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Diss Factsheets
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EC number: 231-617-5 | CAS number: 7652-64-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 6 Mar - 5 Apr 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1,1'-(1,3-phenylenedicarbonyl)bis[2-methylaziridine]
- EC Number:
- 231-617-5
- EC Name:
- 1,1'-(1,3-phenylenedicarbonyl)bis[2-methylaziridine]
- Cas Number:
- 7652-64-4
- Molecular formula:
- C14H16N2O2
- IUPAC Name:
- 2-methyl-1-[3-(2-methylaziridine-1-carbonyl)benzoyl]aziridine
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Physical state: viscous colorless liquid at room temperature (22-23 °C)
- Source and lot/batch No.of test material: Lot 10115
- Expiration date of the lot/batch: 10 Jan 2019
- Purity: 95.6%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: frozen
FORM AS APPLIED IN THE TEST: solid
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of activated sludge: Easton Wastewater Treatment Facility, Easton, Maryland on March 05, 2018, receiving predominantly residential wastes
- Preparation of inoculum for exposure: The sludge was sieved using a 2-mm screen, adjusted to approximately 1000 mg total suspended solids/L with mineral media and then aerated at test temperature until use. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 10 mg/L
- Based on:
- TOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium (per liter):
1 mL calcium chloride solution (2.75%)
1 mL of ferric chloride solution (0.025%)
1 mL of magnesium sulfate solution (2.25%)
10 mL of phosphate buffer (pH 7.4)
- Test temperature: 19.15-21.69 ºC
- pH: 7.48 - 7.60
- pH adjusted: no
- Suspended solids concentration: ≤ 30 mg/L
TEST SYSTEM
- Culturing apparatus: Amber 4-L bottles, 3-L fill volume. The air entering the chambers was passed through Drierite™ to remove ambient moisture and then through Ascarite® to produce CO2-free air.
- Number of culture flasks/concentration: 3
- Details of trap for CO2 and volatile organics if used: The air exiting the test chambers was passed through a series of three gas washing bottles, each containing approximately 100 mL of 0.5 M potassium hydroxide (KOH) to trap the CO2 that had evolved within the chamber. On sampling days, the CO2 trap nearest the test chamber was collected for analysis of inorganic carbon. The two remaining traps were placed one position closer to the test chamber and a new trap was placed on the end of the series.
SAMPLING
- Sampling frequency: On days 2, 5, 8, 12, 15, 18, 22, and 26
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: yes
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 17.9
- Sampling time:
- 28 d
- Details on results:
- The toxicity control achieved > 25% degradation by Day 14 (62.9%) and therefore MTDID 10628 may be considered non-inhibitory at the concentration tested in this study. The amount of CO2 evolved by the blank control chambers (24.3 mg/L) did not exceed the 40 mg/L value considered the acceptable limit for CO2 evolution tests.
BOD5 / COD results
- Results with reference substance:
- An average of 104.3% of theoretical CO2 was evolved over the test period; an average biodegradation greater than 60% was achieved by Day 5.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- reference >60% degraded by day 14, difference in test substance degradation between chambers <20%, total CO2 in blanks <40 mg/L (24.3 mg/L)
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- 1,1'-Isophthaloylbis(2-methylaziridine) was degraded 17.9% in an OECD 301B test. 1,1'-Isophthaloylbis(2-methylaziridine) was not inhibitory to biodegradation.
- Executive summary:
The degradability of 1,1'-isophthaloylbis(2-methylaziridine) was assessed according to OECD 301B. The test contained a blank control group, a reference group (sodium benzoate), and a treatment group, each with three replicates and a single toxicity control. the reference substance was degraded adequately by day 5. The average cumulative percent biodegradation for 1,1'-isophthaloylbis(2-methylaziridine) was 17.9% by the end of the 28-day test. The toxicity control was biodegraded 62.9% in the same time period. The test substance is not readily biodegradable but is not inhibitory to biodegradation.
The test was conducted according to internationally accepted guidelines under GLP criteria. It is considered reliable without restrictions and is suitable for Risk Assessment, Classification & Labelling, and PBT Analysis.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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