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EC number: 231-617-5 | CAS number: 7652-64-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
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- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Version / remarks:
- 1981
- Deviations:
- no
- GLP compliance:
- no
- Remarks:
- This study was conducted prior to creation of the REACH regulation. The study was well documented and is sufficient for classification.
Test material
- Reference substance name:
- 1,1'-(1,3-phenylenedicarbonyl)bis[2-methylaziridine]
- EC Number:
- 231-617-5
- EC Name:
- 1,1'-(1,3-phenylenedicarbonyl)bis[2-methylaziridine]
- Cas Number:
- 7652-64-4
- Molecular formula:
- C14H16N2O2
- IUPAC Name:
- 2-methyl-1-[3-(2-methylaziridine-1-carbonyl)benzoyl]aziridine
Constituent 1
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: The test material was stored frozen.
FORM AS APPLIED IN THE TEST (if different from that of starting material): The test material was warmed to approximately 28°C and administrated undiluted.
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: HRP, Inc.
- Weight at study initiation: 3003 g
- Housing: The animal was individually housed in a screen-bottom cage in temperature- and humidity-controlledquarter
- Diet (e.g. ad libitum): ad libitum; Laboratory Rabbit Diet HF #5326, PMI Feeds, Inc.
- Water (e.g. ad libitum): ad libitum
- Acclimation period: At least 7 days
IN-LIFE DATES: From: 18 March 1994 To: 15 April 1994
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 mL - Duration of treatment / exposure:
- Single exposure; the eye remained unwashed
- Observation period (in vivo):
- 1, 24, 48, 72, and 96 hours and days 7, 14, 21, and 28 after treatment
- Number of animals or in vitro replicates:
- 1 female
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): The eye remained unwashed
- Time after start of exposure: N/A
SCORING SYSTEM: Draize method (1959)
(1) Cornea
(A) Opacity - Degree of density (area most dense taken for reading)
No opacity ................................................................................ 0
Scattered or diffuse area, details of iris clearly visible................................1'
Easily discernible translucent areas, details of iris slightly obscured...................2*
Opalescent areas, no details of iris visible, size of pupil barely discernible............3*
Opaque. iris invisible..................................................................4.*.
(B) Area of Cornea Involved
One-quarter (or less), but not zero......................................................1.
Greater than one-quarter, but less than half.............................................2.
Greater than half. but less than three-quarters..........................................3.
Greater than three-quarters up to whole area.............................................4.
A x B x 5 Total Maximum = 80
(2) Iris
(A) Values
Normal .................................................................................... 0
Folds above normal, congestion, swelling, circumcorneal injection (any or all of these or combination of any thereof) iris is still reacting to light (sluggish reaction is positive)……………..1*
No reaction to light, hemorrhage, gross destruction (any or all of these).................2*
A x 5 Total Maximum= 10
(3) Conjunctivae
(A) Redness (refers to palpebral and bulbar conjunctivae excluding cornea and iris)
Vessels normal..........................................................................0..
Vessels definitely injected above normal .................................................. 1
More diffuse, deeper crimson red, individual vessels not easily discernible...............2*
Diffuse beefy red ..................................................... .................... 3*
(B) Chemosis
No swelling ............................................................................... O
Any swelling above normal (includes nictitating membrane).................................1
Obvious swelling with partial eversion of the lids ........................................ 2*
Swelling with lids about half closed....................................................3.*.
Swelling with lids about half closed to completely closed................................. 4*
(C) Discharge
No discharge...........................................................................0
Any amount different from normal (does not include small amounts observed in inner canthus of normal animals) ................................................................ 1
Discharge with moistening of the lids and hairs just adjacent to the lids.................2
Discharge with moistening of the lids and hairs, and considerable area around the eye.....3
Score (A + B + C) x 2 Total Maximum = 20
The total score for the eye is the sum of all scores obtained for the cornea, iris, and conjunctivae.
* Indicates a positive effect. (FHSA Interpretation)
TOOL USED TO ASSESS SCORE: Sodium fluorescein examinations were used to aid in revealing possible corneal injury at 24 and 72 hours and days 7, 14, 21, and 28.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- overall irritation score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 43
- Reversibility:
- not fully reversible within: 28 days
- Irritation parameter:
- overall irritation score
- Basis:
- animal #1
- Time point:
- other: 96 hour
- Score:
- 41
- Reversibility:
- not fully reversible within: 28 days
- Irritation parameter:
- overall irritation score
- Basis:
- animal #1
- Time point:
- 7 d
- Score:
- 49
- Reversibility:
- not fully reversible within: 28 days
- Irritation parameter:
- overall irritation score
- Basis:
- animal #1
- Time point:
- 14 d
- Score:
- 34
- Reversibility:
- not fully reversible within: 28 days
- Irritation parameter:
- overall irritation score
- Basis:
- animal #1
- Time point:
- 21 d
- Score:
- 34
- Reversibility:
- not fully reversible within: 28 days
- Irritation parameter:
- overall irritation score
- Basis:
- animal #1
- Time point:
- other: 28 days
- Score:
- 32
- Reversibility:
- not fully reversible within: 28 days
- Irritant / corrosive response data:
- The test article produced corneal and iridal involvement and severe conjunctival irritation. Ocular irritation was still present in the rabbit at day 28 after treatment. The sodium fluorescein examination at 24 and 72 hours was positive and at days 7, 14, 21 and 28 was negative. A maximum average primary eye irritation score of 49 of a possible 110.0 was assigned at the 7 day evaluation.
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (irreversible effects on the eye) based on GHS criteria
- Conclusions:
- Based on the results of the study, the test article is corrosive to the eyes.
- Executive summary:
The objective of this study was to assess the relative level of irritation/corrosion produced following a single exposure of a test material to one eye of an albino rabbit. The study was conducted according to OECD 405 (1981). One albino rabbit (New Zealand White strain) received 0.1 mL of the test article in the conjunctival sac of one eye. The contralateral eye served as an untreated control. The eye was evaluated for irritation at 1, 24, 48, 72 and 96 hours and at 7, 14, 21 and 28 days after treatment. At the 24 and 72 hour and 7, 14, 21 and 28 day evaluations, sodium fluorescein was used to aid in revealing possible corneal injury. The test article produced corneal and iridal involvement and severe conjunctival irritation. Ocular irritation was still present in the rabbit at day 28 after treatment. The sodium fluorescein examination at 24 and 72 hours was positive and at days 7, 14, 21 and 28 was negative. A maximum average primary eye irritation score of 49 of a possible 110.0 was assigned at the 7 day evaluation. Based on the results of the study, the test article is corrosive to the eyes.
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