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EC number: 448-300-4 | CAS number: 88642-03-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control and saturated solution
- Sampling method: Samples were taken from freshly
prepared media after 0, 24, 48, and 72 h and from 24 h old media after 24, 48, 72 and 96 h - Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The preparation of the saturated solution followed the procedures descirbed in the study "Alga, growth inhibition test with Desmodesmus subspicatus, 72 h" (study no.: SS0115151) (for details s. key study in section 6.1.5). The saturated solution was prepared with the column elution method (quartz sand; 2.1 mg test item/kg quartz sand). - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Source: AQUARIUM AM AEGI, Hannover, Germany
- Length at study initiation: Average body length at the test start: 2.50 cm
- Weight at study initiation: Average body weight at the test start: 0.22 g
- Method of breeding: Holding was performed at the testing facility at 23 ± 2 °C and diffuse light (0.1-10 µmol photons m^-2*s^-1, natural photoperiod). The water was changed at least once per week. The dissolved oxygen concentration was more than 80 % of the air saturation value.
ACCLIMATION
- Acclimation period: at least 12 days
- Acclimation conditions: same as test
- Type and amount of food during acclimation: Stör perlets; SERA GMBH, Heinsberg, Germany; 4 % of the fish body weight per feeding day
- Feeding frequency during acclimation: 3 times per week
- Health during acclimation: no mortality
FEEDING DURING TEST: none - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 57 - 65 mg CaCO3/L
- Test temperature:
- 23.1 - 24.0 °C
- pH:
- new medium: 7.2 - 8.1
old medium: 7.0 - 7.2 - Dissolved oxygen:
- new medium: 94 - 100 %
old medium: 61 - 78 % - Nominal and measured concentrations:
- Nominal 100% saturated solution
Geometric mean measured concentration: 279 µg/L - Details on test conditions:
- TEST SYSTEM
- Renewal rate of test solution: every 24 h
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water of local origin. water was filtered on activated charcola and aerated for at least 24 h to remove chlorine
- Culture medium different from test medium:no
- Intervals of water quality measurement: PH-value, temperature and oxygen saturation were measured in all vessels after beginning the test and every 24 h (from old and new media).
OTHER TEST CONDITIONS
- Photoperiod: natural photoperiod
- Light intensity: 0.1 - 10 µmol photons*m-2*s-1
EFFECT PARAMETERS MEASURED: Symtoms of toxicity and mortality after 2, 24, 48, 72 and 96 h
- Reference substance (positive control):
- not required
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 279 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 279 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: s. table
- Mortality of control: none
- Other adverse effects control: none - Sublethal observations / clinical signs:
Observation in the test vessels (n = 7)
Geometric mean
measured
concentration
[µg/L]Effect*
Test Duration [h]
2
24
48
72
96
(1)
-
-
5/7
5/7
6/7
279
(2.4)
7/7
7/7
2/7
2/7
1/7
(4.1)
7/7
-
-
-
-
Control
(1)
7/7
7/7
7/7
7/7
7/7
*) The number in brackets correspond to the following observation:
(1) = Normal behaviour (2.4) = Slow escape reflex (4.1) = Hyperventilation
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study the test item did not cause lethal effects to zebrafish after 96 h up to the solubility limit (saturation solution level under test conditions: 279 µg/L as geometric mean measured concentration)
Reference
Description of key information
The 96 -hour LC50 for Danio rerio > 279 µg/L (solubility limit).
Key value for chemical safety assessment
Additional information
The acute toxicity of the test item to Danio rerio was determined according to the principles of the OECD guideline 203 and in compliance with GLP. This test was carried out as a limit test under semi-static conditions with renewal of the test media every 24 hours. Seven test organisms were exposed to a saturated solution of the test item and the control. In this study the test item did not cause lethal effects to zebrafish after 96 h up to the solubility limit (saturation solution level under test conditions: 279 µg/L as geometric mean measured concentration).
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