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Diss Factsheets
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EC number: 201-297-1 | CAS number: 80-62-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 006
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 011
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Version / remarks:
- As described in: Kimber I, Basketter D A. The murine local lymph node assay: a commentary on collaborative studies and new directions. Food Chem Toxicol 1992: 30: 165–169.
- GLP compliance:
- not specified
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Methyl methacrylate
- EC Number:
- 201-297-1
- EC Name:
- Methyl methacrylate
- Cas Number:
- 80-62-6
- Molecular formula:
- C5H8O2
- IUPAC Name:
- methyl 2-methylprop-2-enoate
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- MMA (99.96% pure) was supplied by Lucite International UK Ltd via Cassel Works, Cleveland, UK.
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA/Ca
- Sex:
- not specified
- Details on test animals and environmental conditions:
- - Young adult CBA/Ca mice (Harlan, Bicester, Oxfordshire, UK)
- Age: 8–12 weeks
- Housing: metal cages
- Food: SDS PCD pelleted diet; Special Diets Services Ltd, Witham, UK)
- Water: ad libitum.
-Temperature: 22 +/- 3°C
- Relative humidity: 50 +/-20%
- Lightning: 12 hr light/dark cycle.
Study design: in vivo (LLNA)
- Vehicle:
- other: acetone or acetone/olive oil (4:1 v/v)
- Concentration:
- 0, 10, 30, 50 75, and 100% (neat)
- No. of animals per dose:
- 4 per group
- Details on study design:
- Groups of mice were exposed topically on the dorsum of both ears to 25 µl of various concentrations of chemical, or to the same volume of vehicle alone, daily for 3 consecutive days. Five days after the initiation of exposure, all mice were injected intravenously via the tail vein with 20 µCi of [3H] methyl thymidine in 250 µl of phosphate buffered saline. Five hrs later, mice were killed and the draining auricular lymph nodes were excised and pooled for each experimental group. A single-cell suspension of lymph node cells (LNC) was prepared; approximately 12 hr later, incorporation of [3H]TdR was measured and a stimulation index relative to the concurrent vehicle-treated control value was derived.
- Positive control substance(s):
- other: 2,4-Dinitrochlorobenzene
- Statistics:
- The estimated concentration of chemical required inducing an SI of 3 relative to concurrent vehicle treated controls, or EC3 value, was derived by linear interpolation of dose–response data.
The EC3 value was calculated by interpolating between 2 points on the SI axis, one immediately above, and the other immediately below, the SI value of 3.
Results and discussion
- Positive control results:
- Positive control: 2,4-Dinitrochlorobenzene (DNCB), Vehicle: Acetone/Olive Oil):
Conc (% w/v) SI
0 1
0.01 1.4
0.025 2.2
0.05 4.0
0.1 9.8
0.25 16.2
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Value:
- 1.4
- Test group / Remarks:
- 10 % (w/v)
- Remarks on result:
- other: Vehicle: acetone
- Parameter:
- SI
- Value:
- 2.3
- Test group / Remarks:
- 30 % (w/v)
- Remarks on result:
- other: Vehicle: acetone
- Parameter:
- SI
- Value:
- 2
- Test group / Remarks:
- 50 % (w/v)
- Remarks on result:
- other: Vehicle: acetone
- Key result
- Parameter:
- SI
- Value:
- 4.4
- Test group / Remarks:
- 75 % (w/v)
- Remarks on result:
- other: Vehicle: acetone
- Key result
- Parameter:
- SI
- Value:
- 7.3
- Test group / Remarks:
- 100 % (w/v)
- Remarks on result:
- other: Vehicle: acetone
- Parameter:
- SI
- Value:
- 1.5
- Test group / Remarks:
- 10 % (w/v)
- Remarks on result:
- other: Vehicle: acetone/olive oil
- Parameter:
- SI
- Value:
- 1.5
- Test group / Remarks:
- 30 % (w/v)
- Remarks on result:
- other: Vehicle: acetone/olive oil
- Parameter:
- SI
- Value:
- 1.5
- Test group / Remarks:
- 50 % (w/v)
- Remarks on result:
- other: Vehicle: acetone/olive oil
- Key result
- Parameter:
- SI
- Value:
- 4.4
- Test group / Remarks:
- 75 % (w/v)
- Remarks on result:
- other: Vehicle: acetone/olive oil
- Key result
- Parameter:
- SI
- Value:
- 3.6
- Test group / Remarks:
- 100 % (w/v)
- Remarks on result:
- other: Vehicle: acetone/olive oil
- Key result
- Parameter:
- EC3
- Value:
- ca. 60
- Test group / Remarks:
- Vehicle: acetone
- Key result
- Parameter:
- EC3
- Value:
- ca. 90
- Test group / Remarks:
- Vehicle: acetone/olive oil
- Cellular proliferation data / Observations:
- see table below
Any other information on results incl. tables
Desintegrations per minute (dpm) | |||
Conc. (% w/v) | MMA, vehicle acetone | MMA, vehicle acetone/olive oil | DNCB/ pos. Ctrl., vehicle acetone/olive oil |
0 | 137 | 243 | 609 |
0.01 | ND | ND | 839 |
0.025 | ND | ND | 1352 |
0.05 | ND | ND | 2455 |
0.1 | ND | ND | 5971 |
0.25 | ND | ND | 9843 |
10 | 205 | 341 | ND |
30 | 312 | 363 | ND |
50 | 274 | 369 | ND |
75 | 605 | 513 | ND |
100 (neat) | 999 | 874 | ND |
Stimulation Index (SI) | |||
Conc. (% w/v) | MMA, vehicle acetone | MMA, vehicle acetone/olive oil | DNCB/ pos. Ctrl., vehicle acetone/olive oil |
0 | 1.0 | 1.0 | 1.0 |
0.01 | ND | ND | 1.4 |
0.025 | ND | ND | 2.2 |
0.05 | ND | ND | 4.0 |
0.1 | ND | ND | 9.8 |
0.25 | ND | ND | 16.2 |
10 | 1.5 | 1.4 | ND |
30 | 2.3 | 1.5 | ND |
50 | 2.0 | 1.5 | ND |
75 | 4.4 | 2.1 | ND |
100 (neat) | 7.3 | 3.6 | ND |
Applicant's summary and conclusion
- Interpretation of results:
- Category 1B (indication of skin sensitising potential) based on GHS criteria
- Conclusions:
- In this local lymphnode assay methyl methacrylate attained the criteria of Skin sens Category 1 B
- Executive summary:
- .In a mouse local lymphnode assay (Betts 2006) acc. to OECD 429 methyl methacrylate was tested at concentrations of 10; 30; 50; 75 and 100 % in two different vehicles (acetone and acetone/olive oil). EC50 values were 50 % (acetone) and 90 % (acetone/olive oil). In this study methyl methacrylate is a weak skin sensitizer and attained in this study the criteria of skin sens category 1 B.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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