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EC number: 200-843-6 | CAS number: 75-15-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: According to: OECD Guideline for Testing of Chemicals 437: Bovine Corneal Opacity and Permeability Test Method for Identifying Ocular Corrosives and Severe Irritants (September, 2009).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline for Testing of Chemicals 437: Bovine Corneal Opacity and Permeability Test Method for Identifying Ocular Corrosives and Severe Irritants (September, 2009).
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- carbon disulfide
- IUPAC Name:
- carbon disulfide
- Details on test material:
- Identity: Carbon disulfide (provided by AkzoNobel)
Batch-No.: 0600809041160
CAS-No.: 75-15-0
EC-No.: 200-843-6
Purity: > 99%
Stability in Solvent: Not relevant
Storage: At room temperature, under Nitrogen, avoid vapour release and contact of vapour with open flame and/or hot surfaces
Expiration Date: September 19, 2011
Constituent 1
Test system
- Amount / concentration applied:
- 0.75 mL of the undiluted liquid test item were applied to the isolated corneas
- Duration of treatment / exposure:
- The corneas were exposed to the test item for 10 minutes
- Details on study design:
- Corneas were treated with the undiluted test item for 10 minutes. After treatment the test item was washed off the corneas and they were further incubated for 2 hours. The opacity of the corneas was determined after the two hours incubation. The change in permeability of the corneas was determined after 90 minutes treatment with fluorescein solution.
Results and discussion
In vitro
Results
- Irritation parameter:
- cornea opacity score
- Value:
- 0.37
- Remarks on result:
- other: see below for details
- Other effects / acceptance of results:
- The test item Carbon disulfide did not cause any opacity or permeability of the corneae compared with the results of the negative control. The calculated mean in vitro score was 0.37 and therefore, the test item was classified as non eye irritant.
Any other information on results incl. tables
Results after 10 Minutes Incubation Time
Test Group |
Opacity value = Difference (t130-t0) of Opacity |
Permeability at 490 nm (OD490)* |
In vitro score |
Mean in vitro score |
Proposed in vitro irritation Scale |
||
|
|
Mean |
|
Mean |
|
|
|
Negative Control |
- 1 |
- 0.33 |
0.049 |
0.047 |
- 0.27 |
0.38 |
Non eye irritant |
0 |
0.047 |
0.71 |
|||||
0 |
0.046 |
0.69 |
|||||
Positive Control |
58.33* |
0.856* |
71.17 |
77.34 |
Severe eye irritant |
||
66.33* |
0.885* |
79.60 |
|||||
69.33* |
0.794* |
81.24 |
|||||
Carbon disulfide |
0.33* |
0.008* |
0.45 |
0.37 |
Non eye irritant |
||
0.33* |
0.001* |
0.34 |
|||||
0.33* |
- 0.001* |
0.31 |
*corrected values
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- In conclusion, it can be stated that in this study and under the experimental conditions reported, the test item Carbon disulfide is not considered to be an eye irritant.
- Executive summary:
This in vitro study was performed to assess the corneal irritation and damage potential of carbon disulfide by means of the BCOP assay using fresh bovine corneas. After a first opacity measurement of the fresh bovine corneae (t0), carbon disulfide, the positive, and the negative controls were applied to corneas and incubated for 10 minutes at 32±2 °C in complete medium. After the incubation phase the test item, the positive, and the negative controls were each rinsed off the corneas and opacity was measured again (t10). Further, the corneas were incubated for another 120 minutes at 32±2 °C in complete medium, and opacity was measured a third time (t130). After the opacity measurements permeability of the corneas was determined while application of 1 mL of a fluorescein solution for 90 minutes at 32±2 °C in a horizontal position. The liquid coming out was measured spectrophotometrically.
With the negative control (0.9% NaCl solution) neither an increase of opacity nor permeability of the corneae could be observed. The mean in vitro score was calculated as 0.38. The positive control (2-Ethoxyethanol) showed clear opacity and distinctive permeability of the corneae and therefore, is classified as severe eye irritant. The mean in vitro score was calculated as 77.34. The test item carbon disulfide did not cause any opacity or permeability of the corneae compared with the results of the negative control. The calculated mean in vitro score was 0.37 and therefore, the test item was classified as non eye irritant.
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