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EC number: 228-250-8 | CAS number: 6197-30-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Dermal absorption
Administrative data
- Endpoint:
- dermal absorption
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- no data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 428 (Skin Absorption: In Vitro Method)
- Principles of method if other than guideline:
- In vitro penetration studies using human and porcine skin
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Octocrilene
- EC Number:
- 228-250-8
- EC Name:
- Octocrilene
- Cas Number:
- 6197-30-4
- Molecular formula:
- C24H27NO2
- IUPAC Name:
- 2-ethylhexyl 2-cyano-3,3-diphenylacrylate
- Details on test material:
- - Name of test material (as cited in study report): Uvinul N 539
No further data
Constituent 1
- Radiolabelling:
- no
Test animals
- Species:
- other: pig and human
Administration / exposure
- Vehicle:
- other: oil water emulsion
- Duration of exposure:
- 16 hours
- Doses:
- 3 mg/cm² (8% test substance)
- Details on in vitro test system (if applicable):
- Human Skin
SKIN PREPARATION
- Type of skin: Human caucasian skin from mammalian or abdominal surgery of females aged between 20 and 65 years
- Preparative technique: freshly dermatomed
- Thickness of skin: 350 ± 50 µm
- Membrane integrity check: yes: visually (no cuts, scars, reddish marks, spots, injuries), TEWL
- Storage conditions: 4°C for 24 hours
PRINCIPLES OF ASSAY
- Diffusion cell: static
- Receptor fluid: 1% of bovine serum albumin, 0.9 % of NaCl, 0.02 % of KCI, 0.04 % of gentamicin sulfate and 0.8% of phosphate buffer in water; pH 7.4.
- Test temperature: mean skin membrane temperature of 32 °C
- Samples assessed: Tapes (16 strippings); epidermis, dermis, receptor fluid, washing solution
Pig Skin
SKIN PREPARATION
- Type of skin: Unboiled back skin of selected female pigs ( 130 days old, weighing about 100 kg). Fresh skin from 6 different animals was used in triplicate.
- Preparative technique: freshly dermatomed
- Thickness of skin: 700 ± 50 µm
- Membrane integrity check: yes: visually (no cuts, scars, scratches, injuries), TEWL
PRINCIPLES OF ASSAY
- Diffusion cell: static
- Receptor fluid: 1% of bovine serum albumin, 0.9 % of NaCl, 0.02 % of KCI, 0.04 % of gentamicin sulfate and 0.8% of phosphate buffer in water; pH 7.4.
- Test temperature: mean skin membrane temperature of 32 °C
- Samples assessed: Tapes (16 strippings); epidermis, dermis, receptor fluid, washing solution
Results and discussion
- Total recovery:
- Pig skin experiments: 90+/-2% recovery
Percutaneous absorptionopen allclose all
- Time point:
- 16 h
- Dose:
- 3 mg/cm²
- Parameter:
- percentage
- Absorption:
- ca. 0.2 %
- Remarks on result:
- other: Human Skin: Application of test substance as 8% in formulation
- Time point:
- 16 h
- Dose:
- 3 mg/cm²
- Parameter:
- percentage
- Absorption:
- ca. 3 %
- Remarks on result:
- other: Porcine Skin: Application of test substance as 8% in formulation
Any other information on results incl. tables
Pig skin - Distribution of the total amount of Caffeine and Uvinul N 539 in six skin samples
Mean % of applied amount (confidence interval of 95%)
Octocrilene Uvinul N539 | Caffeine | |
Surface | 74 (4) | 83 (2) |
Stratum corneum | 14 (3) | 5.0 (1) |
Epidermis | 2.8 (0.9) | 3.0 (1) |
Dermis | 0.3 (0.1) | 1.0 (0.5) |
Receptor medium | 0.0 (0) | 0.6 (0.4) |
Recovery | 90 (2) | 93 (4) |
Caffeine |
% of applied dose |
|||||
skin 1 |
skin 2 |
skin 3 |
skin 4 |
skin 5 |
skin 6 |
|
loss |
3.28 |
7.56 |
9.39 |
4.35 |
6.88 |
9.37 |
surface |
92.63 |
89.19 |
82.31 |
85.26 |
84.16 |
82.24 |
SC |
1.91 |
1.09 |
4.85 |
5.49 |
3.82 |
4.72 |
epidermis |
0.64 |
1.28 |
2.12 |
2.12 |
3.38 |
1.99 |
dermis |
0.65 |
0.39 |
0.78 |
1.41 |
1.13 |
0.69 |
receptor |
0.89 |
0.49 |
0.45 |
0.36 |
0.64 |
0.98 |
Uvinul N 539 |
% of applied dose |
|||||
skin 1 |
skin 2 |
skin 3 |
skin 4 |
skin 5 |
skin 6 |
|
loss |
8.04 |
10.47 |
7.15 |
10.12 |
10.96 |
10.82 |
surface |
75.02 |
75.53 |
72.9 |
73.37 |
67.38 |
76.98 |
SC |
13.78 |
10.83 |
15.15 |
14.3 |
18.89 |
9.8 |
epidermis |
2.78 |
2.95 |
4.34 |
2 |
2.4 |
1.03 |
dermis |
0.38 |
0.22 |
0.46 |
0.21 |
0.39 |
0.37 |
receptor |
0 |
0 |
0 |
0 |
0 |
0 |
Comparison between the repartition profiles in pig an human skin
skin compartment |
µg of applied substance per cm² skin |
|||
Caffeine |
Octocrilene Uvinul N 539 |
|||
pig n = 12 |
human n = 12 |
pig n = 18 |
human n = 9 |
|
surface |
139 ± 12 |
82 ± 7 |
157 ± 15 |
216 ± 17 |
SC |
8 ± 2 |
2 ± 2 |
30 ± 8 |
13 ± 6 |
epidermis |
4 ± 2 |
0.13 ± 0.08 |
6 ± 2 |
0.3 ± 0.7 |
dermis |
1.7 ± 0.9 |
0.3 ± 0.2 |
0.7 ± 0.2 |
0.3 ± 1.0 |
receptor |
1.0 ± 0.6 |
1.4 ± 0.9 |
0.0 ± 0.0 |
0.0 ± 0.0 |
Considering the concentration of the test substance (8%) and the amount applied (3 mg/cm²), 5.4%, 0.125% and 0.125% of the applied dose was found in human stratum corneum, epidermis and dermis respectively. No octocrilene was detectable in the receptor fluid.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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