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Diss Factsheets
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EC number: 200-751-6 | CAS number: 71-36-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Activity of human contact allergens in the murine local lymph node assay
- Author:
- Ryan CA et al.
- Year:
- 2 000
- Bibliographic source:
- Contact dermatitis,Vol.43, 95-102
- Reference Type:
- publication
- Title:
- Compilation of historical local lymph node data for evaluation of skin sensitization alternative methods
- Author:
- Gerberick GF et al
- Year:
- 2 005
- Bibliographic source:
- Dermatitis Vol 16, No 4, pp 157-202
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- GLP compliance:
- not specified
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Butan-1-ol
- EC Number:
- 200-751-6
- EC Name:
- Butan-1-ol
- Cas Number:
- 71-36-3
- Molecular formula:
- C4H10O
- IUPAC Name:
- butan-1-ol
- Test material form:
- liquid
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- other: CBA/J mice strain and CBA/Ca strain
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: CBA/J strain mice:Harlan Sprague Dawley, Inc., Indianapolis, IN or Jackson
Laboratories, Bar Harbor, ME; female
CBA/Ca strain mice: Harlan Olac Bicester, Oxfordshire, UK
- Females (if applicable) nulliparous and non-pregnant: [yes/no/not specified] not specified
- Age at study initiation:6-12 weeks
Study design: in vivo (LLNA)
- Vehicle:
- other: distilled water
- Concentration:
- 5%, 10% and 20% (v/v) in distilled water
- No. of animals per dose:
- n=5 (Laboratory A) or n=4 (Laboratory B and C)
- Details on study design:
- - groups of mice were treated 1x a day for 3 consecutive days on the dorsum of both ears with 25 µL of
1 of 3 concentrations of test material or with vehicle
- 5 days after initial treatment, all mice were injected intravenously, via the tail vein, with 250 µL PBS
containing 20 mCi of 3H-methylthymidine (3H-TdR; specific activity 5.0 Ci/mmol for Laboratory A, 2.0 Ci/mmol for Laboratories B and C, respectively).
- 5 h later, mice were euthanised and draining auricular lymph nodes were removed and pooled for each
individual mouse (Laboratory A) or for each experimental group (Laboratories B and C)
- single cell suspensions were prepared
- samples were centrifuged, resuspended in 1 mL 5% TCA and transferred to 10 mL of
scintillation cocktail
- 3H-TdR incorporation was measured by b-scintillation counting and expressed
as disintegrations per min (dpm) per individual mouse (Laboratory A) or as dpm per node for each experimental group (Laboratories B and C)
Stimulation indices (SI) for each experimental group were determined as the increase in 3H-TdR incorporation relative to concurrent vehicle treated control group. Positive: 3-fold or greater increase in proliferation, compared with the concurrent vehicle control, obtained at 1 or more test concentrations.
Results and discussion
In vivo (LLNA)
Resultsopen allclose all
- Key result
- Parameter:
- SI
- Value:
- 1
- Test group / Remarks:
- vehicle water
- Key result
- Parameter:
- SI
- Value:
- 1.6
- Test group / Remarks:
- 5% (v/v)
- Key result
- Parameter:
- SI
- Value:
- 1.2
- Test group / Remarks:
- 10% (v/v)
- Key result
- Parameter:
- SI
- Value:
- 1.4
- Test group / Remarks:
- 20% (v/v)
- Cellular proliferation data / Observations:
- vehicle water: 366 ± 44 mean dpm (± SEM)
5% (v/v) 1-butanol: 598 ± 157 mean dpm (± SEM)
10% (v/v) 1-butanol: 451 ± 17 mean dpm (± SEM)
20% (v/v) 1-butanol: 530 ± 91 mean dpm (± SEM)
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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