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EC number: 200-875-0 | CAS number: 75-50-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Skin: BASF AG, 1979. Industrial hygiene orientating investigation, Report Nr.77/361, rabbits, 24 h occlusive, undiluted.
Skin: BASF AG, 1960, report Nr. VI/389, 24 /
48 / 72 h occlusive.
Eye: BASF AG, 1979. Industrial hygiene orientating investigation, Report
Nr. 77/361, Draize Test, rabbits, 100 µL single application.
Respiratory irritation: Gagnaire et al., 1989. Nasal Irritation and
Pulmonary Toxicity of Aliphatic Amines in Mice. Mice, range of exposure
17-70 ppm
Key value for chemical safety assessment
Skin irritation / corrosion
Link to relevant study records
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well documented report which meets basic scientific principles.
- Reason / purpose for cross-reference:
- reference to same study
- Principles of method if other than guideline:
- Method: BASF-Test. Two animals were treated for 3 min and 4 hours using occlusive conditions. An application site of 2.5x2.5 cm was covered with the liquid test substance. After the application the skin was washed with Lutrol (50%). The animals were observed for 8 days and skin changes were recorded daily. The report describes findings after 24 hours and at the end of the observation period (8 days). For a final evaluation, the findings after 48 and 72 hours from the raw data were taken into account.
- GLP compliance:
- no
- Species:
- rabbit
- Strain:
- Vienna White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 2.83 kg (mean) - Type of coverage:
- occlusive
- Preparation of test site:
- not specified
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: untreated skin site of the same animal
- Duration of treatment / exposure:
- 3 min and 4 h
- Observation period:
- 8 days
- Number of animals:
- 3 min exposure: 4
4 h exposure: 2 - Details on study design:
- TEST SITE
- Area of exposure: 2.5 x 2.5 cm
REMOVAL OF TEST SUBSTANCE
- Washing (if done): with Lutrol and Lutrol/water (1:1)
- Time after start of exposure: after the exposure (3 min and 4 h)
- Irritation parameter:
- erythema score
- Remarks:
- 3 min exposure
- Basis:
- mean
- Time point:
- 24/48 h
- Score:
- 2.9
- Max. score:
- 4
- Reversibility:
- not fully reversible within: 8 days. The application caused necrosis.
- Remarks on result:
- other: 72 h reading is missing.
- Irritation parameter:
- erythema score
- Remarks:
- 4 h exposure
- Basis:
- mean
- Time point:
- 24/48 h
- Score:
- 4
- Max. score:
- 4
- Reversibility:
- not reversible
- Remarks:
- The application caused necrosis.
- Remarks on result:
- other: 72 h reading is missing.
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- 24/48 h
- Score:
- 1.8
- Max. score:
- 4
- Reversibility:
- not fully reversible within: 8 days
- Remarks on result:
- other: 72 h reading is missing
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- 24/48 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- not fully reversible within: 8 days
- Remarks on result:
- other: 72 h reading is missing
- Irritation parameter:
- erythema score
- Remarks:
- 3 min and 4 h exposure
- Basis:
- mean
- Time point:
- 72 h
- Remarks on result:
- not measured/tested
- Irritation parameter:
- edema score
- Remarks:
- 3 min and 4 hours exposure
- Basis:
- mean
- Time point:
- 72 h
- Remarks on result:
- not measured/tested
- Irritation parameter:
- erythema score
- Remarks:
- 4 hours exposure
- Basis:
- animal #1
- Time point:
- 24/48 h
- Score:
- 4
- Max. score:
- 4
- Reversibility:
- not reversible
- Irritation parameter:
- erythema score
- Remarks:
- 4 hours exposure
- Basis:
- animal #1
- Time point:
- 72 h
- Remarks on result:
- not measured/tested
- Irritation parameter:
- erythema score
- Remarks:
- 4 hours exposure
- Basis:
- animal #2
- Time point:
- 24/48 h
- Score:
- 4
- Max. score:
- 4
- Reversibility:
- not reversible
- Irritation parameter:
- erythema score
- Remarks:
- 4 hours exposure
- Basis:
- animal #2
- Time point:
- 72 h
- Remarks on result:
- not measured/tested
- Irritation parameter:
- erythema score
- Remarks:
- 3 min exposure
- Basis:
- animal #1
- Time point:
- 24 h
- Score:
- 3
- Max. score:
- 3
- Reversibility:
- not reversible
- Irritation parameter:
- erythema score
- Remarks:
- 3 min exposure
- Basis:
- animal #1
- Time point:
- 48 h
- Score:
- 2
- Reversibility:
- not reversible
- Irritation parameter:
- erythema score
- Remarks:
- 3 min exposure
- Basis:
- animal #1
- Time point:
- 72 h
- Remarks on result:
- not measured/tested
- Irritation parameter:
- erythema score
- Remarks:
- 3 min exposure
- Basis:
- animal #2
- Time point:
- 24/48 h
- Score:
- 3
- Max. score:
- 3
- Reversibility:
- not reversible
- Irritation parameter:
- erythema score
- Remarks:
- 3 min exposure
- Basis:
- animal #2
- Time point:
- 72 h
- Remarks on result:
- not measured/tested
- Irritation parameter:
- erythema score
- Remarks:
- 3 min exposure
- Basis:
- animal #3
- Time point:
- 24/48 h
- Score:
- 4
- Max. score:
- 4
- Reversibility:
- not reversible
- Irritation parameter:
- erythema score
- Remarks:
- 3 min exposure
- Basis:
- animal #3
- Time point:
- 72 h
- Remarks on result:
- not measured/tested
- Irritation parameter:
- erythema score
- Remarks:
- 3 min exposure
- Basis:
- animal #4
- Time point:
- 24/48 h
- Score:
- 2
- Max. score:
- 2
- Reversibility:
- not reversible
- Irritation parameter:
- erythema score
- Remarks:
- 3 min exposure
- Basis:
- animal #4
- Time point:
- 72 h
- Remarks on result:
- not measured/tested
- Irritation parameter:
- edema score
- Remarks:
- 4 hours exposure
- Basis:
- animal #1
- Time point:
- 24/48 h
- Score:
- 2
- Max. score:
- 2
- Reversibility:
- not reversible
- Irritation parameter:
- edema score
- Remarks:
- 4 hours exposure
- Basis:
- animal #1
- Time point:
- 72 h
- Remarks on result:
- not measured/tested
- Irritation parameter:
- edema score
- Remarks:
- 4 hours exposure
- Basis:
- animal #2
- Time point:
- 24/48 h
- Score:
- 2
- Max. score:
- 2
- Reversibility:
- not reversible
- Irritation parameter:
- edema score
- Remarks:
- 4 hours exposure
- Basis:
- animal #2
- Time point:
- 72 h
- Remarks on result:
- not measured/tested
- Irritation parameter:
- edema score
- Remarks:
- 3 min exposure
- Basis:
- animal #1
- Time point:
- 24/48 h
- Score:
- 2
- Max. score:
- 2
- Reversibility:
- not reversible
- Irritation parameter:
- edema score
- Remarks:
- 3 min exposure
- Basis:
- animal #1
- Time point:
- 72 h
- Remarks on result:
- not measured/tested
- Irritation parameter:
- edema score
- Remarks:
- 3 min exposure
- Basis:
- animal #2
- Time point:
- 24 h
- Score:
- 2
- Max. score:
- 2
- Reversibility:
- not reversible
- Irritation parameter:
- edema score
- Remarks:
- 3 min exposure
- Basis:
- animal #2
- Time point:
- 48 h
- Score:
- 1
- Max. score:
- 2
- Reversibility:
- not reversible
- Irritation parameter:
- edema score
- Remarks:
- 3 min exposure
- Basis:
- animal #2
- Time point:
- 72 h
- Remarks on result:
- not measured/tested
- Irritation parameter:
- edema score
- Remarks:
- 3 min exposure
- Basis:
- animal #3
- Time point:
- 24/48 h
- Score:
- 2
- Max. score:
- 2
- Reversibility:
- not reversible
- Irritation parameter:
- edema score
- Remarks:
- 3 min exposure
- Basis:
- animal #3
- Time point:
- 72 h
- Remarks on result:
- not measured/tested
- Irritation parameter:
- edema score
- Remarks:
- 3 min exposure
- Basis:
- animal #4
- Time point:
- 24/48 h
- Score:
- 2
- Max. score:
- 2
- Reversibility:
- not reversible
- Irritation parameter:
- edema score
- Remarks:
- 3 min exposure
- Basis:
- animal #4
- Time point:
- 72 h
- Remarks on result:
- not measured/tested
- Interpretation of results:
- Category 1B (corrosive) based on GHS criteria
- Conclusions:
- Trimethylamine is corrosive to the skin due to the irreversibility of the effects within 8 days.
Classification: Skin Corr. 1B, H314: Causes severe skin burns and eye damage - Executive summary:
A BASF AG internal procedure (1979) was used as in following described: two animals were treated for 3 min and 4 hours using occlusive conditions. An application site of 2.5x2.5 cm was covered with the liquid test substance. After the application the skin was washed with Lutrol (50%). The animals were observed for 8 days and skin changes were recorded daily. The report describes findings after 24 hours and at the end of the observation period (8 days). For a final evaluation, the findings after 48 and 72 hours from the raw data were taken into account. The 3 min exposure caused severe erythema and in 1 animal parchment like necrosis after 24 h. At the end of the observation period of 8 days leathery necrosis was observed. This is considered to be a full thickness necrosis.
The 4 h exposure caused soft necrosis after 24 h in both animals. At the end of the observation period of 8 days leathery necrosis was observed. This is considered to be a full thickness necrosis. The 4 h exposure caused soft necrosis after 24 h in both animals. At the end of the observation period of 8 days leathery necrosis was observed. This is also considered to be a full thickness necrosis.
Reference
Mean erythema score after 24 and 48 h (72 h reading is missing); (animal1/2)
Exposure time | 24 h | 48 h | 72 h | mean |
||
3 min |
3/3/4/2 |
2/3/4/2 |
- |
2.5/3/4/2 |
||
4 h |
4/4 |
4/4 |
- |
4/4 |
Mean oedema score after 24 and 48 h (72 h reading is missing); (animal1/2)
Exposure time | 24 h | 48 h | 72 h | mean | ||
3 min | 2/2/2/2 | 2/1/2/2 | - | 2/1.5/2/2 | ||
4 h | 2/2 | 2/2 | - | 2/2 |
The 3 min exposure caused severe erythema and in 1 animal parchment like necrosis after 24 h. At the end of the observation period of 8 days leathery necrosis was observed. This is considered to be a full thickness necrosis.
The 4 h exposure caused soft necrosis after 24 h in both animals. At the end of the observation period of 8 days leathery necrosis was observed. This is considered to be a full thickness necrosis.
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (corrosive)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well documented report which meets basic scientific principles
- Reason / purpose for cross-reference:
- reference to same study
- Principles of method if other than guideline:
- Draize Test
- GLP compliance:
- no
- Species:
- rabbit
- Strain:
- Vienna White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 2.97 kg
- Diet: SSNIFF, ad libitum
- Water: tap water, ad libitum - Vehicle:
- unchanged (no vehicle)
- Controls:
- other: the adjacent eye served as saline-control.
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 100 µL - Duration of treatment / exposure:
- single application, eyes were not washd out
- Observation period (in vivo):
- 14 days
- Number of animals or in vitro replicates:
- 3
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- other: 24 h - 48 h - 72 h
- Score:
- 2.5
- Max. score:
- 4
- Reversibility:
- not reversible
- Remarks on result:
- other: Test substance caused necrosis
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- other: 24 h - 48 h - 72 h
- Score:
- 1
- Max. score:
- 2
- Reversibility:
- not fully reversible within: 14 days
- Remarks on result:
- other: ciliar injection
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- other: 24 h - 48 h - 72 h
- Score:
- 2
- Max. score:
- 3
- Reversibility:
- not fully reversible within: 14 days
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- other: 24 h - 48 h - 72 h
- Score:
- 2.4
- Max. score:
- 4
- Reversibility:
- not fully reversible within: 14 days
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Time point:
- 24 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- other: the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Time point:
- 24 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- other: the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #3
- Time point:
- 24 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- not reversible
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Time point:
- 48 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- other: the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Time point:
- 48 h
- Remarks on result:
- not determinable
- Remarks:
- the readings at 48 hours, 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #3
- Time point:
- 48 h
- Score:
- 3
- Max. score:
- 4
- Reversibility:
- not reversible
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Time point:
- 72 h
- Score:
- 3
- Max. score:
- 4
- Reversibility:
- other: the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Time point:
- 72 h
- Score:
- 3
- Max. score:
- 4
- Reversibility:
- other: the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #3
- Time point:
- 72 h
- Score:
- 3
- Max. score:
- 4
- Reversibility:
- not reversible
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Time point:
- other: 8 days
- Remarks on result:
- not determinable
- Remarks:
- the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Time point:
- other: 8 days
- Remarks on result:
- not determinable
- Remarks:
- the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #3
- Time point:
- other: 8 days
- Score:
- 4
- Max. score:
- 4
- Reversibility:
- not reversible
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Time point:
- 14 d
- Remarks on result:
- not determinable
- Remarks:
- the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Time point:
- 14 d
- Remarks on result:
- not determinable
- Remarks:
- the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #3
- Time point:
- 14 d
- Score:
- 4
- Max. score:
- 4
- Reversibility:
- not reversible
- Irritation parameter:
- iris score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 1
- Reversibility:
- other: the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- iris score
- Basis:
- animal #1
- Time point:
- other: 8 days
- Remarks on result:
- not determinable
- Remarks:
- the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- iris score
- Basis:
- animal #1
- Time point:
- 14 d
- Remarks on result:
- not determinable
- Remarks:
- the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- iris score
- Basis:
- animal #2
- Time point:
- 24 h
- Score:
- 1
- Reversibility:
- other: the readings at 48 h, 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- iris score
- Basis:
- animal #2
- Time point:
- 48 h
- Remarks on result:
- not determinable
- Remarks:
- the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- iris score
- Basis:
- animal #2
- Time point:
- 72 h
- Score:
- 1
- Reversibility:
- other: the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- iris score
- Basis:
- animal #2
- Time point:
- other: 8 days
- Remarks on result:
- not determinable
- Remarks:
- the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- iris score
- Basis:
- animal #2
- Time point:
- 14 d
- Remarks on result:
- not determinable
- Remarks:
- the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- iris score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 1
- Reversibility:
- other: the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- iris score
- Basis:
- animal #3
- Time point:
- other: 8 days
- Remarks on result:
- not determinable
- Remarks:
- the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- iris score
- Basis:
- animal #3
- Time point:
- 14 d
- Remarks on result:
- not determinable
- Remarks:
- the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 2
- Max. score:
- 2
- Reversibility:
- other: the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- other: 8 days
- Remarks on result:
- not determinable
- Remarks:
- the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- 14 d
- Remarks on result:
- not determinable
- Remarks:
- the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Time point:
- 24 h
- Score:
- 2
- Max. score:
- 2
- Reversibility:
- other: the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Time point:
- 48 h
- Remarks on result:
- not determinable
- Remarks:
- the readings at 48 hours, 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Time point:
- 72 h
- Score:
- 2
- Max. score:
- 2
- Reversibility:
- other: the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Time point:
- other: 8 days
- Remarks on result:
- not determinable
- Remarks:
- the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Time point:
- 14 d
- Remarks on result:
- not determinable
- Remarks:
- the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 2
- Max. score:
- 2
- Reversibility:
- other: the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #3
- Time point:
- other: 8 days
- Remarks on result:
- not determinable
- Remarks:
- the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #3
- Time point:
- 14 d
- Remarks on result:
- not determinable
- Remarks:
- the readings at 8 and 14 days were due to suppuration not possible
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 2
- Max. score:
- 3
- Reversibility:
- not reversible
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- other: 8 days
- Score:
- 3
- Max. score:
- 3
- Reversibility:
- not reversible
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- 14 d
- Score:
- 3
- Max. score:
- 3
- Reversibility:
- not reversible
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- 24 h
- Score:
- 2
- Max. score:
- 3
- Reversibility:
- not reversible
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- 48 h
- Score:
- 3
- Max. score:
- 3
- Reversibility:
- not reversible
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- 72 h
- Score:
- 3
- Max. score:
- 3
- Reversibility:
- not reversible
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- other: 8 days
- Score:
- 3
- Max. score:
- 3
- Reversibility:
- not reversible
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- 14 d
- Score:
- 3
- Max. score:
- 3
- Reversibility:
- not reversible
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- 24 h
- Score:
- 2
- Max. score:
- 3
- Reversibility:
- not reversible
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- 48 h
- Score:
- 3
- Max. score:
- 3
- Reversibility:
- not reversible
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- 72 h
- Score:
- 3
- Max. score:
- 3
- Reversibility:
- not reversible
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- other: 8 days
- Score:
- 3
- Max. score:
- 3
- Reversibility:
- not reversible
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- 14 d
- Score:
- 3
- Max. score:
- 3
- Reversibility:
- not reversible
- Interpretation of results:
- Category 1 (irreversible effects on the eye) based on GHS criteria
- Conclusions:
- The application of the test substance caused red-brown discoloration of the mucous and nictating membrane. After 24 h cliliar injection was noted in all 3 animals. After 8 days severe corneal opacity was noted in 1 animal. Due to purulent exsudate in the other two animals the corneal opacity score was not possible to determine.
Trimethylamine (aqueous solution): Skin Corr. 1B, H314: Causes severe skin burns and eye damage
Trimethylamine (gaseous form): Eye damage 1, H318: Causes serious eye damage - Executive summary:
A Draize test was performed on 3 rabbits (Vienna White) by BASF (1979). One single application was done with 100 µL, the eyes were not washed out afterwards. The test duration was 14 days. The application of the test substance caused red-brown discoloration of the mucous and nictating membrane. After 24 h ciliar injection was noted in all 3 animals. After 8 days severe corneal opacity was noted in 1 animal. Due to purulent exsudate in the other two animals the corneal opacity score was not possible to determine.
Reference
Table 1
Animal 1/2/3 | 24 h | 48 h | 72 h | 8 days | 14 days | ||||
A) corneal opacity | 2/2/2 | 2/x/3 | 3/3/3 | x/x/4 | x/x/4 | ||||
B) area | 4/4/4 | 4/x/4 | 4/4/4 | x/x/4 | x/x/4 | ||||
a = AxBx5 | 40/40/40 | 40/-/60 | 60/60/60 | -/-/- | -/-/- | ||||
Iris | 1/1/1 | 1/x/1 | 1/1/1 | x/x/x | x/x/x | ||||
b = Ax5 | 5/5/5 | 5/-/5 | 4/4/4 | -/-/- | -/-/- | ||||
Conjunctiva |
|
|
|
|
|
||||
A) erythema |
2/2/2 |
2/x/2 |
2/2/2 |
x/x/3 |
x/x/3 |
||||
B) chemosis |
2/2/2 |
2/3/3 |
2/3/3 |
3/3/3 |
3/3/3 |
||||
C) secretion |
1/1/1 |
3/3/3 |
3/3/3 |
3/3/3 |
3/3/3 |
||||
c = (A+B+C)x2 |
10/10/10 |
14/12/16 |
14/16/16 |
-/-/- |
-/-/- |
||||
|
|
|
|
|
|
||||
Sum=a+b+c |
55/55/55 | 59/12/81 | 79/81/81 | -/-/- | -/-/- |
x: reading due to suppuration not possible.
The application of the test substance caused red-brown discoloration of the mucous and nictating membrane. After 24 h cliliar injection was noted in all 3 animals. After 8 days severe corneal opacity was noted in 1 animal. Due to purulent exsudate in the other two animals the corneal opacity score was not possible to determine.
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (irreversible damage)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (irritating)
Additional information
Skin irritation
A BASF AG internal procedure (1979) was used as in following described: two animals were treated for 3 min and 4 hours using occlusive conditions. An application site of 2.5x2.5 cm was covered with the liquid test substance. After the application the skin was washed with Lutrol (50%). The animals were observed for 8 days and skin changes were recorded daily. The report describes findings after 24 hours and at the end of the observation period (8 days). For a final evaluation, the findings after 48 and 72 hours from the raw data were taken into account. The 3 min exposure caused severe erythema and in 1 animal parchment like necrosis after 24 h. At the end of the observation period of 8 days leathery necrosis was observed. This is considered to be a full thickness necrosis.
The 4 h exposure caused soft necrosis after 24 h in both animals. At the end of the observation period of 8 days leathery necrosis was observed. This is considered to be a full thickness necrosis.The 4 h exposure caused soft necrosis after 24 h in both animals. At the end of the observation period of 8 days leathery necrosis was observed. This is also considered to be a full thickness necrosis.
BASF AG investigated in 1960 the skin irritation as described: two animals were treated for 1, 5 min and 15 min with trimethylamine (application site of 2.5 x 2.5 cm²). After the application time the skin was washed with 50 % Lutrol. The animals were observed for 26 days and skin changes were recorded. The report describes findings after 24 hours, 48 hours and after 72 hours. The 1 min exposure caused marked oedema and haemorrhagic areas on the day of application which remained almost unchanged for 1 week, at the end of the observation period of 26 days, scar formation could be noted. The 5 and 15 min exposure the treatment resulted in a red-brown necrosis of leather-like consistency on the day of application, then development of a hard crust, and scar formation after 3 weeks. Additionally they tested a 20 % TMA solution in olive oil and the skin effects were less pronounced, this is indicating that the concentration of the liquid formulation which is used on the skin is of great importance to evaluate its potential irritation or corrosive character.
Eye irritation
For eye irritation a Draize test was performed on 3 rabbits (Vienna White) by BASF (1979). One single application was done with 100 µL, the eyes were not washed out afterwards. The test duration was 14 days. The application of the test substance caused red-brown discoloration of the mucous and nictating membrane. After 24 h ciliar injection was noted in all 3 animals. After 8 days severe corneal opacity was noted in 1 animal. Due to purulent exsudate in the other two animals the corneal opacity score was not possible to determine.
Respiratory irritation
In nasal irritation and pulmonary toxicity study of 20 aliphatic amines in mice, Gagnaire et al. exposed mice to airborne TMA to determine RD50 value (Gagnaire et al., 1989, see 7.9.3. Endpoint)). This value indicates 50% decrease in the respiratory rate and considered to be successfully used to predict safe industrial exposure. The onset of action of TMA was very rapid, ca. 30 sec. to 1 min. 61 ppm of TMA was determined as RD50 in mice. At the end of a 15 -min exposure period, the recovery of respiratory frequencies to the pre-exposure values was also rapid, ca.1 min. Due to the rapid recovery of respiratory frequency in mice, trimethylamine is considered to be moderately irritating to the upper respiratory airways.
Effects on skin
irritation/corrosion: corrosion
Effects on eye irritation: corrosion
Effects on respiratory irritation: irritation
Justification for classification or non-classification
For Trimethylamine Classification and labelling for irritation/corrosion is warranted according to the criteria of EU Directive 67/548/EEC and EU Classification, Labelling and Packaging of Substances and Mixtures (CLP) Regulation (EC) No. 1272/2008:
GHS Classification & Labeling for Trimethylamine (aqueous solution): Skin Corr. 1B, H314: Causes severe skin burns and eye damage, STOT SE 3, H335: May cause respiratory irritation.
GHS Classification & Labeling for Trimethylamine (gaseous form): Skin Irrit. 2 , Eye Dam. 1, H315: Causes skin irritation, H318: Causes serious eye damage, STOT SE 3, H335: May cause respiratory irritation.
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