation time of 4 to 45 weeks.
- GLP compliance:
- no
- Test type:
- laboratory
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: 4813
RADIOLABELLING INFORMATION (if applicable)
- Radiochemical purity: 99%
- Specific activity: 2.54 mCi/mM
- Locations of the label: 14C-carboxyl-EDTA - Radiolabelling:
- yes
- Oxygen conditions:
- aerobic
- Soil classification:
- not specified
- Year:
- 1 977
- Soil no.:
- #1
- Soil type:
- other: Brookston loam
- pH:
- 6.8
- Soil no.:
- #2
- Soil type:
- other: Conover sandy loam
- pH:
- 6.7
- Soil no.:
- #3
- Soil type:
- other: Spinks loamy fine sand
- pH:
- 6.4
- Soil no.:
- #4
- Soil type:
- other: Miami sandy loam
- pH:
- 6
- Details on soil characteristics:
- SOIL COLLECTION AND STORAGE
All soil samples were collected and stored in sealed plastic bags at 2 °C. All samples were taken in Michigan and each were a composite of subsamples collected at three locations within a 10 m2 area. - Soil No.:
- #1
- Duration:
- 4 - 45 wk
- Soil No.:
- #2
- Duration:
- 4 - 45 wk
- Soil No.:
- #3
- Duration:
- 4 - 15 wk
- Soil No.:
- #4
- Duration:
- 4 - 15 wk
- Soil No.:
- #1
- Initial conc.:
- 2.5 - 4.5 mg/kg soil d.w.
- Based on:
- test mat.
- Soil No.:
- #2
- Initial conc.:
- 2.5 - 4.5 mg/kg soil d.w.
- Based on:
- test mat.
- Soil No.:
- #3
- Initial conc.:
- 2.5 - 4.5 mg/kg soil d.w.
- Based on:
- test mat.
- Soil No.:
- #4
- Initial conc.:
- 2.5 - 4.5 mg/kg soil d.w.
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- radiochem. meas.
- Details on experimental conditions:
- 25 g fresh weight of soil was added to 125 mL Erlenmeyer flasks that had been fitted with rubber stoppers which supported a 2 mL plastic beaker containing 1 mL of 1N NaOH. The soils were adjusted to ~ field capacity. Microdrops of neutralized 14C-EDTA solutions were randomly distributed over the soil surface using a 28-gage needle on a 100 µL syringe. All soils were sealed and incubated at room temperature (25-27 °C). All of the reported data are means from duplicate or triplicate.
- Key result
- Soil No.:
- #1
- % Degr.:
- 65
- Parameter:
- CO2 evolution
- Remarks:
- [14CO2]
- Sampling time:
- 45 wk
- Key result
- Soil No.:
- #2
- % Degr.:
- 70.5
- Parameter:
- CO2 evolution
- Remarks:
- [14CO2]
- Sampling time:
- 45 wk
- Soil No.:
- #1
- % Degr.:
- 44.5
- Parameter:
- CO2 evolution
- Remarks:
- [14CO2]
- Sampling time:
- 15 wk
- Soil No.:
- #2
- % Degr.:
- 43.5
- Parameter:
- CO2 evolution
- Remarks:
- [14CO2]
- Sampling time:
- 15 wk
- Soil No.:
- #3
- % Degr.:
- 32
- Parameter:
- CO2 evolution
- Remarks:
- [14CO2]
- Sampling time:
- 15 wk
- Soil No.:
- #4
- % Degr.:
- 44.5
- Parameter:
- CO2 evolution
- Remarks:
- [14CO2]
- Sampling time:
- 15 wk
- Soil No.:
- #1
- % Degr.:
- 11
- Parameter:
- CO2 evolution
- Remarks:
- [14CO2]
- Sampling time:
- 4 wk
- Soil No.:
- #2
- % Degr.:
- 12.2
- Parameter:
- CO2 evolution
- Remarks:
- [14CO2]
- Sampling time:
- 4 wk
- Soil No.:
- #3
- % Degr.:
- 11.3
- Parameter:
- CO2 evolution
- Remarks:
- [14CO2]
- Sampling time:
- 4 wk
- Soil No.:
- #4
- % Degr.:
- 10
- Parameter:
- CO2 evolution
- Remarks:
- [14CO2]
- Sampling time:
- 4 wk
- Transformation products:
- no
- Evaporation of parent compound:
- no
- Volatile metabolites:
- no
- Residues:
- no
- Details on results:
- 14C-EDTA was (slowly) biodegraded to 14CO2 by all soils (varying in pH and texture) tested from a major agricultural EDTA use area. No detectable quantities of intermediates accumulated under aerobic conditions.
- Conclusions:
- EDTA is (slowly) biodegradable in soils.
Reference
Generally, the variation between duplicates/triplicates was < 8%.
Description of key information
>= 65% degradation in 45 weeks (read across)
Key value for chemical safety assessment
Additional information
Two studies are available investigating the biodegradability of edetic acid in soil. For the determination of biodegradation in soil non-standard investigations under various conditions show that EDTA can be biodegraded in soil under aerobic conditions. The rate of EDTA degradation was determined in different agricultural soils after 4 - 45 weeks of exposure. While degradation rate was 10-12% only after 4 weeks the degradation continued showing 32-45% degradation after 15 weeks and >= 65% after 45 weeks incubation time. This indicate that EDTA is (slowly) biodegradable in soil and thus not persistent in the terrestrial compartment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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