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Diss Factsheets
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EC number: 201-353-5 | CAS number: 81-48-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: EEC Method No. L 133/118 -122
- Version / remarks:
- Edition : 30.05.1988
- Deviations:
- yes
- Remarks:
- see 'Principles of method if other than guideline'
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- April 4, 1984
- Deviations:
- yes
- Remarks:
- see 'Principles of method if other than guideline'
- Principles of method if other than guideline:
- Deionized water (instead of dest. water for the stock solution); Preparation of the inoculum: higher sludge weight after the washing procedure (Sludge concentrations in the test according to the guidelines); 4 instead of 5 test concentrations
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Purity approx. 95 %
- Code number: FAT - 40504/A
- Test No.: 19-div.
- Appearance: solid
- Storage: room temperature
- Expiration date: 12/98 - Analytical monitoring:
- no
- Details on sampling:
- Test substance
- Storage: room temperature - Vehicle:
- yes
- Details on test solutions:
- Since the test substance swam at the surface of the medium, the stock solution was prepared by adding one drop of the emulsifier Tween 80 (concentration 26.6 mg/L). The highest solvent concentrations used in the test (17.02 mg/L) was additionally tested as solvent control.
Stock solution
The stock solution was treated 20 min. in a ultrasonic bath for dispergation. - Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Origin: Activated sludge of a communal sewage treatment plant (ARA - Pro Rheno Basel, Switzerland)
- Sludge pretreatment: 3 times washing with tap water and addition of synthetic sewage (a day before the main test)
- Sludge dry weight: 1.56 g/L
- pH of sludge: 7.8 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 21.5°C
- pH:
- 7.7 (concerning the highest test concentration of 320 mg/L after 3 hours)
- Dissolved oxygen:
- 4.5 mg/L (concerning the highest test concentration of 320 mg/L after 3 hours)
- Nominal and measured concentrations:
- Test substance: 320, 100, 32 and 10 mg/L (nominal)
Reference substance (3,5-Dichlorophenol): 32, 10 and 3.2 mg/L - Details on test conditions:
- PRE-TREATMENT OF THE STOCK SOLUTION:
- The stock solution was treated 20 min. in an ultrasonic bath for dispergation
TEST CONDITIONS:
- Test concentrations: 4 (10, 32, 100 and 320 mg/L)
- Concentrations of the reference substance: 3 (3.2, 10, 32 mg/L)
- Temperature: 21.5 °C
- Test volume: 100 mL
- Test duration: 3 h
- Estimation technique: O2 - consumption, measured with an oxygen sensitive electrode system: OXI DIGI 530 (SAV: 00 109)
- Aeration: 0.1 - 0.2 L/min by pasteur-pipette
- pH of sludge: 7.8 before the test beginning
SOLVENT CONTROL:
- Tween 80 (Fluka No. 93781) - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol (Merck 821768)
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- The solvent (Tween) had no inhibitory effect on activated sludge respiration activity. After 3 hours the inhibition of the respiration rate was 2.5 % for the highest solvent concentration used in the test (17.2 mg/L).
- Results with reference substance (positive control):
- 3,5-Dichorophenol: 3h-EC50 12.5 mg/L (test concentrations: 32,10 and 3.2 mg/l)
- Validity criteria fulfilled:
- yes
- Remarks:
- respiratory rates of the 2 controls differ less than 15 % from each other; EC50 of 3,5-Dichlorophenol is in the range 2 - 25 mg/L
- Conclusions:
- Solvent Violet 13 was exposed to activated sludge of domestic sewage treatment plant and an 3h-IC50 of > 320 mg/L was obtained. The effect value related to nominal concentration.
- Executive summary:
A study was performed to assess the toxicity of Solvent Violet 13 to microorganisms. The study was conducted according to the EEC Method No. L 133/118 -122 (Edition : 30.05.1988). The activated sludge was exposed to 4 test concentrations (10, 32, 100 and 320 mg/L). Since the substance swam at the surface of the medium, the stock solution was prepared by adding one drop of the emulsifier (Tween 80). The highest solvent concentrations used in the test (17.02 mg/L) was additionally tested as solvent control and an inhibitory effect on activated sludge respiration activity could not be detected. The respiration rate was determined after aeration periods of 3 hours. The IC50 of Solvent Violet 13 was higher than 320 mg/L. The effect value related to nominal concentration, since no analytical monitoring was performed. This toxicity study is classified as acceptable and satisfies the guideline requirements for the toxicity study to microorganisms.
Reference
Solvent control
Since the test substance swam at the surface of the medium, the stock solution was prepared by adding one drop of the emulsifier Tween 80 (concentration 26.6 mg/l).
The highest solvent concentrations used in the test (17.02 mg/l) was additionally tested as solvent control.
IC50
The respiration rate of each test concentration was calculated as a percentage of the mean of the two control respiration rates. The results were plotted on a logarithmic (concentration) / normal (% inhibition) graph, where the IC50 can be evaluated.
Description of key information
A study was performed to assess the toxicity of Solvent Violet 13 to microorganisms according to the EEC Method No. L 133/118 -122 (Edition : 30.05.1988). The activated sludge was exposed to 4 test concentrations (10, 32, 100 and 320 mg/L). Since the substance swam at the surface of the medium, the stock solution was prepared by adding one drop of emulsifier (Tween 80). The highest solvent concentrations used in the test (17.02 mg/L) was additionally tested as solvent control and an inhibitory effect on activated sludge respiration activity could not be detected. The respiration rate was determined after aeration periods of 3 hours. The IC50 of Solvent Violet 13 was higher than 320 mg/L. The effect value related to nominal concentration, since no analytical monitoring was performed.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 320 mg/L
Additional information
"Should read: IC50 > 320 mg/L"
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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