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EC number: 233-215-5 | CAS number: 10081-67-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Endpoint summary
Administrative data
Description of key information
Aquatic toxicity is discussed. The substance is not considered to be hazardous to aquatic organisms at the limit of solubility in water.
Additional information
Various tests are available to assess the toxicity of the substance to environmental organisms. The results of these tests are as follows:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Based on the results of the test (given the poor solubility of the test substance in water, the limit test was used), the following value was established:
the highest concentration causing no mortality in fish
Based on the results of the acute toxicity tests in Poecilia reticulata fish(limit test), the following was established for the test substance : the test substance in concentration of <= 100 mg/l (test substance in suspension) does not cause mortality in Poecilia reticulata during a 96 h test. In accordance with ECHA Guidance document, Chapter R.7b – Endpoint Specific Guidance, “Figure R. 7.8-5: Considerations for difficult substances” the limit value of 100 mg/l is taken as the definitive value for hazard classification purposes.
The substance is not considered to be harmful to fish at the limit if concentration in water. No classification is applicable.
OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)
Results based on nominal conc. of 4-(1-methyl-1-phenylethyl)-N-[4-(1-methyl-1-phenylethyl)phenyl]aniline
Endpoint: Hatch: NOEC: ≥50 μg/L
Endpoint: Survival (post-hatch) NOEC: ≥50 μg/L
Endpoint: Growth (length) NOEC: ≥50 μg/L
Endpoint: Growth (wet weight) NOEC: ≥50 μg/L
Endpoint: Growth (dry weight) NOEC: ≥50 μg/L
The overall NOEC, based on nominal concentrations of 4-(1-methyl-1-phenylethyl)-N-[4-(1-methyl-1-phenylethyl)phenyl]aniline at Day 28 post-hatch was therefore ≥50 μg/L. No toxicity was observed at the limit of solubility in water.
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
The test substance n the geometric series of test concentrations – 100 mg.l-1 and less - did not immobilise any Daphnia magna, therefore, the EC(IC)50 at the intervals of 24 and 48 hours could not be established. Based on the test results, an additional value was established:
the highest concentration causing no immobilisation or mortality of Daphnia magna
Based on the results of acute toxicity tests with Daphnia magna (limit test), it was established for the test substance that the test substance in concentration of <= 100 mg.l-1 (test substance in suspension) does not cause immobilisation of any Daphnia magna. In accordance with ECHA Guidance document, Chapter R.7b – Endpoint Specific Guidance, “Figure R. 7.8-5: Considerations for difficult substances” the limit value of 100 mg/l is taken as the definitive value for hazard classification purposes.
The substance is not considered to be harmful to Daphnia at the limit if concentration in water. No classification is applicable.
OECD Guideline 211 (Daphnia magna Reproduction Test)
The cladoceran, Daphnia magna, was exposed to the test substance at mean measured concentrations of 1.9, 4.5, 11.4, 21 and 38 μg a.i/L under flow-through conditions for 21 days. There were no significant treatment-related effects on survival, reproduction or growth at concentrations ≤38 μg a.i./L. Consequently, the NOEC, based on growth, was 38 μg a.i./L. The LOEC was >38 μg a.i./L and the MATC was calculated to be >38 μg a.i./L. The 21-day EC50 value for adult immobility was >38 μg a.i./L, the highest concentration tested. The 21-day EC10 and EC50 values for reproduction based on mean number of live neonates produced per reproductive day were both >38 μg a.i./L. The 21-day EC10 value for reproduction based on mean number of live neonates produced per adult at the beginning of the test was 16 μg a.i./L. The 21-day EC50 value for reproduction based on mean number of live neonates produced per adult at the beginning of the test was >38 μg a.i./L.
OECD Guideline 201 (Alga, Growth Inhibition Test)
Key study:
Results; growth rate; yield
NOEC 13.0
LOEC >13.0
ErC50 >13.0
ErC20 >13.0
ErC10 >13.0
No effects were noted at the limit of solubility in water. In accordance with ECHA Guidance document, Chapter R.7b – Endpoint Specific Guidance, “Figure R. 7.8-5: Considerations for difficult substances” the limit value of 100 mg/l is taken as the definitive value for hazard classification purposes. The substance is not considered to be harmful to algae at the limit of concentration in water. No classification is applicable.
Supporting study:
A supporting study in Algae was rejected due to no analytical measurements. In this study, all other conditions of the test were met during the growth inhibition tests conducted on freshwater algae Selenastrum capricornutum. In the tests conducted on all controls(both the controls and ‘control A’s), the algal cell density (number of cells per ml) increased at least 103 x and the growth rate reached µ > 1.54 d-1; at the same time, pH changes did not exceed 1.38 units. Equally, the 72 h EbC(IbC)50 and 72 h ErC(IrC)50 values established for the reference substance - potassium dichromate (K2Cr2O7) comply with the required values, therefore, the results of the growth inhibition tests in freshwater algae Selenastrum capricornutum can be considered valid.
Given the fact that at test concentrations ≥ 25 mg/l , the test substance gradually separated from the solution during the tests despite the application of an auxiliary substance(acetone p.a.), it can be assumed that the values for the growth inhibition of algae Selenastrum capricornutum for the individual concentrations do not correspond with the actual concentration of the test substance DUSANTOX® 86 in the solution, therefore, the 72 h EbC(IbC)50 and 72 h ErC(IrC)50 values may be inaccurate; in any case, the 72 h EbC(IbC)50 and 72 h ErC(IrC)50 values for the test substance DUSANTOX® 86 (for all five samples) are > 10 mg/l.
In accordance with ECHA Guidance document, Chapter R.7b – Endpoint Specific Guidance, “Figure R. 7.8-5: Considerations for difficult substances” the limit value of 100 mg/l is taken as the definitive value for hazard classification purposes. The substance is not considered to be harmful to algae at the limit of concentration in water. No classification is applicable.
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
The test substance was not completely soluble in test medium at the loading rates initially prepared. Therefore, the test substance – Milli-RO water mixtures were magnetically stirred for a period of approximately 25 hours. Subsequently, synthetic medium, sludge and Milli-RO water were added resulting in the required loading rates. Optimal contact between the test substance and test medium was ensured applying continuous aeration and stirring during the 3-hour exposure period.
The test substance was not toxic to waste water (activated sludge) bacteria at or below a loading rate of 1000 mg/l (NOEC). The EC50 exceeded a loading rate of 1000 mg/l.
On the basis of these results it is considered that the test substance does not pose a hazard to aquatic organisms at the limit of solubility in water. No further testing is required to refine risk.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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