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Diss Factsheets
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EC number: 425-950-7 | CAS number: 187393-00-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to reproduction: other studies
Administrative data
- Endpoint:
- toxicity to reproduction: other studies
- Remarks:
- in vitro
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- In vitro androgen competitive binding assay
- GLP compliance:
- no
- Type of method:
- in vitro
Test material
Constituent 1
Administration / exposure
- Details on study design:
- Rat prostates were used as a source of cytosolic androgen receptors (AR).
Ten 8 week-old male Alpk: APfSD rats were each given a single subcutaneous injection of the GnRH antagonist Antarelix dissolved in hydroxypropyl methoxycellulose (0.3mg/kg). The animals were killed 24h later by an overdose of Fluothane followed by cervical dislocation, and ventral prostates were removed.
Finely minced prostates were homogenised in "low salt" buffer (1 gm of tissue per 10ml buffer) consisting of Tris (10 mM), glycerol (10% v/v), sodium molybdate (1 mM), EDTA (1.5 mM), dithiothreitol (1 mM) and phenylmethylsulphonyl fluoride (1mM), pH 7.4. The homogenate was centrifuged at 30,000g for 0.5h at 4° to yield the cytosol fraction.
The assay entailed the incubation of prostate cytosol (200 µl) with 3H-R1881 (0.19μCi; = 5x l0^-9M) and the required dose level of the putative competitor in DMSO (10 μl), all in a final volume of 500 μl, for 17h at 4°C. Serial, ten-fold dilutions of each compound (positive controls and test agents) were made in DMSO. These concentrations were so arranged as to give the specified molarity when 10 µl of each dosing preparation was diluted to 500 μl.
Two separate experiments were conducted. Both experiments contained a range of ten-fold dilutions of each test agent (5x10^-10 to 5x10^-4 M) and of R1881 (5x10^-10 to 5x10^-6 M) and testosterone (5x10^-10 to 5x10^-6 M in Experiment 1 and 5x10^-10 to 5x10^-7M in Experiment 2). Each experiment consisted of duplicate incubations of all the putative androgen receptor competitors and also of incubations with solvent alone (DMSO) to ascertain the 100% binding value. Duplicate incubations o 3H-R1881 in low salt buffer (i.e. in the absence of cytosol) gave a measure of non-specific binding.
The receptor-ligand complex was isolated at the termination of each incubation by the addition of hydroxylapatite (250μl of 60% v/v in Tris buffer [50mM]). Each precipitate was collected by centrifugation and washed three times with Tris buffer (50mM), collecting by centrifugation after each wash. The washed precipitates were suspended and the radioactivity determined in a Liquid Scintillation Analyser.
Results and discussion
Observed effects
In contrast, the test substance gave no indication of activity at any of the dose level tested.
Any other information on results incl. tables
Experiment 1:
CGF-C1607 binding % compared to solvent control (DMSO)
5x10^-10M: 96.8%
5x10^-9M: 97.3%
5x10^-8M: 100.2%
5x10^-7M: 98.8%
5x10^-6M: 99.8%
5x10^-5M: 98.2%
5x10^-4M: 93.6%
Testosterone (5 x10^-6): 9.8%
R1881 (5 x10^-6): 9.9%
Experiment 2:
CGF-C1607 binding % compared to solvent control (DMSO)
5x10^-10M: 101.7%
5x10^-9M: 99.9%
5x10^-8M: 102.6%
5x10^-7M: 102.6%
5x10^-6M: 100.8%
5x10^-5M: 102.0%
5x10^-4M: 100.7%
Testosterone (5 x10^-7): 13.4%
R1881 (5 x10^-6): 12.0%
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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