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Diss Factsheets
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EC number: 203-906-6 | CAS number: 111-77-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Specific investigations: other studies
Administrative data
- Endpoint:
- endocrine system modulation
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Endocrine-disrupting activity of hydraulic fracturing chemicals and adverse health outcomes after prenatal exposure in male mice
- Author:
- Kassotis CD, Klemp KC, Vu DC, Chung-Ho L, Meng CX, Besch-Willliford CL, Pinatti L, Zoeller T, Drobnis EZ, Balise VD, Isiguzo CJ, Williams MA, Tillit DE, Nagel SC.
- Year:
- 2 015
- Bibliographic source:
- Endocrinology 156(12), 4458-73
- Reference Type:
- publication
- Title:
- Estrogen and Androgen Receptor Activities of Hydraulic Fracturing Chemicals and Surface and Ground Water in a Drilling-Dense Region.
- Author:
- Kassotis CD, Tillitt DE, Davis JW, Hormann AM, Nagel SC
- Year:
- 2 014
- Bibliographic source:
- Endocrinology, 155(3):897–90
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Measurement of potential for endocrine disrupting activity for five nuclear receptors using a reporter gene assay in human endometrial cancer cells. The hypothesis was that the chemicals used in oil and natural gas operations (including this substance) would exhibit antagonist activities for nuclear hormone receptors.
- GLP compliance:
- no
- Type of method:
- in vitro
- Endpoint addressed:
- toxicity to reproduction / fertility
Test material
- Reference substance name:
- 2-(2-methoxyethoxy)ethanol
- EC Number:
- 203-906-6
- EC Name:
- 2-(2-methoxyethoxy)ethanol
- Cas Number:
- 111-77-3
- Molecular formula:
- C5H12O3
- IUPAC Name:
- 2-(2-methoxyethoxy)ethanol
Constituent 1
- Specific details on test material used for the study:
- Source: Sigma Aldrich. No purity information.
Results and discussion
Any other information on results incl. tables
The results as interpreted from the graphical information presented are as follows:
POSITIVE CONTROL |
DEGME |
|||||
Concentrations in molar |
EC10 |
EC50 |
EC50/EC10 |
EC10 |
EC50 |
EC50/EC10 |
Anti-oestrogenic |
3x10-11 |
3x10-10 |
10 |
1x10-7 |
not reached |
>1000 |
Anti-progestogenic |
8x10-10 |
5x10-9 |
6.3 |
5x10-5 |
not reached |
>1000 |
Anti-androgenic |
2x10-7 |
2x10-6 |
10 |
4x10-6 |
not reached |
>25 |
Anti-androgenic* |
9x10-8 |
2x10-6 |
22 |
2x10-6 |
~1x10-4 |
~50 |
Anti-glucocorticogenic |
2x10-10 |
7x10-9 |
35 |
5x10-5 |
not reached |
|
Anti-thyroidogenic |
1x10-7 |
1x10-6 |
10 |
No effects seen |
*HepG-2 liver cancer cell line. All others Ishikawa cell lines
For DEGME, the EC50 was not seen for most end points up to the maximum tested concentration.
Applicant's summary and conclusion
- Conclusions:
- For a number of reasons, it is difficult to draw conclusions from this study. There are a number of shortcomings, which may be due to deficiencies in the reporting rather than inherent deficiencies in the method, although it is not a validated one, but it means that these results cannot be relied upon as evidence of endocrine effects.
- Executive summary:
In an in vitro study using cells transfected with gene reporters, 24 chemicals found in fracking formulations were individually assessed for possible endocrine antagonism effects. DEGME was found to have some activity towards androgen, estrogen, progesterone and thyroid receptors but not thyroid. However, the effects were weak and the dose response curves shallow. IC50 values were not reached for the any of the tests using the Ishikawa cell line up to the maximum tested concentration of 0.1mM and only just seen (IC50 ~0.1mM) for the anti-androgenic activity of the reporter transfected into HepG-2 liver cancer cell line. Overall, the reliability of this study as reported cannot be assessed for a number of reasons, but particularly because no information is provided on the number of replicates or concentrations tested and a complete lack of statistical information.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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