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EC number: 200-866-1 | CAS number: 75-37-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Inhalation: Acute Lethal Concentration (ALC) Test; rat; NOAEC = 66400 ppm (179280 mg/m3). Reliability = 2
Key value for chemical safety assessment
Acute toxicity: via inhalation route
Link to relevant study records
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: This study was selected as the key study because the information provided for the hazard endpoint is sufficient for the purpose of classification and labelling and/or risk assessment.
- Principles of method if other than guideline:
- Male rats were exposed to the test substance at 6.64, 17.52, 31.9, 38.3, and 45.75% for 4 hours and were observed for 14 day after treatment. Gross pathology was performed on surviving rats after 14 days.
- GLP compliance:
- not specified
- Test type:
- other: Acute Lethal Concentration (ALC) Test
- Limit test:
- no
- Species:
- rat
- Strain:
- other: Chr-CD
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: Not reported
- Weight at study initiation: 240-297 g
- Fasting period before study: No
- Housing: Not reported
- Diet (e.g. ad libitum): Not reported
- Water (e.g. ad libitum): Not reported
- Acclimation period: Not reported
ENVIRONMENTAL CONDITIONS
- Temperature (°C): Not reported
- Humidity (%): Not reported
- Air changes (per hr): Not reported
- Photoperiod (hrs dark / hrs light): Not reported - Route of administration:
- inhalation: gas
- Type of inhalation exposure:
- not specified
- Vehicle:
- other: unchanged (no vehicle)
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: Exposure chamber; no further details provided
- Exposure chamber volume: Not reported
- Method of holding animals in test chamber: Not reported
- Source and rate of air: Not reported
- Method of conditioning air: Not reported
- System of generating particulates/aerosols: The gas was regulated through a calibrated flowmeter into a mixing chamber. Regulated flows of air and/or oxygen were used as the carrier gas from the mixing chamber to the exposure chamber. For the exposure with an analytical concentration of 6.64% (v/v), only air was used as the carrier gas. Starting with the 17.52% exposure, the chamber oxygen concentrations were 16-17%, which can cause hypoxemic symptoms. All subsequent exposures had oxygen added in an amount sufficient to maintain a chamber oxygen concentration of ~20%.
- Treatment of exhaust air: Not reported
- Temperature, humidity, pressure in air chamber: Not reported
TEST ATMOSPHERE
- Brief description of analytical method used: Atmospheres were sampled at 30-minute intervals and analyzed by thermal conductivity gas chromatography. Concentrations were determined from a standard curve.
- Samples taken from breathing zone: yes - Analytical verification of test atmosphere concentrations:
- yes
- Remarks:
- Thermal conductivity gas chromatography
- Duration of exposure:
- 4 h
- Concentrations:
- 6.64, 17.52, 31.9, 38.3, and 43.75%. (66400, 175200, 319000, 383000, and 437500 ppm)
- No. of animals per sex per dose:
- 6 males per concentration
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: Not reported
- Frequency of weighings: Not reported
- Necropsy of survivors performed: yes - Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- > 43.75 other: % (437500 ppm)
- Exp. duration:
- 4 h
- Remarks on result:
- other: Mortality in 2/6 at 43.75% and 1/6 at 38.3%. At ≥ 17.52% lethargy, laboured breathing, reduced responsiveness to sound were observed. At 6.64% only hyperaemia and shallow breathing were observed.
- Mortality:
- Mortality ratio of 1/6 occurred at 38.3% (383000 ppm) and 2/6 occurred at 43.75% (437500 ppm).
- Clinical signs:
- other: Shallow breathing and hyperaemia were observed at 6.64%. During subsequent exposures additional signs observed included laboured breathing, lethargy, and unresponsiveness to sound were observed during exposure. Laboured breathing was more noticeable as
- Gross pathology:
- No gross changes were observed.
- Conclusions:
- The study and the conclusions which are drawn from it fulfil the quality criteria (validity, reliability, repeatability).
4-hour ALC = 38.3% (v/v) with oxygen added to maintain a chamber level of ca. 20% O2. - Executive summary:
Male rats were exposed to 6.64, 17.52, 31.90, 38.3, or 43.75% (v/v) (66400, 175200, 319000, 383000 or 437500 ppm) of the test substance for 4 hours and were observed for 14 days. At the end of 14 days all surviving rats were given a gross necropsy. The 4-hour ALC (approximate lethal concentration) was 38.3% (v/v; 383000 ppm) with oxygen added to maintain a chamber level of ~20%.
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- discriminating conc.
- Value:
- 179 280 mg/m³ air
Additional information
Toxicity Description: The substance has low inhalation toxicity with a rat 4-hour LC50 value of greater than 437500 ppm. During inhalation to substance concentrations of 175200 ppm and higher, acute toxicity was associated with laboured breathing, lethargy and reduced responsiveness to sound. All effects resolved quickly at termination of exposure. There was mortality in 1/6 animals and 2/6 animals in the 383000 and 437500 ppm exposure groups, respectively.
Dose-response relationship: The acute systemic toxicity effects were observed in a dose dependent manner, but resolved quickly after exposure. This substance is a gas, and tests to evaluate dermal and oral systemic toxicity were not feasible.
Dose Descriptor: NOAEC for significant systemic effects resulting from acute inhalation exposure was 6.64% (66400 ppm).
Justification for selection of acute toxicity – inhalation endpoint
Acute Lethal Concentration (ALC) Test, Reliability = 2
Justification for classification or non-classification
Based on the rat 4-hour LC50 greater than 437500 ppm (1181250 mg/m3) and the observations during the acute inhalation exposure, the substance does not need to be classified for acute toxicity according the EU Directive 67/548/EEC and EU Classification, Labelling and Packaging of Substances and Mixtures (CLP) Regulation (EC) No. 1272/2008.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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