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EC number: 204-260-8 | CAS number: 118-56-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
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- Endpoint summary
- Stability
- Biodegradation
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 1989 - 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: guideline test with GLP, reliability reduced from 1 to 2 as study is used for read-across
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-ethylhexyl salicylate
- EC Number:
- 204-263-4
- EC Name:
- 2-ethylhexyl salicylate
- Cas Number:
- 118-60-5
- Molecular formula:
- C15H22O3
- IUPAC Name:
- 2-ethylhexyl salicylate
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- The testing was made on three SPF albino female rabbits of the stock Mol: Russian from Møllegaard Breeding Centre ApS, Ejby, DK-4623 LL. Skensved. The rabbits weighed 2300-2600 g and were earmarked with National Wing Bands, During a pre-period of one week and throughout the experiment the rabbits were kept to single PPL cages, 45 × 55 cm, with perforated floor. The rabbits had free access to food pellets "Altromin 2123" from Chr. Petersen A/S, DK-4100 Ringsted and to fresh water. The room temperature was 21 ± 2 °C and the relative humidity 55 ±15%. The air was changed 6 times an hour, and light was on from 6 to 18 hrs. Declaration and analysis certificate of diet mixture weere documented. The rabbits were kept in animal room No. 1.
Test system
- Vehicle:
- other: Diethyl phthalate (DEP)
- Controls:
- other: right eyes remained untreated and served as control
- Amount / concentration applied:
- 50% (w/w)
- Duration of treatment / exposure:
- one hour (eyes were rinsed with 20 ml 0.9% sodium chloride solution after 1 hour examination)
- Observation period (in vivo):
- 1, 24, 48 and 72 hours after the treatment
- Number of animals or in vitro replicates:
- 3 females
- Details on study design:
- The day before testing both eyes of the animals were examined with an ophthalmoscope to ensure there were no eye defects or irritation. The examination was performed before and after instillation of oculogottae floureaceini (Ph.N,).
Only the left eyes were treated. The right eyes remained untreated and served as control.
About 0.1 ml of the diluted test article was placed in the left eye of each rabbit by gently pulling the lower lid away from the eyeball to form a cup into which the test substance was dropped. The lids were then gently held together for about one second. The eyes were examined and the grade of ocular reaction was recorded 1 hour later. 24 hour later an examination was performed before and after installation of oculoguttao flouresceini. After the examined the eyes were rinsed with 20 ml of a 0.9% sodium chloride solution.The eyes were also examined 48 and 72 hours after the treatment
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- other: 24-72hours
- Score:
- 0
- Max. score:
- 4
- Remarks on result:
- other: no positive reaction at any tested group
- Irritation parameter:
- iris score
- Basis:
- mean
- Remarks:
- for all animals
- Time point:
- other: 24-72hours
- Score:
- 0
- Max. score:
- 2
- Remarks on result:
- other: no positive response in all animals
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- other: 24-72hours
- Score:
- 0.1
- Max. score:
- 3
- Reversibility:
- fully reversible within: 72hours
- Remarks on result:
- other: Slight reactions of conjunctiva (redness) were seen in one rabbit at the 1- and 24-hour reading No reactions of the conjunctiva, iris or cornea were observed in any of the rabbits at the 48- and 72-hour readings.
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- other: 24-72hours
- Score:
- 0
- Max. score:
- 4
- Remarks on result:
- other: no positive response in all animals
- Irritant / corrosive response data:
- Slight reactions of conjunctiva (redness) were seen in one rabbit at the 1- and 24-hour reading. No reactions of the conjunctiva, iris or cornea were observed in any of the rabbits at the 48- and 72-hour readings.
- Other effects:
- no data
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Under the experimental condition, it can be concluded that 2-ethylhexyl salicylate in a 50% solution caused no eye irritation to rabbits.
- Executive summary:
The primary eye irritant effect of 2-ethylhexyl salicylate in a 50% solution was investigated according to the method recommended in the OECD Guideline No. 405"Acute Eye Irritation/Corrosion". About 0.1 ml of the diluted test article was placed in the left eye of each rabbit by gently pulling the lower lid away from the eyeball to form a cup into which tho test substance was dropped. The lids were then gently held together for about one second. The eyes were examined and the grade of ocular reaction was recorded 1 hour later. 24 hour later an examination was performed before and after installation of oculoguttao flouresceini. After the examined the eyes were rinsed with 20 ml of a 0.9% sodium chloride solution.The eyes were also examined 48 and 72 hours after the treatment. Slight reactions of conjunctiva (redness) were seen in one rabbit at the 1- and 24-hour reading. No reactions of the conjunctiva, iris or cornea were observed in any of the rabbits at the 48- and 72-hour readings.
Based on the description as above, test article in 50% solution did not cause positive eye response in rabbits.
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