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Diss Factsheets
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EC number: 205-355-7 | CAS number: 139-13-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP and guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- testing lab.
Test material
- Reference substance name:
- Trilon A
- IUPAC Name:
- Trilon A
- Reference substance name:
- Trisodium nitrilotriacetate
- EC Number:
- 225-768-6
- EC Name:
- Trisodium nitrilotriacetate
- Cas Number:
- 5064-31-3
- IUPAC Name:
- trisodium 2,2',2''-nitrilotriacetate
- Details on test material:
- purity 93%
impurities: < 7% water; < 3% sodium glycolate; 2% disodium irninodi(acetate); < 2% sodium hydroxide; < 1.5% methanamine; < 1 % sodium formate
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Vehicle:
- other: water
- Details on inhalation exposure:
- The objective of this study in rats was to provide data on the sensory irritation of Trisodium Nitrilotriacetate (NTA) by means of the Alarie test in Wistar rats. Although the sensory irritation test onginally was developed for mice, section 7.1.3 of guideline ASTM E981-04 (see below) indicates that the same system can be used with guinea pigs or rats. In addition, by using rats in the sensory irritation test, it will be easier to
link these data to data obtained from acute and subacute inhalation toxicity studies with NTA. In addition, mice are much better able to reduce their minute breathing volume and cope with the increased blood CO2 concentration than either rats or humans. Data from rats may therefore be more relevant for extrapolation to humans than data from mice. - Duration of exposure:
- 30 min
- Concentrations:
- 0.91 mg/L (low concentration group), 2.86 mg/L (mid concentration group) and 4.25 mg/L (high concentration group)
- Control animals:
- yes
Results and discussion
Effect levels
- Sex:
- male
- Dose descriptor:
- other: RD50
- Effect level:
- 4.25 mg/L air
- Exp. duration:
- 30 min
Any other information on results incl. tables
Exposure to the test material at all concentrations resulted in a change in breathing pattem characterised by an irregular breathing pattem. These consisted of normal breathing intermingled with periods of irregular breathing. However, post-inspiratory apnoeas, characteristic for sensory imtation, were only observed in one out of four animals exposed to 2.86 mg/L and in two out of four animals exposed to 4.25 mg/L. Clinical signs were not seen during the 7-day post-exposure period. Mean body weight gain was low from day 0 to day 3, whereas from day 3 to day 7 body weight gain was considered to be within a normal range for animals of this strain and age. Absolute and relative lung weights of the exposed animals were considered to be normal for animals of this strain and age. Abnormalities were not observed at necropsy 7 days after exposure.
Overall, the results of this study show sensory imtation to be present in rats at levels of 2.86 and 4.25 mg/L NTA. The RD50 value was estimated to be close to 4.25 mg/L. Although there was high inter-individual variability in responses amongst the rats, these results suggest that sensory irritation is confined to high exposure levels, close to the technically highest attainable concentration. Sensory imtation was absent at a level of 0.91 mg/L NTA.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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