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Diss Factsheets
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EC number: 800-940-9 | CAS number: 35836-72-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 February – 20 May 1977
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- Study similar to OECD Guideline No 401 with deviations: no data test substance purity, no certificate of analysis ; no. of animals at two dose levels < 5; no details on environmental conditions; observation period of 7 days instead of 14 days.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 977
- Report date:
- 1977
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- yes
- Remarks:
- (no data about test substance purity and no certificate of analysis; no. of animals at two dose levels < 5; no details on environmental conditions; observation period: 7 days)
- Principles of method if other than guideline:
- not applicable
- GLP compliance:
- no
- Remarks:
- pre-dating GLP regulation
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- (1R,5S)-2-(6,6-dimethylbicyclo[3.1.1]hept-2-en-2-yl) ethyl acetate
- EC Number:
- 800-940-9
- Cas Number:
- 35836-72-7
- Molecular formula:
- C13H20O2
- IUPAC Name:
- (1R,5S)-2-(6,6-dimethylbicyclo[3.1.1]hept-2-en-2-yl) ethyl acetate
- Test material form:
- liquid
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- not specified
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
Age at study initiation: 4-5 weeks
Housing: animals were housed in individual cages.
Fasting period before study: 4 h
Diet: commercial pelleted diet, ad libitum
Water: ad libitum
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
- MAXIMUM DOSE VOLUME ADMINISTERED: 10 mL/kg bw
- Doses:
- 2, 5 and 10 mL/kg bw (equivalent to 1960, 4900 and 9800 mg/kg bw)
- No. of animals per sex per dose:
- 5 mL/kg bw: 3/sex/dose
2 and 10 mL/kg bw: 1/sex/dose - Control animals:
- no
- Details on study design:
- Duration of observation period following administration: 7 days
Any animal dying during the test was autopsied.
Survivors were weighed before killing for post-mortem examination at the end of the one week observation period. - Statistics:
- none
Results and discussion
- Preliminary study:
- not applicable
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 2 - 5 mL/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: equivalent to 1960-4900 mg/kg bw; specific gravity: 0.98
- Mortality:
- Mortalities at 2, 5 and 10 mL/kg were 0, 100 and 100 %, respectively.
- Clinical signs:
- other: Mice dosed at all three levels were hypothermic and showing signs of stress within 30 minutes after treatment. Females dosed 5 and 10 mL/kg bw were also somnolent and exhibiting laboured breathing. Within 1 h after treatment all the male mice were somno
- Gross pathology:
- Autopsy of the animals that died revealed irritation of the small intestine and peritoneum; and the mesenteric lymph nodes of these animals also appeared enlarged and inflammed.
Livers and kidneys of these animals were pale, and they had distended bladders. - Other findings:
- none
Any other information on results incl. tables
none
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The oral LD50 of nopyl acetate could be considered as higher than 2000 mg/kg bw in mice therefore it is not classified for acute oral toxicity according to Directive 67/548/EEC and CLP Regulation (EC) No 1272/2008.
- Executive summary:
In an acute oral toxicity study (similar to OECD Guideline No 401), 3 groups of 1, 3 and 1 mice/sex were given a single oral dose of nopyl acetate at 2, 5 and 10 mL/kg bw, respectively. Animals were then observed for mortality, clinical signs and bodyweights for 7 days and were all macroscopically necropsied after sacrifice.
Mortalities at 2, 5 and 10 mL/kg bw were 0, 100 and 100 %, respectively. Surviving animals gained weight during the 7 day observation period and presented a normal appearance at autopsy. Mice dosed at all three levels were hypothermic and showing signs of stress within 30 minutes after treatment. Female dosed 5 and 10 mL/kg bw were also somnolent and exhibiting laboured breathing. Within 1 hr after treatment all the male mice were somnolent and the females dosed at 5 and 10 mL/kg bw were comatose. Within 2 hr after treatment all mice dosed at 5 and 10 mL/kg bw were cyanosed and after 18 h all these animals were dead. Mice dosed at 2 mL/kg bw were still showing signs of stress 18 h after treatment, but recovered within 42 h. Autopsy of the animals that died revealed irritation of the small intestine and peritoneum; and the mesenteric lymph nodes of these animals also appeared enlarged and inflammed. Livers and kidneys of these animals were pale, and they had distended bladders. In this study, the combined oral LD50 of nopyl acetate was considered to be 2-5 mL/kg bw (equivalent to 1960-4900 mg/kg bw) in mice.
The oral LD50 of nopyl acetate could be considered as higher than 2000 mg/kg bw in mice therefore it is not classified for acute oral toxicity according to Directive 67/548/EEC and CLP Regulation (EC) No 1272/2008.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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