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EC number: 220-864-4 | CAS number: 2921-88-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Batch Number: RM 1946
Purity: 97.4% - Analytical monitoring:
- no
- Vehicle:
- yes
- Remarks:
- acetone
- Details on preparation and application of test substrate:
- - Method of mixing into soil: For each group, the dose was thoroughly mixed with a small pre-mix of soil and the vehicle allowed to evaporate off prior to incorporation with the main soil bulk. Water was gradually mixed with the treated soil to give a moisture content equivalent to 35% of the dry weight.
- Method of application to soil surface: The test substance was applied as solutions in acetone prepared on the day of dosing
- Controls: Negative control soil was treated with vehicle only
- Chemical name of vehicle: Acetone
- Evaporation of vehicle before use: Vehicle was allowed to evaporate before use - Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: Earthworm
- Source: Mr. D. Saunders, Fox Road, Bourn, Cambridgeshire, England
- Weight at test initiation: 300-337 mg
ACCLIMATION
- Acclimation period: One day - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 14 d
- Test temperature:
- 25-27°C
- Moisture:
- 34-35%
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): One-litre glass containers
- Amount of soil or substrate: 736 g wet weight
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per vehicle control: 4
PROPERTIES OF SUBSTRATE
- Quartz sand: 70% w/w
- Kaolin clay: 20% w/w
- Sphagnum peat: 10% w/w
- Calcium carbonate was added to the soil mixture to give a pH of approximately 5.9
OTHER TEST CONDITIONS
- Photoperiod: Continuous
- Light intensity: 440 lux
EFFECT PARAMETERS MEASURED: Mortality was assessed by testing the reaction of each worm to a mechanical stimulus at either end. Any worms visible on the soil surface were observed daily for behavioural or pathological signs. Mortalities, live worms and those unaccounted for were recorded. Weights of worms were recorded in treatment replicates immediately prior to treatment and on Days 7 and 14.
VEHICLE CONTROL PERFORMED: Yes - Nominal and measured concentrations:
- 0, 95, 171, 309, 556, 1000 ppm
- Reference substance (positive control):
- yes
- Remarks:
- The LC50 values of chloroacetamide to the earthworm were determined in a similar study conducted in the year 1991.
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 209.9 other: ppm
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% confidence limits 191.9-232.0 ppm
- Duration:
- 7 d
- Dose descriptor:
- LC50
- Effect conc.:
- 297.6 other: ppm
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% confidence limits 274.1-311.9 ppm
- Details on results:
- - Mortality at end of exposure period: At 309, 556, 1000 ppm, 100% mortality was observed. At 95 and 171 ppm, 3 and 8% mortality was observed, respectively.
- Changes in body weight: Weight loss was observed in all groups. This was greater in treated groups than in the control, with some evidence of a treatment-related trend. - Results with reference substance (positive control):
- The LC50 values of chloroacetamide to the earthworm were determined in a similar study and were as follows:
Day 7 LC50: 52 ppm (95% confidence limits 47-58 ppm)
Day 14 LC50: 37 ppm (confidence limits could not be reliably determined because the data consisted predominantly of 0% and 100% responses). - Validity criteria fulfilled:
- yes
- Conclusions:
- 7-day LC50 (earthworm): 297.6 ppm
14-day LC50 (earthworm): 209.9 ppm - Executive summary:
This study was designed to determine the acute toxicity (LC50) of the test substance to the earthworm in an artificial soil under laboratory conditions.
The study was conducted in compliance with OECD guideline 207 and EEC Directive 87/302/EEC, Part C.
Worms were randomly allocated to treatment, with four replicates often worms per treatment. The concentrations of the test substance were 0, 95, 171, 309, 556, 1000 ppm. On Days 7 and 14, the medium was emptied from the test containers and the worms removed for counting. The worms were observed for mortality, behavioural or pathological signs and weight.
At 309, 556, 1000 ppm, 100% mortality was observed. At 95 and 171 ppm, 3 and 8% mortality was observed, respectively. Weight loss was observed in all groups. This was greater in treated groups than in the control, with some evidence of a treatment-related trend.
Based on the distribution of mortalities the following LC50 values were calculated:
Day 7 LC50: 297.6 ppm (95% confidence limits 274.1-311.9 ppm)
Day 14 LC50 : 209.9 ppm (95% confidence limits 191.9-232.0 ppm)
Reference
Description of key information
7-day LC50 (earthworm): 297.6 ppm, 14-day LC50 (earthworm): 209.9 ppm; OECD 207, EU Method C.8; Reliability = 1
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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