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EC number: 221-831-7 | CAS number: 3248-91-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from Secondary literature
Data source
Referenceopen allclose all
- Reference Type:
- secondary source
- Title:
- Opinion of the Scientific Committee on Consumer Safety Basic Violet 2 COLIPA no B115
- Author:
- Scientific Committee On Consumer Products – SCCP
- Year:
- 2 011
- Bibliographic source:
- SCIENTIFIC COMMITTEE ON CONSUMER PRODUCTS – SCCP, COLIPA no B115, during 12th plenary meeting of 20 September 2011
- Reference Type:
- secondary source
- Title:
- oral toxicity study of Basic Violet 2 in the rat
- Author:
- Scientific Committee On Cosmetic Products And Non-Food Products (SCCNFP)
- Year:
- 2 002
- Bibliographic source:
- RTC (Research Toxicology Centre, Roma), 7121/T/213/99, October 02, 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: As mentioned below
- Principles of method if other than guideline:
- The Acute oral toxicity test of Basic Violet 2 (3248-91-7) was performed in Sprague Dawley Rat according to OECD guideline no. 401 (1987).
- GLP compliance:
- not specified
- Test type:
- other: No data available
- Limit test:
- yes
Test material
- Reference substance name:
- 4-[(4-amino-m-tolyl)(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-o-toluidine monohydrochloride
- EC Number:
- 221-831-7
- EC Name:
- 4-[(4-amino-m-tolyl)(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-o-toluidine monohydrochloride
- Cas Number:
- 3248-91-7
- Molecular formula:
- C22H24ClN3
- IUPAC Name:
- 4-[(4-amino-m-tolyl)(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-o-toluidine monohydrochloride
- Details on test material:
- - Name of test material (IUPAC name): 4-[(4-amino-m-tolyl)(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-o-toluidine monohydrochloride]
- Common name: LOWACRYL VIOLET 2
- Molecular formula: C22H24ClN3
- Molecular weight: 365.906 g/mol
- Smiles notation: C(\c1cc(c(N)cc1)C)(c1cc(c(N)cc1)C)=C1\C=C(C(=[NH+])C=C1)C.[ClH-]
- InChl: 1S/C22H23N3.ClH/c1-13-10-16(4-7-19(13)23)22(17-5-8-20(24)14(2)11-17)18-6-9-21(25)15(3)12-18;/h4-12,23H,24-25H2,1-3H3;1H
- Substance type: Organic
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report):Basic Violet 2
- Molecular formula:C22H23N3.HCl
- Molecular weight:365.91 g/mol
- Substance type:organic
- Physical state:Green powder
-Purity:83.7%
- Impurities (identity and concentrations):16.3 %
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- No data available
Administration / exposure
- Route of administration:
- oral: unspecified
- Vehicle:
- CMC (carboxymethyl cellulose)
- Remarks:
- 0.5% CMC aqueous solution
- Details on oral exposure:
- Details on exposure
VEHICLE
- Concentration in vehicle:500, 1000 and 2000 mg/kg
- Amount of vehicle (if gavage): No data available
- Justification for choice of vehicle: No data available
- Lot/batch no. (if required): No data available
- Purity: No data available
MAXIMUM DOSE VOLUME APPLIED: 2000 mg/kg
DOSAGE PREPARATION (if unusual): the test substance was soluble in CMC aqueous solution.
CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: no data available - Doses:
- 500, 1000 and 2000 mg/kg bw
- No. of animals per sex per dose:
- FOR LIMIT TEST:
Total: 10 animals
Females: 5 and Males: 5
FOR MAIN STUDY:
TOTAL: 30 animals
500 mg/kg bw = 5 males and 5 females
1000 mg/kg bw = 5 males and 5 females
2000 mg/kg bw = 5 males and 5 females - Control animals:
- not specified
- Details on study design:
- No data available
- Statistics:
- No data available
Results and discussion
- Preliminary study:
- LIMIT TEST STUDY:
Mortality:3 males died within 5 days of dosing.
Clinical signs: Piloerection, breathing difficulties, staining of fur, urine and faeces were observed
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: At 2000 mg/kg bw, 1 male and 1 female died within 48 hours of dosing. No mortalities were observed at 500, 1000 mg/kg dose levels.
- Mortality:
- At 2000 mg/kg bw, 1 male and 1 female died within 48 hours of dosing. No mortalities were observed at 500, 1000 mg/kg bw dose levels.
- Clinical signs:
- other: At 2000 mg/kg bw: piloerection, reduced activity, hunched posture, breathing difficulties, staining of fur, urine and faeces, swollen abdomen.
- Gross pathology:
- No data available
- Other findings:
- No data available
Applicant's summary and conclusion
- Interpretation of results:
- Category 5 based on GHS criteria
- Conclusions:
- The LD50 value was considered to be > 2000 mg/kg bw,when Sprague Dawley rats were treated with 4-[(4-amino-m-tolyl)(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-o-toluidine monohydrochloride (3248-91-7) orally.
- Executive summary:
In acute oral toxicity study, 5 males and 5 females Sprague Dawley rats per group were treated with 4-[(4-amino-m-tolyl)(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-o-toluidine monohydrochloride(3248-91-7) with dose level of 500,1000 and 2000 mg/kg bw.0.5% CMC aqueous solution was used as a vehicle.3 males died within 5 days of dosing. At 2000 mg/kg bw, 1 male and 1 female died within 48 hours of dosing. No mortalities were observed at 500, 1000 mg/kg bw dose levels.Clinical signs at 2000 mg/kg bw observed were piloerection, reduced activity, hunched posture, breathing difficulties, staining of fur, urine and faeces, swollen abdomen. Therefore, Lethal dose (LD50) value was considered to be >2000 mg/kg bw when rats were treated with 4-[(4-amino-m-tolyl)(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-o-toluidine monohydrochloride orally.
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