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EC number: 244-073-9 | CAS number: 20837-86-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: study was preformed according to GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Lead cyanamidate
- EC Number:
- 244-073-9
- EC Name:
- Lead cyanamidate
- Cas Number:
- 20837-86-9
- Molecular formula:
- CN2.Pb
- IUPAC Name:
- λ²-lead(2+) (azanidylenemethylidene)azanide
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: S. typhimurium LT2 TA 1535, TA 97a, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 liver mix
- Test concentrations with justification for top dose:
- Seven concentrations of the test item, suspended in deionised water (ranging from 5011 to 4.6 µg/plate) were used. Five genetically manipulated strains of Salmonella typhimurium (TA 97a, TA 98, TA 100, TA 102 and TA 1535) were exposed to the test item both in the presence and in the absence of a metabolic activation system (S9) for 48 hours, using the plate incorporation method.
Controls
- Untreated negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 2_Amino anthracene (2-AA) in DMSO, Benz-a-pyrene (BaP) in DMSO, 4-Nitro-1,2 phenylene diamine in DMSO, Sodium azide in DMSO
Results and discussion
Test results
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
The test item didn’t show mutagenic effects in both experiments. The number of revertant colonies was not increased in comparison with the spontaneous revertants (solvent only).
Cytotoxicity of the test item was not detected. The background lawn was visible and the number of revertants was not significantly decreased.
Therefore it can be stated, that under the test conditions, the test item Cyanamide, lead(2+) salt (1:1) is
not mutagenic in the Bacterial Reverse Mutation Test usingSalmonella typhimurium,strainsTA 97a, TA 98, TA 100, TA 102 and TA 1535.Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The presented data are reliable and adequate. - Executive summary:
The test item is considered not mutagenic for the reasons given above.
Also, the test item didn’t show any cytotoxicity towards the bacteria.
The concentration of the element Pb in the highest concentrated test solution (50 g test item/L, nominal) was determined under non-GLP-conditions in the first and the second experiment.
The confirmation tests of the genotype didn’t show any irregularities. The control of the titre was above the demanded value. The numbers of revertant colonies of the positive controls were in the range of the historical data of the laboratory (see page39) and were definitely increased in comparison with the negative controls, as well as showing mutagenous potential of the diagnostic mutagenes.
Spontaneous revertants were within the normal range in comparison with the historical data of the LAUS GmbH.
For these reasons, the result of the test is considered valid.
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