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EC number: 433-460-1 | CAS number: 210880-92-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Sep 1996 - Oct 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Version / remarks:
- adopted 1987
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- -
- EC Number:
- 433-460-1
- EC Name:
- -
- Cas Number:
- 210880-92-5
- Molecular formula:
- C6H8ClN5O2S
- IUPAC Name:
- (E)-N'-[(2-chloro-1,3-thiazol-5-yl)methyl]-N-methyl-N''-nitroguanidine
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- CD-1
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River (UK) Ltd, Margate, UK
- Females nulliparous and non-pregnant: yes
- Age at study initiation: 5 - 7 weeks
- Weight at study initiation: 25 to 30 g (males), 22 to 26 g (females)
- Fasting period before study: from approximately 4 h prior to dosing until 2 h after completion of dosing
- Housing: up to five mice of the same sex were accommodated in suspended stainless steel mesh cages (dimensions 32 x 16.5 x 13 cm)
- Diet: SQC(E) Rat and Mouse Maintenance Diet No 1 (Special Diets Services Ltd, Witham, UK), ad libitum
- Water: mains water, ad libitum
- Acclimation period: 6 - 9 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19 - 25
- Humidity (%): 40 - 70
- Air changes (per hr): at least 15
- Photoperiod (hrs dark / hrs light): 12/12
IN-LIFE DATES: From: 16 Sep 1996 To: 03 Oct 1996 (main study)
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: 5% m/v aqueaous gum arabic
- Details on oral exposure:
- VEHICLE
- Amount of vehicle: 10 mL/kg bw - Doses:
- 304, 380, 475, 594 and 742 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: regularly during the study at least once within half an hour of dosing, four times within four hours of dosing, twice daily on Days 2, 3 and 4 and once daily from the fifth to last day of the observation period; body weights were recorded on Day -1 (day before dosing), Day 1 (day of dosing), Days 4 and 8 and at termination on Day 15.
- Necropsy of survivors performed: yes - Statistics:
- Not reported.
Results and discussion
- Preliminary study:
- A preliminary investigation was conducted using groups of two male and two female fasted mice. Mice were subjected to single oral doses of 300, 450 and 600 mg/kg bw. Two male mice
and one female were found dead on Day 2 following treatment at 600 mg/kg bw. There were no deaths among the mice dosed at 300 or 450 mg/kg bw. Based on the results, suitable dose levels to begin determination of the acute median lethal dose were selected for the main study.
Effect levelsopen allclose all
- Key result
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 389 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 348 - 433
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 465 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 384 - 561
- Mortality:
- 304 mg/kg bw: 0/5 (males); 0/5 (females)
380 mg/kg bw: 2/5 (males); 2/5 (females)
475 mg/kg bw: 5/5 (males); 3/5 (females)
594 mg/kg bw: 5/5 (males); 3/5 (females)
742 mg/kg bw: 5/5 (males); 5/5 (females)
For details, please refer to the attached background material. - Clinical signs:
- other: The principal clinical signs of reaction to treatment at all dose levels were decreased activity and palpebral closure starting 0.25 h after treatment. Less common clinical signs included ataxia and tremor at all dose levels starting 0.25 h after treatmen
- Gross pathology:
- Necropsy of mice found dead, as well as those killed at completion of the 14-day observation period revealed no macroscopic changes consistent with the treatment-related effect. Isolated cases of darkening of the lungs, fluid accumulation in the thoracic cavity, distension of the uterus or cannibalisation of the genitalia were noted. For details, please refer to the attached background material.
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- The study was performed in accordance with OECD TG 401 under GLP conditions and is considered reliable. Under the conditions chosen, the acute oral LD50 value was determined to be 389 mg/kg bw and 465 mg/kg bw for male and female mice, respectively. According to criteria of the CLP Regulation (EU) No. 1272/2008, classification of the test item for acute oral toxicity category 4 is needed.
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