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EC number: 200-843-6 | CAS number: 75-15-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: According to: OECD Guideline for the Testing of Chemicals, Draft Proposal for a New Guideline, In Vitro Skin Irritation: Reconstructed Human Epidermis (RhE) Test Method, 11 December 2009, Vers. 4.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline for the Testing of Chemicals, Draft Proposal for a New Guideline, In Vitro Skin Irritation: Reconstructed Human Epidermis (RhE) Test Method, 11 December 2009, Vers. 4.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Carbon disulphide
- EC Number:
- 200-843-6
- EC Name:
- Carbon disulphide
- Cas Number:
- 75-15-0
- Molecular formula:
- CS2
- IUPAC Name:
- methanedithione
- Details on test material:
- Identification: Carbon disulfide
Source: Supplied by Akzo Nobel
EC Number: 200-843-6
CAS Number: 75-15-0
Batch Number: 0600809041160
Molecular Weight: 76.1 g/mol
Purity: > 99 % (dose calculation not adjusted to purity) (Certificate of analysis provided by the supplier)
Stability in Solvent: Not indicated by the sponsor
Expiry Date: September 19, 2011
Storage Conditions: At room temperature, under Nitrogen, avoid vapour release and contact of vapour with open flame and/or hot surfaces.
Storage Conditions at Harlan Laboratories: At room temperature at about 20 °C, N2
Constituent 1
Test system
- Type of coverage:
- other: in vitro testing
- Amount / concentration applied:
- Application volume: 15 µL per skin tissue
Test concentration: undiluted - Duration of treatment / exposure:
- 15 minutes treatment followed by 42 hours further incubation without test item
- Details on study design:
- One experiment was performed. Three skin tissues (EpiSkin TM Tissues) were treated with each 15 µL of the undiluted test item. The tissues were exposed to the test item for 15 minutes. Afterwards the test item was washed off the tissues and they were incubated for further 42 hours. The evaluation was done via MTT conversion.
Negative control: deionised water (15 µL applied to each of the triplicate of tissues)
Positive control: 5% SLS (Sodium lauryl sulphate) solution in deionised water (15 µL applied to each of the triplicate of tissues)
Results and discussion
In vitro
Results
- Irritation / corrosion parameter:
- other: relative absorbance value (%)
- Value:
- 67
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other:
- Remarks:
- all 3 tissues. Time point: 15 min. Remarks: see below for details
- Other effects / acceptance of results:
- After treatment with carbon disulfide the relative absorbance values were decreased to 67.0%. This value is well above the threshold for irritancy of 50%. Therefore, the test item is not considered to possess an irritant potential.
Any other information on results incl. tables
Table 1: Results after treatment with carbon disulfide
Dose group |
Treatment Interval |
Absorbance 570 nm |
Absorbance 570 nm |
Absorbance 570 nm |
Mean Absorbance of 3 Tissues |
Standard Deviation |
Rel. Absorbance [% of Negative Control]** |
Negative Control |
15 min |
0.667 |
0.687 |
0.662 |
0.672 |
2.0 |
100.0 |
Positive Control |
15 min |
0.341 |
0.176 |
0.294 |
0.270 |
12.6 |
40.2 |
Carbon disulfide |
15 min |
0.460 |
0.498 |
0.393 |
0.450 |
7.9 |
67.0 |
* Mean
of three replicate wells after blank correction
** relative
absorbance [rounded values]
Optical evaluation of the MTT-reducing capacity of the test item after 1 hour incubation with MTT-reagent did not show blue colour.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Remarks:
- Migrated information Criteria used for interpretation of results: OECD GHS
- Conclusions:
- In conclusion, it can be stated that in this study and under the experimental conditions reported, the test item carbon disulfide is non irritant to skin.
- Executive summary:
This in vitro study was performed to assess the irritation potential of Carbon disulfide by means of the Human Skin Model Test. Three tissues of the human skin model EpiSkin™ were treated with the test item, the negative or the positive control for 15 minutes. 15 µL of the test item were applied to each tissue, spread to match the tissue size. The test item, the positive and negative controls were washed off the skin tissues after 15 minutes treatment. After further incubation for about 42 hours the tissues were treated with the MTT solution for 3 hours following approximately 70 hours extraction of the colorant from the cells. The amount of extracted colorant wasdetermined photometrically at 570 nm. 15 µL of either the negative control (deionised water) or the positive control (5% Sodium lauryl sulfate) were applied to each tissue. After treatment with the negative control the absorbance values were well above the required acceptability criterion of mean 0.6≤OD≤1.5 for the15 minutestreatment interval thus showing the quality of the tissues. Treatment with the positive control induced a decrease in the relative absorbance as compared to the negative control to 40.2%. The standard deviations between the % variabilities of the test item, the positive and negative controls were below 8% (threshold of the "OECD Guideline for the Testing of Chemicals, Draft Proposal for a New Guideline,In Vitro Skin Irritation": 18%), thus ensuring the validity of the study. After treatment with the test item Carbon disulfide the relative absorbance values were decreased to 67.0%. This value is well above the threshold for irritancy of 50%. Therefore, the test item is not considered to possess an irritant potential.
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