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EC number: 244-815-1 | CAS number: 22174-70-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2008-04-29 to 2008-05-02
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP lab following OECD guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 431 (In vitro skin corrosion: Human Skin Model Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3,3'-[methylenebis(oxymethylene)]bisheptane
- EC Number:
- 244-815-1
- EC Name:
- 3,3'-[methylenebis(oxymethylene)]bisheptane
- Cas Number:
- 22174-70-5
- Molecular formula:
- C17H36O2
- IUPAC Name:
- 3,3'-[methylenebis(oxymethylene)]diheptane
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material (as cited in study report): 2-ethylhexylal
- Physical state: liquid
- Analytical purity: 99.82%
- Lot/batch No.: 0803181500
- Expiration date of the lot/batch: 2009-03-18
- Storage condition of test material: room temperature
Constituent 1
Test animals
- Species:
- human
- Strain:
- other: Episkin Standard Model
Test system
- Type of coverage:
- open
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
- Duration of treatment / exposure:
- 3, 60 and 240 minutes
- Observation period:
- 3 hours after end of exposure
Results and discussion
In vitro
Results
- Irritation / corrosion parameter:
- other: other: Cell Viability
- Value:
- > 35
- Remarks on result:
- other:
- Remarks:
- Basis: mean. Time point: 240 minutes. Reversibility: no data. Remarks: Following OECD Guideline 431: the cell viability is never below 35% even after 4 hours exposure. (migrated information)
Applicant's summary and conclusion
- Interpretation of results:
- study cannot be used for classification
- Remarks:
- Migrated information: non corrosive
- Conclusions:
- According to the obtained results (cell viability never below 35% for tested exposure time) and reporting to the UN and EU classification criterias for corrosive chemical categorisation, we can conclude that the organic solvent 2-ethylhexylal can be classified as non corrosive.
- Executive summary:
The objective of this study is to predict and classify the skin corrositivity potential of the chemical product 2-ethylhexylal according to chemical safety regulation, by assessment of its effect on a reconstructed human epidermis.
The principle of this test is based on the experience that corrosive chemicals show cytotoxic effect following short-term exposure on the stratum corneum of the epidermis. The test protocol does not provide adequate information on skin irritation. The assay was conducted according to the OECD guideline n° 431 "In vitro skin corrosion: Human Skin Model Test".
The Episkin is a highly differentiated and stratified epidermis validated model comprising the main basal, supra basal, spinous and granular layers and a functional stratum corneum obtained after a culture period. Its use for skin corrositivity testing involves topical application of the test material to the surface of the epidermis, and the subsequent assessment of its effects on cell viability.
The selected positive and negative reference chemicals are phosphate buffer saline and acetic acid glacial.
Corrositivity potential of 2-ethylhexylal involved topical application of test item to the surface of the reconstructed human epidermis and the subsequent assessment of its effect on cell viability. 10µl of the test item was applied topically to the epidermal model for 3, 60 and 240 minutes (3 epidermis units were used per test material, 2 for positive and 1 for negative controls and time exposure). At the end of the treatment, all residual test item and chemical controls were removed by thoroughly rinsing epidermis units with 25 ml of sterile phosphate buffered saline solution. The resulting cell viability was further assessed by incubating tissues for 3 hours at 37°C, 5% CO2 atmosphere with MTT (0.3 mg/ml) in assay medium. The basis of this test is that viable cells metabolize MTT into intra-cellular formazan crystals. The precipitated formazan was finally extracted using acidified isopropanol and quantified spectrophotometrically at 550 nm by comparison to a blank (acidified propanol).
According to the obtained results (cell viability never below 35% for tested exposure time) and reporting to the UN and EU classification criteria for corrosive chemical categorization, we can conclude that the organic solvent 2-ethylhexylal can be classified as non corrosive (OECD 431: non-corrosive to skin if the viability after 3 minutes exposure is greater than or equal to 50% and the viability after 1 hour exposure is greater than or equal to 15%).
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