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EC number: 500-209-1 | CAS number: 68412-54-4 1 - 2.5 moles ethoxylated
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 08 to 23 March 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study conducted according to EU guideline 84/449/EC in compliance with GLP. Analytical certificate not included in study report.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: EU guideline 84/449/EC of 19/09/1984
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Nonylphenol, branched, ethoxylated
- EC Number:
- 500-209-1
- EC Name:
- Nonylphenol, branched, ethoxylated
- Cas Number:
- 68412-54-4
- Molecular formula:
- not available for this UVCB
- IUPAC Name:
- Nonylphenol, branched, ethoxylated (> 1 < 2.5 mol EO)
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Borchem, Germany
- Age at study initiation: 9 (males) or 10 (females) weeks
- Weight at study initiation: 172 (males) or 173 (females) g
- Fasting period before study: 16 to 4 h before test begin
- Housing: Makrolon Type III cages
- Diet (e.g. ad libitum): Fixed formula standard diet Altromin 1324 pellets (Altromin GmbH)
- Water (e.g. ad libitum): tap water
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 2°C
- Humidity (%): 50 +/- 10%
- Air changes (per hr): 10
- Photoperiod (hrs dark / hrs light): 12 / 12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- polyethylene glycol
- Details on oral exposure:
- VEHICLE
- Amount of vehicle (if gavage): 10 ml/kg bw - Doses:
- 1,000 (only females), 2,000, 3,100 and 5,000 mg/kg bw
- No. of animals per sex per dose:
- 5 males and 5 females
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Several times on the day of dosing then twice daily until test end: type, onset, duration and intensity of symptoms and mortality. Predosing, after one week and at test end: bodyweight
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight - Statistics:
- According to Rosiello AP et al. (1977), J. Tox. Environ. Health 3:797, modified by Pauluhn J (Bayer AG, report no. 11835, 1983). Based on maximum likelihood method of Bliss CI (1938), Q. J. Pharm. Pharmacol. 11:92.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 5 000 mg/kg bw
- Based on:
- test mat.
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
Any other information on results incl. tables
As of 3,100 (males) and 2,000 (females) mg/kg bw, observed symptoms included poor general condition, rough fur, sedation, unconsciousness, side or stomach position, paralysis-like effects in the posterior extremities. High gait was seen in males at 5,000 mg/kg bw and females at 2,000 and 5,000 mg/kg bw. Bloody nose and weight loss were observed in females at 2,000 mg/kg bw. In one female at 3,100 mg/kg bw, there was shortness of breath.
In males at 5,000 mg/kg bw and females as of 3,100 mg/kg bw which died before test end, gross macroscopical examination revealed damaged mucous membrane of the stomach, redenned stomach fundus, as well as redenned and swollen gastro-intestinal tract. One male and 2 females at 5,000 mg/kg bw also displayed redenned lungs.
There were no symptoms at 2,000 (males) and 1,000 (females) mg/kg bw.
Applicant's summary and conclusion
- Conclusions:
- Under the conditions of the study, the acute oral LD50 of the substance was 5,000 mg/kg bw for males and > 2,000 mg/kg bw for females.
- Executive summary:
A study was conducted to determine the acute oral toxicity of NPEO to rat in accordance with EU Guideline 84/449/EC of 19/09/1984. Male and female Wistar rats received the test substance diluted in polyethylene glycol via gavage at doses of 1,000 (females only), 2,000, 3,100 and 5,000 mg/kg bw. Mortality and clinical signs were recorded throughout the study. Gross macroscopy was conducted at the end of the test period. As of 3,100 (males) and 2,000 (females) mg/kg bw, symptoms included poor general condition, rough fur, sedation, unconsciousness, side or stomach position, paralysis-like effects in the posterior extremities. High gait was seen in males at 5,000 mg/kg bw and females at 2,000 and 5,000 mg/kg bw. Bloody nose and weight loss were observed in females at 2,000 mg/kg bw. In one female at 3,100 mg/kg bw, there was shortness of breath. In males at 5,000 mg/kg bw and females as of 3,100 mg/kg bw which died before test end, gross macroscopical examination revealed damaged mucous membrane of the stomach, redenned stomach fundus, as well as redenned and swollen gastro-intestinal tract. One male and 2 females at 5,000 mg/kg bw also displayed redenned lungs. Under the conditions of the study, the acute oral LD50 of the substance was 5,000 mg/kg bw for males and > 2,000 mg/kg bw for females (Ivens-Kohl I, 1988).
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