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Diss Factsheets
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EC number: 240-973-0 | CAS number: 16919-58-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- not stated
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: similarities to OECD test guideline 476 and data are scientifically acceptable
Data source
Reference
- Reference Type:
- publication
- Title:
- Platinum-induced mutations to 8-azaguanine resistance in Chinese hamster ovary cells.
- Author:
- Taylor R. T. et al.
- Year:
- 1 979
- Bibliographic source:
- Mutation Research 67, 65-80
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- GLP compliance:
- not specified
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- Dipotassium hexachloroplatinate
- EC Number:
- 240-979-3
- EC Name:
- Dipotassium hexachloroplatinate
- Cas Number:
- 16921-30-5
- Molecular formula:
- Cl6Pt.2K
- IUPAC Name:
- dipotassium hexachloroplatinate(2-)
- Test material form:
- other: In [presumably aqueous] solution
- Details on test material:
- Potassium hexachloroplatinate (CAS 16921-30-5)
Purchased from Chemical Procurement Labs, Inc.
Stored in the dark; CAS not provided.
Constituent 1
Method
- Target gene:
- HGPRT locus
Species / strain
- Species / strain / cell type:
- Chinese hamster Ovary (CHO)
- Details on mammalian cell type (if applicable):
- CHO-S cells, a proline-requiring Chinese Hamster Ovary cells adapted to suspension culture
- Metabolic activation:
- without
- Test concentrations with justification for top dose:
- Including 10 and 60 uM [possibly 1-100 µM]
- Vehicle / solvent:
- Presumably distilled water
Controls
- Untreated negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- ethylmethanesulphonate
- Details on test system and experimental conditions:
- Briefly, the test substance (or ethyl methanesulphonate) were added to exponentially growing cells, and the cultures were incubated at 37 deg C for 20 hours in the dark. The cells were then harvested by centrifugation and resuspended in mutagen-free medium at a density of 50,000/ml to begin the expression growth in suspension. Growth was monitored, and every 48 hours the cells were centrifuged and an aliqout transferred to fresh medium (50,000/ml) containing 10 µM of the test substance. After 5, 10, 15, 20 and 30 population doublings, the cells were harvested and plated for survival, and subcultured for mutant expression growth in fresh medium to determine the 8-AGR mutant frequency at the HGPRT locus [and possibly the OUAR mutants]; results were compared to control. It seems that the test substance was also tested at 60 µM and, after 7 population doublings, the OUAR and 8-AGR mutant frequencies were evaluated.
- Evaluation criteria:
- Only data for 10 and 60 uM are presented.
Resistant mutant frequencies were presumably compared to control. - Statistics:
- None reported.
Results and discussion
Test results
- Species / strain:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Remarks:
- weak
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- not significant at 10 uM; growth/cloning efficiency reduced by 50% at 34/50 uM, respectively
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- A 2-3-fold increase in the 8-AGR mutant frequency versus the spontneous control was seen upon repeated subculture. This increase was apparent after 10 population doublings.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive without metabolic activation, weak with prolonged exposure
In a well-conducted in vitro mammalian gene mutation test using CHO cells, dipotassium hexachloroplatinate was weakly mutagenic when tested up to cytotoxic concentrations, in the absence of metabolic activation. - Executive summary:
Dipotassium hexachloroplatinate was tested for genotoxicity in an in vitro mammalian gene mutation test using Chinese Hamster Ovary (CHO) cells deficient in HPRT (hypoxanthine-guanine phosphoribosyl transferase). Cells were tested only in the absence of a metabolic activation system.
A 2-3-fold increase in the 8-AGR mutant frequency versus the spontaneous control was seen upon repeated subculture [prolonged exposure] with a non-cytotoxic concentration of 10 μM. This increase was apparent after 10 population doublings; the trend towards it was observed at 5 population doublings.
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Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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