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Diss Factsheets
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EC number: 613-953-8 | CAS number: 66603-10-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 971
- Report date:
- 1971
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Version / remarks:
- Before OECD guideline 405 was established, eye irritation was tested using an internal method (BASF test).
- Deviations:
- yes
- Remarks:
- see below
- Principles of method if other than guideline:
- - Principle of test: in vivo test with rabbits
- Short description of test conditions: were introduced into the conjunctival sac of the rabbit eye and distributed uniformly over the surface of the eye.
- Parameters analysed / observed: Local irritant effects - GLP compliance:
- no
- Remarks:
- GLP was not compulsory at the time the study was performed
Test material
- Reference substance name:
- Cyclohexylhydroxydiazene 1-oxide, potassium salt
- EC Number:
- 613-953-8
- Cas Number:
- 66603-10-9
- Molecular formula:
- C6H11KN2O2
- IUPAC Name:
- Cyclohexylhydroxydiazene 1-oxide, potassium salt
- Test material form:
- liquid
- Details on test material:
- Xyligen 30F is a 30% aqueous solution of K-HDO.
Constituent 1
- Specific details on test material used for the study:
- Xyligen 30 F has been applied undiluted.
Test animals / tissue source
- Species:
- rabbit
- Strain:
- Vienna White
- Details on test animals or tissues and environmental conditions:
- - Source: obtained commercially
- Sex: male / female
- Weight at study initiation: 2.75 kg (mean value)
- Diet: ad libitum
- Water: ad libitum
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent negative control
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): about 50 mm³
- Concentration (if solution): the substance has been applied undiluted.
VEHICLE
- no vehicle used - Duration of treatment / exposure:
- 8 days
- Observation period (in vivo):
- 1 hour, 24 hours, 8 days
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): washing is not mentioned in the report
SCORING SYSTEM:
- BASF scoring system: Explanation of signs:
0 = non-irritant
+/++ = slight / severe
R = reddening
E = edema
C = clouding of the cornea
S = scaling
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- conjunctivae score
- Remarks:
- Redness
- Basis:
- mean
- Time point:
- 24 h
- Score:
- 3
- Reversibility:
- fully reversible within: 8 days
- Irritation parameter:
- conjunctivae score
- Remarks:
- Chemosis
- Basis:
- mean
- Time point:
- 24 h
- Score:
- 4
- Reversibility:
- fully reversible within: 8 days
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 24 h
- Score:
- 3
- Reversibility:
- fully reversible within: 8 days
- Irritant / corrosive response data:
- The instillation of about 50 mm³ of the undiluted product into the conjunctival sac of the rabbit eye brought about marked reddening and relatively severe swelling of the conjunctiva, together with mucosal bleeding and blurred clouding of the cornea. The inflammatory changes were reversible over the course of 8 days.
- Other effects:
- No other effects observed.
Any other information on results incl. tables
The average score between 24h, 48h and 72h cannot be calculated because the values for 48h and 72h are not documented. Therefore, the 24h value has to be used instead. Since this 24h score of 1,5 is considered to correspond to an OECD score of 3 and since bleeding was observed also 24 hours after exposure the 30% K-HDO is considered to cause risk for serious damage to eye.
Applicant's summary and conclusion
- Conclusions:
- K-HDO 30 % in water is considered to cause serious damage to eyes.
- Executive summary:
The instillation of about 50 mm³ of the undiluted product into the conjuctival sac of the rabbit eye brought about marked reddening and relatively severe swelling of the conjunctiva, together with mucosal bleeding and blurred clouding of the cornea. The inflammatory changes were reversible over the course of 8 days.
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