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Diss Factsheets
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EC number: 425-950-7 | CAS number: 187393-00-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to soil microorganisms
Administrative data
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 0015986251
- Expiration date of the lot/batch: 09.09.2021
- Purity test date: not indicated
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: ambient at room temperature
- Stability under test conditions: given
- Purity: 100 %
- water solubility: insoluble
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- no
- Details on preparation and application of test substrate:
- The test substance was added to the test medium directly mixed with quartz sand.
Test organisms
- Test organisms (inoculum):
- soil
Study design
- Total exposure duration:
- 28 d
Test conditions
- Test temperature:
- 20.8 - 22.3 °C
- Moisture:
- Water content: 5.1 g/100g dw (mean value of 2 values)
- Details on test conditions:
- TEST SYSTEM
- Testing facility: BASF SE
- Test container (type, material, size): 2 L glass container with a wide opening, overed with perforated aluminium lid
- Amount of soil: approx. 1147 g
- Amount of quartz sand: approx. 10 g
- No. of replicates per concentration: 1
- No. of replicates per control: 1
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
Soil type: 2.3
Batch no.: F2.3 0917
Sampling depth: Approximately 20 cm
Sampling quantity: Approximately 1000 kg
Sampling date: 01 Mar 2017
Weather condition on sampling: Rainfall with air temperature of 7 °C
Preparation of the soil by the supplier: Drying for 5 days at room temperature, Presieving
with 10 mm screen, Last sieving 2 mm screen.
Supplier: Landwirtschaftliche Untersuchungs- und Forschungsanstalt (LUFA) Speyer, Obere Langgasse 40, 67346 Speyer, Germany.
Arrival in the testing facility: 09 Mar 2017
Organic carbon: 0.68 ± 0.04%
Nitrogen content: 0.08 ± 0.01%
Sand (particle size >0.063 -2.0 mm): 57.2 ± 2.4 % (German DIN)
Soil type according German DIN: Silty sand (uS)
pH-value (0.01M CaCl2): 5.84 ± 0.6
Cation exchange capacity: 7.5 ± 0.8 meq/100g
Water holding capacity: 35.4 ± 1.0 g/100g
Grain size: 2 mm
Dry weight of the soil (measured): 95.2 % (mean value of 2 values)
Water content of the soil (measured): 5.1 g/100g Dw (mean value of 2 values)
Initial microbiological biomass (measured): 426.4 ± 20.0 mg carbon/kg Dw (mean value of 3 values, correspond to 6.3% of carbon content of the soil)
NITROGEN SOURCE:
Luzerne meal
Supplier: Tock GmbH Futtermühle; 66798 Wallerfangen-Ihn, Weinbachstr. 18-20
Date of delivery: 18 June 2003
Crude protein: 17 %
Carotene: 80 mg/kg
Content of carbon: 41.9 g/100g
Content of nitrogen: 2.4 g/100g
C/N ratio: 17/1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : measurement of nitrigen content after 7, 14 and 28 d.
VEHICLE CONTROL PERFORMED: no
- Nominal and measured concentrations:
- Nominal: 0, 62.5, 125, 250, 500, 1000 mg/kg dw
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 28 d
- Dose descriptor:
- EC10
- Effect conc.:
- >= 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Reported statistics and error estimates:
- The statistical evaluation of the data was carried out using ToxRat. If the % inhibition was lower than 0 % it was set to 0 %. If the %inhibition was greater than 100 % it was set to 100 %. A curve was fitted between the concentration and the % inhibition via the probit model. This curve was used for the estimation of the EC10, EC25 and EC50. Appropriate weighting for the model fitting via linear transformation were used. The confidence intervals were determined via normal approximation.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- No inhibitory effect son the nitrogen formation up to the highest test concentration of 1000 mg/kg soil dry weight.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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