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EC number: 287-476-5 | CAS number: 85535-84-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- no data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Modified guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- yes
- Remarks:
- NH4Cl omitted from medium (to prevent nitrification); fewer bottles/treatment than recommendation
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- yes
- Remarks:
- NH4Cl omitted from medium (to prevent nitrification); fewer bottles/treatment than recommendation
- GLP compliance:
- yes
Test material
- Reference substance name:
- Alkanes, C10-13, chloro
- EC Number:
- 287-476-5
- EC Name:
- Alkanes, C10-13, chloro
- Cas Number:
- 85535-84-8
- IUPAC Name:
- Alkanes, C10-C13, Chloro
- Details on test material:
- - Name of test material (as cited in study report): Cereclor 50LV
- Substance type: technical product
- Physical state: clear liquid
- Analytical purity: approx 100%
- Impurities (identity and concentrations): no data
- Composition of test material, percentage of components: C10-13 chlorinated n-alkanes (50% chlorinated)
- Isomers composition: no data
- Purity test date: no data
- Lot/batch No.: 716984
- Expiration date of the lot/batch: no data
- Stability under test conditions: no data
- Storage condition of test material: no data
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
none
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: secondary activated sludge, domestic (non adapted) and river water
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
- (i) Nieuwgraaf domestic wastewater treatment plant, Duiven, The Netherlands.
- (ii) River water was sampled from the Rhine near Heveadorp, The Netherlands
- Preparation of inoculum for exposure: River water and activated sludge (400 mg dryweight/l) were preconditioned by aeration for 1 week
- Concentration of sludge: 2 mg dry weight/l
- Initial cell/biomass concentration: no data
- Water filtered: no - Duration of test (contact time):
- 56 d
Initial test substance concentration
- Initial conc.:
- 2 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: KH2PO4 (8.5 mg/l); K2HPO4 (21.75 mg/l); Na2HPO4.2H2O (33.4 mg/l); MgSO4.7H2O (22.5 mg/l); CaCl2 (27.5 mg/l); FeCl3.6H2O (0.25 mg/l) in deionised water
- Additional substrate:
- Solubilising agent (type and concentration if used): silicone oil AR20 (2 ml/l); polyalkoxylate alkylphenol (2 ml/l)
- Test temperature: 22-24oC
- pH: (i) activated sludge on day 0 - 7.0; (ii) river water day 0 - 8.0
- pH adjusted: no
- CEC (meq/100 g): no data
- Aeration of dilution water: no data
- Continuous darkness: no data
- Other:
TEST SYSTEM
- Culturing apparatus: 0.3L BOD bottles
- Number of culture flasks/concentration: 3
- Measuring equipment: oxygen electrode/meter - WTW Trioxmatic EO 200/WTW Oxi 530
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: yes
STATISTICAL METHODS: none
Reference substance
- Reference substance:
- not specified
Results and discussion
- Preliminary study:
- none
- Test performance:
- no data
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 72
- Sampling time:
- 28 d
- Remarks on result:
- other: activated sludge; Cereclor 50 LV emulsion with agnique
- Parameter:
- % degradation (O2 consumption)
- Value:
- 47
- Sampling time:
- 56 d
- Remarks on result:
- other: activated sludge; Cereclor 50 LV added in DCM
- Parameter:
- % degradation (O2 consumption)
- Value:
- 75
- Sampling time:
- 42 d
- Remarks on result:
- other: activated sludge; Cereclor 50LV added in agnique
- Parameter:
- % degradation (O2 consumption)
- Value:
- 37
- Sampling time:
- 56 d
- Remarks on result:
- other: river water; Cereclor 50LV added in DCM
- Parameter:
- % degradation (O2 consumption)
- Value:
- 64
- Sampling time:
- 56 d
- Remarks on result:
- other: river water; Cereclor 50LV added in agnique
- Details on results:
- % degradation at 28 days when added in DCM - 36% (activated sludge) and 18% (river water)
% degradation at 28 days when added in agnique - 72% (activated sludge) and 42% (river water)
% degradation at 28 days when added in silicone oil - 7% (activated sludge) and 10% (river water)
% degradation at 42 days when added in agnique - 75% (activated sludge) and 63% (river water)
% degradation at 28 days when added in DCM - 47% (activated sludge) and 37% (river water)
% degradation at 56 days when added in silicone oil - 25% (activated sludge) and 38% (river water)
% degradation at 56 days when added in agnique - 64% (river water).
The biodegradation of the C10 -13 chlorinated paraffin (50% chlorinated) reached the pass level (>60% within a 10-day window in no more than 28 days), indicating ready biodegradability when incubated as an emulsion with activated sludge.
The test material did not inhibit endogenous respiration by either inoculum.
BOD5 / COD results
- Results with reference substance:
- none
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- oxygen concentration >0.5 mg/l in all bottles during the test
- Interpretation of results:
- other: only readily biodegradable when introduced as an emulsion in activated sludge
- Conclusions:
- In a closed bottle ready biodegradability test, carried out using a modified OECD Guideline 301D/EU Method C.4-E approach, the biodegradation of Cereclor 50LV (a C10-13 chlorinated paraffin; 50% chlorination) after 28 days by activated sewage was shown to be between 7 and 72% and by river water, 10-42%, depending on the method of introduction of the test material into the incubation medium (volatile solvent, silicone oil, surfactant solution). Biodegradation in excess of 60% was only obtained with test material emulsified with surfactant (72% at 28 days with activated sludge and 63% at 42 days with river water).
- Executive summary:
In a closed bottle biodegradability test (considered the most relevant test for poorly-soluble compounds), conducted according to a modified OECD Guideline 301D/EU Method C.4 -E, the biodegradation of Cereclor 50LV (a C10-13 chlorinated paraffin; 50% chlorination) was assessed by measuring oxygen consumption during incubation with either secondary activated sewage sludge or river water for up to 56 days. The test material was introduced in to the incubation medium at a nominal concentration of 2 mg/L either as a thin film on the bottle surface deposited from a volatile solvent (dichloromethane), as a solution in silicone oil or as an emulsion in a polyalkoxylate surfactant (agnique).
The biodegradation of the test material by activated sludge at 28 days was 36, 7 and 72% after introduction by volatile solvent, silicone oil and as an emulsion, respectively. The corresponding values for incubation in river water were 18, 10 and 42%, respectively. The biodegradation of test material introduced as an emulsion increased to 75% by day 42 with activated sludge and 63 and 64% by days 42 and 56 respectively in river water. The biodegradation of solvent-introduced material by 56 days was 47 and 37% in activated sludge and river water respectively , the corresponding values following introduction in silicone oil were 25 and 38% respectively. The test material did not inhibit endogenous respiration by either inoculum.
The biodegradation of the C10 -13 chlorinated paraffin (50% chlorinated) reached the pass level (>60% within a 10-day window in no more than 28 days), indicating ready biodegradability when incubated as an emulsion with activated sludge.
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