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EC number: 608-591-2 | CAS number: 31221-06-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guidelinestudy and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- 5-Diazo-2,4,6(1H, 3H, 5H)-pyrimidinetrione
- EC Number:
- 608-591-2
- Cas Number:
- 31221-06-4
- Molecular formula:
- C4H2N4O3
- IUPAC Name:
- 5-Diazo-2,4,6(1H, 3H, 5H)-pyrimidinetrione
- Details on test material:
- content 96.9 %, orange solid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 8-12 weeks
- Weight at study initiation: 158-197 g
- Fasting period before study: 16-24 hours
- Housing: group caged
- Diet ad libitum
- Water ad libitum
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22
- Humidity (%): 55
- Air changes (per hr): 10
- Photoperiod (hrs dark / hrs light) 12 / 12:
I
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: the test item was formulated in tap water with the help of 2 % Cremophor EL
- Details on oral exposure:
- the test item was tested using a stepwise procedure, each step using three animals of a single sex.
the dose level to be used as the starting dose should be that which is most likely to produce mortality in some of the dosed animals - Doses:
- starting dose: 2000 mg/kg bw; if mortality occur next dose is 300 mg/kg bw next steps would be 50 and 5 mg/kg bw
- No. of animals per sex per dose:
- 3
- Control animals:
- no
- Details on study design:
- the test item was tested using a stepwise procedure, each step using three animals of a single sex.
the dose level to be used as the starting dose should be that which is most likely to produce mortality in some of the dosed animals
absence or presence of compound-related mortality of the animals dosed at one step determined the next step.i.e.:
no further testing is needed
doseing of three additional animals, with the same dose
dosing of three additional animals at the next higher or the next lower dose level - Statistics:
- no
Results and discussion
Effect levels
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 300 - < 2 000 mg/kg bw
- Mortality:
- --2000 mg/kg bw:
3/3 time of death: 2 d - 7 d (2/3 had to be killed moribund and 1/3 died during the observation period)
--first trial: 300 mg/kg bw: 0/3
-- second trial: 300 mg/kg bw: 0/3 - Clinical signs:
- --2000 mg/kg bw
piloerection, abdominal posititon, temporary creeping gait, labored breathing, narrowed palpebral fissure, pallor cyanosis, sinken flanks, uncoordinated gait, poor general condition, decreased motility, and hunched posture
300 mg/kg bw
was tolerated with clinical signs - Body weight:
- 300 mg/ kg bw
was tolerated without effects on weight gain - Gross pathology:
- 2000 mg/kg bw:
cloudy and hemorrhagic change-in-contents of intestine, black discolored liver, hemorrhagic change-in-content of urinary bladder and pale kidneys
300 mg/kg bw
was tolerated without pathological findings - Other findings:
- no further data
Applicant's summary and conclusion
- Executive summary:
Diazobarbitursäure was tested for acute oral toxicity in female Wistar rats according to OECD TG 423.resulting in a LD50 ranging between 2000 and 300 mg/kg bw. 2000 mg/kg bw caused clear clinical signs of toxicity and the death of 3/3 rats within 7 days whereas 300 mg/kg bw was tolerated without any pathological findings and the animals survived until the end of the observation time.
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