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EC number: 200-076-7 | CAS number: 51-03-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
- Endpoint:
- fish early-life stage toxicity
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 72-4 (Fish Early Life-Stage and Aquatic Invertebrate Life-Cycle Studies)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-(2-butoxyethoxy)ethyl 6-propylpiperonyl ether
- EC Number:
- 200-076-7
- EC Name:
- 2-(2-butoxyethoxy)ethyl 6-propylpiperonyl ether
- Cas Number:
- 51-03-6
- Molecular formula:
- C19H30O5
- IUPAC Name:
- 2-(2-butoxyethoxy)ethyl 6-propylpiperonyl ether
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Dead and live embryos were counted daily until hatching was complete. Hatching was deemed complete when no more than 5 unhatched viable embryos remained in any control or treatment level egg incubation cup (exposure day 4). The 31-day post-hatch larvae exposure was initiated when 40 live larvae were impartially selected from the surviving larvae in each incubation cup on study day 4 and placed into their respective exposure aquaria (40 organisms per replicate/80 organisms per treatment level). During the post-hatch exposure period, observations of larval survival, behaviour and appearance were made and recorded daily. Dead larvae were removed at the time of observation. Larval survival was estimated at least twice weekly. At 31 days post-hatch exposure (study termination), the percent larval survival was determined.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Description of method:
Immediately prior to test start, groups of sixty Fathead minnow eggs, less than 24 hours old, were put into each of 14 incubation cups and maintained at 24 °C. To initiate the test, these incubation cups were then placed into exposure aquaria containing either PB-200 at concentrations of 0.047, 0.094, 0.19, 0.38 and 0.75 mg PBO/L, a solvent control or a blank water control. Acetone was used as solvent at a maximum amount of 0.018 mL/L. The test concentrations were based on results from a preliminary range finding test. Each treatment group was comprised of two replicates and maintained under flow-through conditions. Hatching of embryos was deemed complete four days after test start and numbers hatched were recorded.
The post-hatching exposure phase of the study was then initiated by selecting forty live larvae from each of the incubation cups and placing these into their respective aquaria where they remained for 31 days. During this time, observations of larval survival, behaviour and appearance were recorded daily. Any dead larvae were removed at the time of observation. At termination of the exposure phase, surviving larvae were anesthetized and individually measured for length and weighed for wet weight.
Test aquaria maintained a test solution volume of 15 L and a flow of approximately 6.4 volume replacements every 24 hours.
Water temperature, pH and dissolved Oxygen were recorded daily in each aquarium. Analytical determinations for PB-200 concentration were made from samples taken from each replicate of each treatment group at the start of the study and thereafter on days 4, 11, 18, 21, 25, 28, 31 and 35 (end of the study).
Test guidelines no.:
US EPA 72-4
Relevant deviations from test guidelines:
None
Test organisms
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- Species/strain Fathead minnow (Pimephales promelas)
Source: Springborn brood stock
Wild caught: No
Age/size: At the start of the study, the test organisms were in the form of eggs less than 24 hours old
Kind of food: Live brine shrimp nauplii (Artemia salina)
Amount of food: Ad libitum
Feeding frequency: Once on day 4 of the study, three times daily thereafter until day 34
Post-hatch transfer time: Not applicable
Time to first feeding: 4 days
Feeding of animals during test: Larvae were not fed during the 24-hours prior to study termination
Treatment for disease within 2 weeks preceeding test: No
Handling of embryos and larvae
The surviving larvae were anesthetized with MS-222 (tricaine methane-sulfonate), measured and weighed individually to calculate the mean and standard deviation of total length and wet weight (OECD 210/212).
Study design
- Test type:
- flow-through
- Total exposure duration:
- 4 d
- Post exposure observation period:
- 4-day incubation period and a 31-day post-hatch exposure period
Test conditions
- Hardness:
- 32 - 40 mg/L as CaCO3
- Test temperature:
- 25 ± 1°C
- pH:
- 6.9 - 7.4
- Dissolved oxygen:
- 7.2-8.1 mg/L
- Details on test conditions:
- TEST SYSTEM
Test type: Flow-through
Renewal of test solution: 6.4 aquarium volumes per 24-hour period, with a 90% replacement time of approximately 9.0 hours
Volume of test vessels: 15 L
Volume/animal: 0.375 L/fish
Number of animals/vessel: 40/vessel
Number of vessels/ concentration: 2
Test performed in closed vessels due to significant volatility of TS: No
Aeration of dilution water: Yes. Chemical grade oxygen was gently bubbled via an airstone into the column containing the dilution water.
PREPARATION OF TS SOLUTION FOR POORLY SOLUBLE OR VOLATILE TEST SUBSTANCES
Dispersion: No
Vehicle: Acetone
Concentration of vehicle. 0.0018% (vol/vol)
Vehicle control performed. Yes. A 52% (vol/vol) acetone stock solution was prepared by diluting 259 mL acetone with distilled water to volume in a 500 mL flask
TEST ORGANISMS
Species/strain: Fathead minnow (Pimephales promelas)
Source: Springborn brood stock
Wild caught: No
Age/size: At the start of the study, the test organisms were in the form of eggs less than 24 hours old
Kind of food Live brine shrimp nauplii (Artemia salina)
Amount of food: Ad libitum
Feeding frequency: Once on day 4 of the study, three times daily thereafter until day 34
Post-hatch transfer time: Not applicable
Time to first feeding: 4 days
Feeding of animals during test: Larvae were not fed during the 24-hours prior to study termination
Treatment for disease within 2 weeks preceeding test: No
DILUTION WATER
Source: Well water supplemented with Town of Wareham well water
Alkalinity: 19 – 28 mg/L as CaCO3
Hardness: 32 - 40 mg/L as CaCO3
pH: 7.0 – 7.2
Oxygen content: 7.0 – 8.6 mg/L
Conductance: 130 - 140 µmhos/cm
Holding water different from dilution water: No
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16-hour light and 8-hour dark photoperiod
- Light intensity: Durotest Vitalite® fluorescent lights centrally located above the test aquaria at a range of 25 to 80 footcandles (270 to 860 lux).
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 35 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.18 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- not specified
- Basis for effect:
- not specified
- Duration:
- 35 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.42 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- not specified
- Basis for effect:
- not specified
- Details on results:
- Range finding test: Performed
Concentrations: 0.19, 0.38, 0.75, 1.5 and 3.0 mg PB-200 / L.
Number/ percentage of animals showing adverse effects: Mortality of 20, 20, 40, 30 and 100 % was observed in the 0.19, 0.38, 0.75, 1.5 and 3.0 mg PB-200 / L test concentrations, respectively. Partial loss of equilibrium was observed in two surviving fish exposed to the 0.75 mg/L treatment level and in one surviving fish exposed to 1.5 mg PB-200 / L.
Nature of adverse effects: Not applicable
RESULTS TEST SUBSTANCE
Initial concentrations of test substance: 0.047, 0.094, 0.19, 0.38 and 0.75 mg PBO/L
Actual concentrations of test substance: 0.040, 0.11, 0.18, 0.42 and 0.80 mg PBO/L
Effect data: The NOEC was determined to be 0.18 mg PBO/L and the LOEC was determined to be 0.42 mg PBO/L. Utilizing these data, the MATC for Task Force Blend PB-200 and Fathead minnow embryos and larvae was determined to be > 0.18 and < 0.42 mg total PBO/L with a geometric mean of 0.27 mg total PBO/L (see the table "Effect data" attached in background material).
Concentration/response curve: see the graphic "Concentration-response" attached in background materia
RESULTS OF CONTROLS
Number/percentage of animals showing adverse effects: see the table "Effect data" attached in background material.
Nature of adverse effects: Not applicable. - Reported statistics and error estimates:
- All statistical analyses were conducted at the 95 % level of certainty except in the case of Shapiro-Wilks and Bartlett’s Tests, in which the 99 % level of certainty is preferred for qualifying tests.
(Computer program by Gulley, D.D., Boetler, A.M. and Bergman, H.L. 1991 Toxtat Release 3.3 University of Wyoming, Laramie, Wyoming).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- NOEC 0.18 mg PBO/L
LOEC 0.42 mg PBO/L
Validity criteria can be considered as fulfilled, see "Validity criteria for fish tests according to OECD 210-212" attached in background material.
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