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EC number: 232-380-0 | CAS number: 8011-86-7 This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 34905.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
Description of key information
EC50 = 100 mg/L
Key value for chemical safety assessment
- EC50 for freshwater plants:
- 100 mg/L
Additional information
From Section 13.5.3 of the EU Manual of Decisions dated July 2006:
Alternatives to the algae growth inhibition test with coloured substances
In the Annex VI of Directive 67/548/EEC an exemption clause is mentioned on algae growth inhibition tests with coloured substances. The clause emphasises that “where it can be demonstrated in the case of highly coloured substances that algal growth is inhibited solely as a result of a reduction in light intensity, then the 72h EC50 for algae should not be used as a basis for classification.”
In order to make a distinction between shading and toxic inhibition effects, in 1994 some modifications to the algae growth inhibition test, known as the ETAD (Ecological and Toxicological Association of Dyes and Organic Pigments Manufacturers) method (also known as the double flask method), were suggested. Advanced insights revealed that the ETAD method is too simplistic to allow evaluation of both toxic and light absorption effects of the coloured substances and should not be used any more.
Instead, a modified standard algae growth inhibition test, which applies the recommendations in the OECD Guidance Document on Testing and Assessment, number 23 (2000), is recommended for testing light absorbing substances The following adjustments to the standard algae growth inhibition test, Annex V method C.3 (or OECD guideline 201) have to be applied:
• The irradiation (light intensity) should be in the highest end of the range prescribed in the method C.3 (or (draft revised) OECD guideline 201): 120μE m-2 s-1 or higher.
• The light path should be shortened by reduction of the volume of the test solutions (in the range of 5 - 25 ml).
• Sufficient agitation (for example by moderate shaking) should be performed in order to obtain a high frequency of exposure of the algae to high irradiation at the surface of the culture.
For highly light absorbing substances, the modified standard algae growth inhibition test is not recommended. With these particular substances, a modified standardLemna-test (draft OECD guideline 221) is recommended. The following modification to the standardLemnatest has to be applied: the test has to be performed on a black, non-reflecting surface.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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