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EC number: 201-159-0 | CAS number: 78-93-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- yes
- Remarks:
- ; incubation temperature was 18 to 21ºC, instead of constant temperature (± 1ºC)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- yes
- Remarks:
- ; incubation temperature was 18 to 21ºC, instead of constant temperature (± 1ºC)
- Qualifier:
- according to guideline
- Guideline:
- EPA OTS 796.3200 (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- yes
- Remarks:
- ; incubation temperature was 18 to 21ºC, instead of constant temperature (± 1ºC)
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic (adaptation not specified)
- Remarks:
- There are no details of pre-adaptation, therefore it can be assumed that were not pre-adapted to the test substance prior to testing.
- Details on inoculum:
- The supernatant fraction from the secondary clarifier was collected from Prospect Bay Wastewater Treatment Facility, Grasonville, Maryland on February 10, 1998. This fraction was filtered through glass wool to produce the inoculum, which was then aerated until used. 1.6 mL/L of inoculum was spiked to the test bottles.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 2 mg/L
- Based on:
- test mat.
- Initial conc.:
- 5 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
The following stock solutions used to prepare the test medium:
Potassium dihydrogen orthophosphate, KH2PO4 ..... 8.50 g
Dipotassium hydrogen orthophosphate, K2HPO4 ..... 21.75 g
Disodium hydrogen orthophosphate dihydrate, Na2HPO4 – 2 H2O ..... 33.40 g
Ammonium chloride, NH4CI ..... 0.50 g
Dissolve in high quality water and make up to 1 litre.
(b) Calcium chloride solution, CaCI2 ..... 27.50 g
Dissolve in high quality water and make up to 1 litre.
Magnesium sulfate, MgSO4-7 H2O ..... 22.50 g
Dissolve in high quality water and make up to 1 litre.
Ferric chloride, FeCI3 – 6 H2O ..... 0.25 g
Dissolve in high quality water and make up to 1 litre.
The test medium will contain the following standard reagent solutions per liter of high quality water:
1 mL of phosphate buffet solution, APHA, pH 7.2
1 mL of calcium chloride solution, APHA, 2.75%
1 mL of magnesium sulfate solution, APHA, 2.25%
1 mL of ferric chloride solution, APHA, 0.025%
- Test temperature: 18 to 21°C
TEST SYSTEM
- Culturing apparatus: 300-mL BOD bottles
- Number of culture flasks/concentration: 10
SAMPLING
- Sampling frequency: Dissolved oxygen measurements were performed on two test chambers from the control, reference and treatment groups on days 0, 7, 14, 21, and 28. - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- . Reported as sodium benzoate
- Test performance:
- The validity criteria were met:
reference substance: degradation of sodium benzoate had reached 84% by day 14
10 day window: degradation of MEK had reached 70% by day 7
Inhibitory effect: there had been no evidence of toxicity of test substance to inoculum, therefore it was not considered necessary to prepare extra bottles to test this. It is evident from the high rate of degradation achieved that MEK was not toxic to inoculum at the concentrations tested
oxygen depletion in inoculum blank: oxygen depletion was 0.1 mg/l DO by the end of the test, therefore within the acceptable limit of 1.5 mg/l DO. The DO in the test vessels decreased to 3 mg/L at day 28 for the 2 mg/L MEK concentration. THe 5 mg/L MEK concentration fell below 1 mg/L on day 7 and thus most likely below 0.5 mg/L by the end of the test, indicating too high test substance concentration. 12.2 mg ThOD/L at this concentration are above the recommended 10 mg ThOD/L in the OECD TG. - Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 70
- Sampling time:
- 7 d
- Remarks on result:
- other: 2 mg/L sample
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 98
- Sampling time:
- 28 d
- Remarks on result:
- other: 2 mg/L sample
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- >= 61
- Sampling time:
- 7 d
- Remarks on result:
- other: 5 mg/L sample
- Details on results:
- The 5 mg/L (12.2 mg ThOD/L) concentration achieved the maximum percent biodegradation possible based on the theoretical oxygen demand of the test substance and the amount of oxygen present in the test system. Degradation was therefore limited by the test system; however, it is evident from the results with bottles dosed at 2 mg/l that MEK is readily biodegradable.
- Results with reference substance:
- degradation of sodium benzoate had reached 84% by day 14
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Biodegradation values of 70% after 7 days and 98% after 28 days were determined for the substance using a relevant test method and in compliance with GLP. The result is considered to be reliable.
Reference
Description of key information
Biodegradation: 98% degradation in 28 days (OECD 301D)
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
Ready biodegradability was tested according to the Closed Bottle test (OECD 301D, EC C.4-E) and in compliance with GLP (Wildlife International Ltd., 1998, for Royal Dutch Shell plc). Nominal initial test substance concentrations were 2 mg/L and 5 mg/L, corresponding to 1.33 mg DOC/L or 4.88 mg ThOD/L and 3.33 mg DOC/L or 12.22 mg ThOD/L. Measured Day 0 solutions were 1.2 and 2.8 mg DOC/L. The test was performed with inoculum prepared by filtering through glass wool the supernatant fraction collected from the secondary clarifier of Prospect Bay Wastewater Treatment Facility, which was then aerated until used. The test substance at 2 mg/L and 5 mg/L achieved mean degradations of 70% and 61% by Day 7, and 98% and 57% by the end of the study at day 28 respectively.
The 5 mg/L concentration achieved the maximum percent biodegradation possible based on the theoretical oxygen demand of the test substance and the amount of oxygen present in the test system, even by Day 7. The viability of the inoculum and validity of the test were supported by the results of the reference substance, sodium benzoate, which attained 84% degradation by Day 14. The Day 7 results clearly show that the 10-day window criterion was met for the biodegradation of the test material. The substance is considered to be readily biodegradable under the conditions of the test. Please refer to the document attached to Section 13 for further discussion of the study.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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