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Diss Factsheets
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EC number: 203-149-1 | CAS number: 103-83-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Remarks:
- German standard DIN 38412, part 8
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: German standard DIN 38412, part 8
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- Strain: DSM 50026
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 17 h
- Nominal and measured concentrations:
- Nominal test concentrations (mg/l): 15.625, 31.25, 62.5, 125, 250, 500, 1000, control
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer vessel (300 ml)
- Fill volume: 100 ml
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: Bacterial suspension 10 TE/F
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: AK-Medium DIN 38412 part 8 draft - Duration:
- 17 h
- Dose descriptor:
- EC10
- Effect conc.:
- 534 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 17 h
- Dose descriptor:
- EC50
- Effect conc.:
- 749.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 17 h
- Dose descriptor:
- other: EC90
- Effect conc.:
- 965.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Conclusions:
- Benzyldimethylamine revealed a EC10 of 534 mg/L and a EC50 of 749.6 mg/L in a 17 h growth inhibition test with Pseudomonas putida.
- Executive summary:
A growth inhibition test according to Bringmann and Kuehn (German statndad DIN 38412, part 8) was conducted to assess the toxicity of Benzyldimethylamine to bacteria (Pseudomonas putida, strain DSM 50026). Nominal test concentrations were 0, 15.625, 31.25, 62.5, 125, 250, 500, 1000 mg/L. The growth inhibition was determined after a period of 17 hours. Benzyldimethylamine revealed a EC10 of 534 mg/L and a EC50 of 749.6 mg/L.
Reference
pH values:
Test conc.(mg/l) |
pH values after |
|
0 hours, uninoculated |
17 hours, inoculated |
|
15.625 |
7.54 |
8.59 |
31.25 |
7.62 |
8.58 |
62.5 |
7.78 |
8.59 |
125 |
8.11 |
8.55 |
250 |
9.01 |
8.46 |
500 |
9.38 |
8.39 |
1000 |
9.62 |
7.94 |
blank |
7.54 |
8.61 |
Results:
Test item [mg/L] |
% of growth in blank |
15.625 |
97.04 |
31.25 |
96.77 |
62.5 |
98.86 |
125 |
99.32 |
250 |
100.14 |
500 |
96.3 |
1000 |
3.56 |
Description of key information
The effects of benzyldimethylamine to the population growth of Pseusomonas putida was determined according to Bringmann and Kühn (DIN 38412, part 8, 17 h). Benzyldimethylamine revealed an EC10 of 534 mg/L and an EC50 of 749.6 mg/L (BASF, 1987).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 749.6 mg/L
- EC10 or NOEC for microorganisms:
- 534 mg/L
Additional information
Key study:
A growth inhibition test according to Bringmann and Kuehn (German statndad DIN 38412, part 8) was conducted to assess the toxicity of Benzyldimethylamine to bacteria (Pseudomonas putida, strain DSM 50026). Nominal test concentrations were 0, 15.625, 31.25, 62.5, 125, 250, 500, 1000 mg/L. The growth inhibition was determined after a period of 17 hours. Benzyldimethylamine revealed an EC10 of 534 mg/L and an EC50 of 749.6 mg/L (BASF, 1987).
Supporting/disregarded studies:
- A respiration inhibition test according OECD 209 (activated sludge) was conducted to assess the toxicity of Benzyldimethylamine to bacteria. Nominal test concentrations were 0, 115, 269 and 575 mg/L. The growth inhibition was determined after a period of 30 min at a temperature of 20-25 °C. Benzyldimethylamine revealed an EC20 of 575 mg/L (BASF, 1987).
- A growth inhibition test according to Bringmann and Kuehn was conducted to assess the toxicity of Benzyldimethylamine to bacteria (Pseudomonas putida) (Bayer AG, 1979). Nominal test concentrations were 0, 1.96, 3.91, 7.82, 15.63, 31.25, 62.5, 125, 250, 500, 1000 mg/L. The growth inhibition was determined after a period of 16 hours. Benzyldimethylamine revealed an EC10 of 54 mg/L. This, non state of the art study was not taken into account in the risk assessment .
Conclusion:
The risk assessment for STP will be based on the growth inhibition test with Pseudomonas putida revealing an EC10 of 534 mg/L (BASF 1987). This value is supported by a respiration inhibition test (activated sludge according to OECD 209) with an EC20 of 575 mg/L (BASF, 1987). The study by Bayer AG revealed an EC10 of 54 mg/L and is thus contradictionary to both BASF tests. This non guideline and non GLP study is thus not taken into account for the risk assessment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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