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EC number: 213-485-0 | CAS number: 957-68-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- September 19, 1994 to November 30, 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study (OECD 401) with acceptable restrictions.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 (Acute Toxicity (Oral))
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- acute toxic class method
- Limit test:
- yes
Test material
- Reference substance name:
- 3-acetoxymethylen-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
- EC Number:
- 213-485-0
- EC Name:
- 3-acetoxymethylen-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
- Cas Number:
- 957-68-6
- Molecular formula:
- C10H12N2O5S
- IUPAC Name:
- 3-acetoxymethylen-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
- Test material form:
- solid - liquid: suspension
- Details on test material:
- - Name of test material (as cited in study report): 7-ACA
- Physical state: white powder
- Analytical purity: 95%-102% (HPLC)
- Impurities: 7-ACA 4.7%
- Batch No.: 3701586
- Expiration date of the batch: stable under conditions of storage
- Storage condition of test material: in a refrigerator in the dark
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Animals:
Supplier: Forschungsinstitut für Versuchstierzucht, A-2325 Himberg
Age: approximately 8 weeks at time of administration
Environment:
Room temperature: average of 22 °C,
Humidity: average of 55 %,
Air exchange: 12 per hour,
Light artificial light from 6 a.m. to 6 p.m.,
Cages: Single caging in Makrolon cages type III,
Bedding material: Aspen wood chips, type "4 HV",
Feed: Altromin 1314 ff, gamma irradiated with 10 kGy 60Co, ad libitum,
Water: tap water, offered in Makrolon bottles with stainless steel canules, ad libitum,
Acclimatization: 6 days
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: Carboxymethylcellulose, sodium salt
- Details on oral exposure:
- Peroral administration was performed within maximum 30 minutes after preparation of the test substance suspension once in the morning by stomach intubation using a metal gavage. The test substance suspension was stirred during the time of administration.
- Doses:
- 2000 mg/kg body weight (20 ml/kg)
- No. of animals per sex per dose:
- 5 males and 5 females
- Control animals:
- no
- Details on study design:
- Observations in life: Behaviour, reactions and physical signs of the animals were observed 0-0.5, >0.5-1, >1-2, >2-4 and >4-6 hours after administration of the test substance (p.a.) and then at least once a day for a total of 2 weeks.
Body weight and body weight gain: Body weight was determined before administration, 7 days p.a. and 14 days p.a.
Body weight gain was calculated for each week of the study, i.e. 0-7 d p.a. and 7-14 d p.a.
Necropsy: All animals were sacrified by CO2 14 days p.a. and examined macroscopically in an attempt to detect possible residual lesions.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- All animals (5 males and 5 females) survived until the scheduled termination of 14 days p.a.
- Clinical signs:
- Males: 3/5 animals were normal during the whole obser vation period. In the affected males chromodacryorrhoea, an unspecific sign of general malaise, was noted on day of administration of the test substance for about 2 hours.
Females: All animals, i.e. 5 females, were normal during the whole observation period. - Body weight:
- Males and females: At the scheduled examination terms, body weight and body weight gain were inconspicuous in all animals.
- Gross pathology:
- Males and females: All animals were normal at terminal necropsy.
- Other findings:
- Sex differences: Signs in life and post mortem findings revealed no differences between the sexes in the response to the test substance.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The LD50 is above 2000 mg/kg bw.
- Executive summary:
An acute oral toxicity study with rats was performed. The LD50 is above 2000 mg/kg bw.
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