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Diss Factsheets
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EC number: 200-449-4 | CAS number: 60-00-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_environmental-fate-and-pathways.png)
Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
- Principles of method if other than guideline:
- The study was conducted according to the methods described by Branson DR, Blau GE, Alexander HC, and Neely WB (1975). Transactions of the American Fisheries Society, Vol. 104, No. 4: 785-792
- GLP compliance:
- no
- Specific details on test material used for the study:
- RADIOLABELLING INFORMATION
Specific activity 237 uCi/mg, labeled at the acetate group methyl carbon position - Radiolabelling:
- yes
- Vehicle:
- no
- Test organisms (species):
- Lepomis macrochirus
- Details on test organisms:
- Juvenile bluegill, Lepomis macrochirus, having an average wet weight of 0.49 g and a standard length ranging from 3.0 to 5.1 cm, were obtained from a commercial hatchery and maintained under laboratory conditions for a minimum of 2 weeks prior to testing. The fish were held in continuously flowing, carbon-filtered well water having a total hardness of 120 mg/L (as calcium carbonate). The fish were fed frozen brine shrimp twice daily. This was occasionally supplemented with feedings of live Daphnia. A 12-h photoperiod was maintained in the holding facilities.
- Route of exposure:
- aqueous
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 28 d
- Hardness:
- 120 mg/L as CaCo3
- Test temperature:
- 21 °C
- pH:
- 7.4
- Dissolved oxygen:
- near saturation
- Details on test conditions:
- The bioconcentration test system was based on that described by Branson et al..
The test chambers were 37-litre, all-glass aquaria fitted with removable glass covers.
A standpipe was used to maintain the water volume in the aquaria at 30 litres.
The tank effluents were collected in a common drain and filtered through activated carbon prior to their disposal.
A combination of incandescent and fluorescent lighting was controlled by an automatic timer to provide a 12 h photoperiod simulating dusk and dawn with graduating intensities.
The system provided a daylight intensity of approximately 350-550 cd (200-300 footcandles).
A 1 L proportional diluter was modified to deliver two concentrations of the test material.
A peristaltic pump or a multichannel syringe pump was used to meter the stock solutions directly into the diluter mixing chambers.
The entire diluter assembly was contained within a stainless steel housing to minimize the possibility of 14C contamination. All tests were conducted at 21 ± 2°C in carbon-filtered well water.
A flow rate of 10 litres/h, with a 95 percent replacement time of approximately 9 h, was sufficient to maintain the dissolved oxygen levels at greater than 60 percent of saturation. - Nominal and measured concentrations:
- 0.76 and 0.08 mg/L
- Key result
- Type:
- BCF
- Value:
- ca. 1.8 dimensionless
- Basis:
- whole body w.w.
- Time of plateau:
- 28 d
- Calculation basis:
- steady state
- Remarks on result:
- other: Conc. in environment / dose:0.08 mg/L
- Type:
- BCF
- Value:
- ca. 1.1 dimensionless
- Basis:
- whole body w.w.
- Time of plateau:
- 28 d
- Calculation basis:
- steady state
- Remarks on result:
- other: Conc.in environment / dose:0.76 mg/L
- Elimination:
- yes
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Category justification document is attached in chapter 13.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Type:
- BCF
- Value:
- ca. 1.8 dimensionless
- Basis:
- whole body w.w.
- Time of plateau:
- 28 d
- Calculation basis:
- steady state
- Remarks on result:
- other: Conc. in environment / dose:0.08 mg/L
- Key result
- Type:
- BCF
- Value:
- ca. 1.1 dimensionless
- Basis:
- whole body w.w.
- Time of plateau:
- 28 d
- Calculation basis:
- steady state
- Remarks on result:
- other: Conc. in environment / dose:0.76 mg/L
Referenceopen allclose all
The measured concentrations of the test compound averaged approximately 80 percent of the nominal values. There was no substantial difference in measured concentrations during the kinetic (Cwk) and the plateau (Cwp) exposures; the coefficient of variation was less than 10 percent in all cases.
28-Day (Plateau) Method
At exposures of 0.76 and 0.08 mg/L, 14C-EDTA exhibited an extremely low bioconcentration potential.
During continous exposure up to 672 h, the EDTA concentration in fish did not exceed the EDTA concentration in water. The plateau BCF values for EDTA were approximately 1.1 at 0.76 mg/L and 1.8 at 0.08 mg/L, respectively and were independent of the exposure concentration for the range of values tested. After the transfer to clean water, there was an apparent difference between the two exposure levels in the rate of elimination of 14C activity. For fish exposed to 0.76 mg/L, approximately 81 percent of the accumulated 14C residues were eliminated within 336 h, for fish exposed to 0.08 mg/L, only 60 percent of the accumulated 14C residues had been eliminated within a comparable time.
5-Day (Kinetic) Method
The rate constants calculated for 14C-EDTA were independent of the exposure concentrations, although the higher-level exposure may have been clearing somewhat faster. The projected equilibrium BCF values were similar to those observed in the plateau test and, again, serve to emphasize the extremely low bioconcentration potential of EDTA.
Description of key information
Edetic acid does not significantly accumulate in organisms.
Key value for chemical safety assessment
- BCF (aquatic species):
- 1.8 L/kg ww
Additional information
Since no study investigating the bioaccumulation potential is available for edetic acid (CAS No. 60-00-4), the assessment was based on a study conducted with subcategory 1 member Tetrasodium ethylenediaminetetraacetate (CAS No. 64-02-8) in a read across approach. This is in accordance with Regulation (EC) No. 1907/2006, Annex XI, 1.5. Grouping of substance and read across approach. Further justification for suitability of read across between subcategory 1 members is given within the category justification attached to chapter 13.
The key study was conducted according to the methods described by Branson et al. (1975; Transactions of the American Fisheries Society, 104(4): 785-792) and is similar to a flow through OECD 305 test (Bishop and Maki 1980). Acclimated fish were transferred to equilibrated test chambers, which contained nominal exposure concentrations of 1.0 and 0.1 mg/L EDTA. Fish and water samples were periodically collected for 14C analysis. The duration of the uptake phase for the kinetic tests was arbitrarily selected as 120 h (5 days). The uptake phase was continued for 672 h (28 days) for the plateau tests. Samples collected at 48 and 120 h were designated as the initial plateau samples. At exposures of 0.76 and 0.08 mg/L, 14C-EDTA exhibited extremely low 28d-whole fish bioconcentration factors of 1.1 - 1.8. After transfer to clean water, elimination showed 81% at 0.76 mg/l and 60% at 0.08 mg/L in 14 days. In conclusion, the test substance did not bioaccumulate in fish. Based on the suitability of the read across approach this conclusion can also considered to be true for edetic acid (CAS No. 60-00-4).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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