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Diss Factsheets
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EC number: 701-160-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- july 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study (OECD 402)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- yes
- Remarks:
- acclimatation: 4 days
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- GRAPHISTRENGTH C100
- IUPAC Name:
- GRAPHISTRENGTH C100
- Reference substance name:
- Tangled Multi-Walled Carbon Nanotubes
- EC Number:
- 701-160-0
- Cas Number:
- 7782-42-5
- Molecular formula:
- Hollow tubular carbon, 1-dimensional nano structures with hexagonal arrangement of carbon atoms
- IUPAC Name:
- Tangled Multi-Walled Carbon Nanotubes
- Test material form:
- solid: nanoform
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source:Janvier, Le Genest-Saint-Isle, France
- Age at study initiation:approximately 8 weeks old
- Weight at study initiation:287+/- 6g for the males and 214+/- 5g for the females
- Fasting period before study:no
- Housing:During the acclimatation period, 1 to 7 animals of the same sex were housed in polycarbonate cages with stainless steel lid (48cm x 27cm x 20cm). During the treatment period, the animals were housed individually in polycarbonate cages with stainless steel lid (35.5cm x 23.5cm x 19.3cm). Each cage contained autoclaved sawdust, sawdust is analyzed by the supplier for composition and contaminant levels.
- Diet:SsniffR/M-H pelleted diet, ad libitum
- Water:filtered by a FG Millipore membrane (0.22 micron), ad libitum
- Acclimation period:4 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C):22+/-2
- Humidity (%):30 to 70
- Air changes (per hr):approximately 12 cycles/hour of filtered, non-recycled air
- Photoperiod (hrs dark / hrs light):12h/12h
Administration / exposure
- Type of coverage:
- semiocclusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- Purified water was used in order to to moisten the test item and ensure a good contact with a skin.
TEST SITE
- Area of exposure:dorsal area (5cm x 7cm for the males and 5cm x 6cm for the females)
- % coverage:approximately 10%
- Type of wrap if used:a hydrophilic gauze pad (pre-moistened with 2mL of purified water) held in contact with the skin for 24 hours by means of an adhesive hypoallergenic aerated semi-occlusive dressing and a restraining bandage. The dressing prevented from ingestion of the test item by the animal.
REMOVAL OF TEST SUBSTANCE
- Washing (if done):no
- Time after start of exposure:24 hours
- For solids, paste formed: yes - Duration of exposure:
- 24 hours
- Doses:
- 2000 mg/Kg
- No. of animals per sex per dose:
- 5 animals/sex/dose
- Control animals:
- not required
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: frequently during the hours following administration of the test item; thereafter at least once a day until day 15.
-Frequency of weighing:individually, just before administration of the test item on day 1 and then on days 8 and 15.
- Necropsy of survivors performed: yes - Statistics:
- no
Results and discussion
- Preliminary study:
- no
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD0
- Effect level:
- >= 2 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: no mortality
- Mortality:
- no deaths occured during the study
- Clinical signs:
- other: no clinical signs were observed during the study. A black coloration of the skin was noted in all the animals from day 2 until day 10, in all the males and in one female between day 11 and day 15. This coloration masked the evaluation of cutaneous reactio
- Gross pathology:
- macroscopic examination of the main organs of the animals (digestive tract, heart, kidneys, liver, lungs, pancreas, spleen and any other organs with obvious abnormalities) revealed no apparent abnormalities.
- Other findings:
- no
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: other: Regulation n°1272/2008
- Conclusions:
- Under the experimental conditions of this study, the dermal LD0 of the test item GRAPHISTRENGTH C100 micronised was equal to or higher than 2000mg/Kg in rats.
- Executive summary:
The acute dermal toxicity of the test item GRAPHISTRENGTH C100 micronised was evaluated in rats according to OECD 402 and Good Laboratory Practice Regulations. The test item was applied to the skin of one group of ten Sprague-Dawley rats (five males and five females). The application was performed with the test item in its original form at the dose-level of 2000 mg/Kg.
The test site was then covered by a semi-occlusive dressing for 24 hours. Clinical signs, mortality and body weight gain were checked for a period of 14 days following the single application of the test item. All animals were subjected to necropsy.
No deaths and no clinical signs were observed during the study. A black coloration of the skin was noted in all the animals from day 2 until day 10, in all the males and in one female between day 11 and day 15. This coloration masked the evaluation of cutaneous reactionsin all the animals from day 2 until day 6. Crusts were observed in 1/5 males and 2/5 females between day 11 and day 15.
No apparent abnormalities were observed at necropsy in any animal.
The dermal LD0 of the test item GRAPHISTRENGTH C100 micronised was equal to or higher than 2000 mg/Kg in rats.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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