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Diss Factsheets
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EC number: 701-160-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: According to OECD Guideline, GLP compliant
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Each concentration of GRAPHISTRENGTH C100 was prepared as WAF for concentrations varying from 5 mg/L to 100 mg/L (geometric factor close to 2.1) in 2 litres of dilution water.
GRAPHISTRENGTH C100 was maintained in suspension by stirring during distribution in the test flasks. However, after filling and between each renewal, there was a sedimentation of the test item to the bottom of flasks.
Results of the acute toxicity test were used to define the concentration range used in the reproduction test (an EC50 value > 100 mg/L was found on Daphnia magna acute toxicity test)
These preparation was performed for each medium renewal every 2 or 3 days. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Biological reagent: organisms Daphnia magna Straus (Cladocera, Crustacea), clone 5 or clone A. Animals come from a stock breading in the laboratory reared in VOLVIC® water added with vitamin B12, Na2SeO3, 5H2O, spiruline, Calcium, Magnesium unicellular green fresh-water algae Pseudokirchneriella subcapitata and Chlorella vulgaris. Selection of test organisms less than 24 hours old is made by filtration.
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Test temperature:
- 19.4 ± 2.6 °C Variation in temperature was higher than expected, due to a temperature control issue in the laboratory
- pH:
- Fresh solutions: 7.8 - 8.1
- Dissolved oxygen:
- Fresh solutions: 8.9 - 9.3
- Nominal and measured concentrations:
- Nominal concentrations: 0 - 5 - 10 - 22 - 47 - 100 mg/L
- Details on test conditions:
- Dilution water was prepared with Volvic® water complemented as follow:
0.1 ml/L of B12 vitamin solution (1 µg/L)
0.1 ml/L of Na2SeO3.5H2O solution (6.7 µg/L)
1ml/L of calcium / magnesium solution: Ca(NO3)2.4H2O : 208 g/L and MgCl2.6H2O : 28 g/L.
These solutions were prepared with ultra-pure water (resistivity > 18Mohm), reagents « analytical grade ». - Reference substance (positive control):
- yes
- Remarks:
- K2Cr2O7
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 47 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Validity criteria fulfilled:
- yes
- Conclusions:
- Exposure of daphnias to Graphistrength C100 multiwall carbon nanotubes (without added vehicle) resulted in an inhibition of reproductionat 100 mg/L (LOEC). NOEC = 47 mg/L in the conditions of the test.
- Executive summary:
The chronic toxicity (inhibition of reproduction) of the test itemGRAPHISTRENGTH C100toDaphnia magnawas assessed according to the guideline 211 of the OECD.
Daphniawere exposed to a range of concentrations ofGRAPHISTRENGTH C100dispersed in dilution water. The chronic effect measured during the assay was the inhibition of reproduction after time periods of 21 days.
EC50, LOEC and NOEC values are summarised in the following table:
Measured concentrations
EC50Value
LOEC
NOEC
mg/L
317.75
100
47
Reference
GRAPHISTRENGTH C100 Nominal Concentrations (mg/L) |
0.00 |
5.00 |
10.00 |
22.00 |
47.00 |
100.00 |
|
Vessel 1 |
222 | 192 |
213 |
171 |
169 |
26 |
|
Vessel 2 |
193 |
222 |
192 |
190 |
109 |
170 |
|
Vessel 3 |
208 |
180 |
190 |
192 |
169 |
191 |
|
Vessel 4 |
202 |
207 |
201 |
183 |
181 |
158 |
|
Vessel 5 |
218 |
0 |
183 |
189 |
157 |
166 |
|
Vessel 6 |
158 |
174 |
198 |
194 |
170 |
173 |
|
Vessel 7 |
165 |
197 |
185 |
214 |
211 |
156 |
|
Vessel 8 |
183 |
203 |
192 |
156 |
158 |
161 |
|
Vessel 9 |
208 |
186 |
0 |
179 |
176 |
185 |
|
Vessel 10 |
180 |
218 |
184 |
196 |
197 |
143 |
|
Ne Total |
1937 |
1779 |
1738 |
1864 |
1697 |
1529 |
|
Replicates number |
10 |
9 |
9 |
10 |
10 |
10 |
|
Mean |
193.70 |
197.67 |
193.11 |
186.40 |
169.70 |
152.90 |
|
Standard deviation |
21.78 |
64.40 |
61.74 |
15.60 |
27.08 |
46.73 |
|
Coefficient of variation (%) |
11.25 |
36.20 |
35.52 |
8.37 |
15.96 |
30.56 |
|
% Inhibition |
- |
-2.05 |
0.30 |
3.77 |
12.39 |
21.06 |
Description of key information
Inhibition of reproduction of Daphnia magna has been investigated through an OECD 211 study. A NOEC of 47 mg/L (nominal) has been observed.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 47 mg/L
Additional information
An OECD 211 study (inhibition of reproduction of Daphnia magna) gives a NOEC of 47 mg/L (nominal).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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