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EC number: 438-930-8 | CAS number: 2550-52-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 - 29 Mar 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- Apr 1984
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- Dec 1992
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Niedersächsisches Umweltministerium, Hannover, Germany
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control, limit concentration 100 mg/L (nominal concentration)
- Sample storage conditions before analysis: if necessary, samples were stored at 7 ± 2 °C until analysis - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: a stock solution of 100 mg/L (nominal concentration) was prepared in dilution water, which was subject to a 30 min dispersion treatment (ultraturrax at 8500 rpm) and then filtered with a 45 µm single-use filter after dispersion
- Controls: yes, dilution water
- Evidence of undissolved material: no, the test item was clearly dissolved throughout exposure. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: Clone 5
ACCLIMATION
- Acclimation period: 2 h in dilution water
- Acclimation conditions (same as test or not): same
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
2 - 24 h old daphnids were used for the definitive study. They were obtained by removing the mother animals twice within 22 h. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 261 mg/L CaCO3
- Test temperature:
- 20 ± 2 °C
- pH:
- 7.61 - 7.75
- Dissolved oxygen:
- 7.81 - 8.59 mg/L
- Nominal and measured concentrations:
- nominal: control, and 100 mg/L
measured: control, and 0.19 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 mL glass beakers
- size, headspace, fill volume: Size: 50 mL, inner diameter = 5 cm, height = 8 cm; Headspace: 30 mL; Fill volume: 20 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to EEC 92/69 L383A C.2.
- Culture medium different from test medium: yes, culture medium = Elendt M4 (1990), modified to a total hardness of 160 to 180 mg/L CaCO3, test medium = dilution water
- Intervals of water quality measurement: the water parameters of the dilution (pH, dissolved O2, temperature, conductivity, total hardness) was measured before test start. The water parameters of the test (pH, dissolved O2) were measured at the beginning of the test in one replicate of test and control and after 48 h in all replicates per concentration and control. Temperature was measured continuously.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light/8 h dark cycle
- Light intensity: diffuse light, 1.5 - 5 µmol * m-2 * s
EFFECT PARAMETERS MEASURED:
- % immobility: after 24 h and 48 h
RANGE-FINDING STUDY
- Test concentrations: First preliminary test: 1:1, 1:10; 1:100 dilutions of the stock solution of 100 mg/L; Second preliminary test: 100 mg/L stock solution with two different dispersion treatments (10 min ultrasonic treatment at room temperature or 30 min ultraturrax at 8500 rpm)
- Results used to determine the conditions for the definitive study: yes, based on the results of the preliminary tests a limit test with a saturated stock solution was performed. Two preliminary range finding tests were conducted under static conditions. In the first preliminary test three dilutions of a saturated stock solution (1:1, 1:10; 1:100, NON-GLP-state) were prepared. For the stock solution 100 mg/L was weighed out. The stock solution was stirred 24 h and filtered with 45 µm filters before use. The second preliminary test was conducted with two different dispersion treatments of the stock solution 100 mg/L: 10 min. ultrasonic treatment at room temperature and 30 min treatment with an ultraturrax at 8500 rpm. Both solutions were filtered with 45 µm filters before use. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.19 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: no
- Mortality of control: 0%
- Any observations that might cause a difference between measured and nominal values: no, the test item was clearly dissolved throughout exposure - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 (24 h) = 2.1 mg/L potassium dichromate - Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 (48 h) > 0.19 mg/L (mean measured saturated concentration, limit test at a nominal concentration of 100 mg/L, OECD 202, Daphnia magna)
Reference
The effect concentration was expressed as the mean measured saturated limit concentration.
Description of key information
EC50 (48 h) > 0.19 mg/L (mean measured saturated concentration, limit test at a nominal concentration of 100 mg/L, OECD 202, Daphnia magna)
Key value for chemical safety assessment
Additional information
There is one GLP certified guideline study available, which assessed the acute immobilisation of the test substance to aquatic invertebrates according to the OECD guideline 202.
In a static test Daphnia magna was exposed to the nominal limit concentration of 100 mg test substance/L for 48 h. The limit concentration was based on a saturated stock solution.
The test substance was analytically verified by GC-FID at the limit concentration and control at 0 h (fresh media) and 48 h (old media). The measured concentration of the test item was 0.19 mg/L at 0 h and 0.18 mg/L at 48 h. Test concentrations are based on the measured initial concentration of the test solution.
No immobilization was observed at the tested limit concentration and the 48 h EC50 was therefore > 0.19 mg/L (measured).
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