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Diss Factsheets
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EC number: 264-150-0 | CAS number: 63449-39-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1983
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 475 (Mammalian Bone Marrow Chromosome Aberration Test)
- GLP compliance:
- yes
- Type of assay:
- chromosome aberration assay
Test material
- Reference substance name:
- Paraffin waxes and Hydrocarbon waxes, chloro
- EC Number:
- 264-150-0
- EC Name:
- Paraffin waxes and Hydrocarbon waxes, chloro
- Cas Number:
- 63449-39-8
- Molecular formula:
- C18H33Cl5 C18H30Cl8 C20H36Cl6 C20H33Cl9 C25H45Cl7 C25H42Cl10 C25H29C23 C30H53Cl9 C30H49Cl13 C30H35Cl27
- IUPAC Name:
- Paraffin waxes and Hydrocarbon waxes (C18 and longer), chloro
- Details on test material:
- C20-30; 70% Cl (solid)
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Fischer 344
- Sex:
- male
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- vehicle of 1% carboxymethylcellulose in water
- Details on exposure:
- The CP treatment and 1% carboxymethylcellulose control animals were all dosed once daily by oral gavage for 5 days. The cyclophosphamide and saline control groups were dosed via i.p. 36 and 12 hours before scheduled sacrafice. Approximately 6 hours prior to sacrafice all animals were administered colchicine, 5 mg/kg, i.p. to arrest mitosis at C-metaphase.
- Duration of treatment / exposure:
- 5 days
- Frequency of treatment:
- daily
Doses / concentrations
- Remarks:
- Doses / Concentrations:
500, 1500, 5000 mg/kg /day
Basis:
actual ingested
- No. of animals per sex per dose:
- 8
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- Cyclophosphamide (40 mg/kg), the positive control, was administered by i.p. injection to groups of 8 male Fischer 344 rats on days 5 and 6 of the study, 36 and 12 hours prior to scheduled termination, respectively . Control groups of 8 rats received i.p. injections of saline on the same schedule.
Examinations
- Tissues and cell types examined:
- Bone marrow
- Details of tissue and slide preparation:
- Femurs were dissected, bone marrow collected and slides of metaphase spreads were prepared. Up to 100 smears were examined from each rat receiving the test compound (less for positive controls).
- Statistics:
- Statistical evaluation of mutation frequencies was made by the method described by Kastenbaum and Bowman.
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
No deaths occurred during the exposure period. Body weight gain was reduced in rats receiving 5000 mg/kg test material, compared to controls. No treatment related signs of toxicity were noted. No increases in the frequency of chromosomal abnormalities were observed in the treated groups when compared to the controls. The positive agent, cyclophosphamide, produced a highly statistically significant frequency of chromosomal aberrations per cell, with nearly 70% of all spreads examined appearing abnormal. The high frequency of chromosomal aberrations noted for the positive control group indicates the experimental system to be appropriate and sensitive. No specific evidence was presented to demonstrate that the test article reached the target organ at doses up to 5000 mg/kg po, the limit dose applied in the study design.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
No increases in the frequency of chromosomal abnormalities were observed in the treated groups when compared to the controls. The positive agent, cyclophosphamide, produced a highly statistically significant frequency of chromosomal aberrations per cell, with nearly 70% of all spreads examined appearing abnormal. The high frequency of chromosomal aberrations noted for the positive control group indicates the experimental system to be appropriate and sensitive. No specific evidence was presented to demonstrate that the test article reached the target organ at doses up to 5000 mg/kg po, the limit dose applied in the study design. - Executive summary:
No increases in the frequency of chromosomal abnormalities were observed in the treated groups when compared to the controls.
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